Microbiology of surgical site infection in closed upper limb fractures: Data from a prospective trauma registry.

The current study aims to determine the rate of surgical site infection, causal microorganism, and antibiotic sensitivity pattern in operated upper limb closed fractures at the Aga Khan University Hospital, Karachi. Cases presenting between June 2015 to October 2019, were selected from a single-centre, longitudinal, prospective orthopaedic trauma registry. Infection rate, causal microorganism, and antibiotic sensitivity pattern were determined up to six months after surgery. From among a total of 376 closed fractures, 12 encountered surgical site infection with some having late onset, giving an infection rate of 3% which is 1% higher than the international benchmark. Microorganism culture was performed on 5 (42%) patients out of which 2 (40%) were positive. Frequently used prophylactic antibiotics were first generation Cephalosporin and Co-amoxiclav in 9 (75%) patients, but all other patients required other antibiotic categories. Five patients required implant removal with antibiotic coverage. K-wire insertion required prolonged antibiotic treatment. Most of the cultures were negative in spite of the presence of infection.

Comparative analysis of dental pulp stem cells and stem cells from human exfoliated teeth in terms of growth kinetics, immunophenotype, self-renewal and multi lineage differentiation potential for future perspective of calcified tissue regeneration.

Background and Objectives: Owing to high proliferation rate, multipotency and self-renewal capability, dental pulp stem cells (DPSC) and stem cells from human exfoliated teeth (SHED) have become stem cell source of choice for cell based regenerative therapies. We aimed to compare DPSC and SHED as stem cell sources with a future use in regeneration of calcified tissue. Methods: Explant derived human DPSC (n=9) and SHED (n=1) were cryopreserved, thawed and expanded for analysis of population doubling time, colony forming unit assay and efficiency. A growth curve was plotted to determine population doubling time, while colony forming numbers and efficiency was determined at plating cell densities of 5.6, 11.1 and 22.2 / cm2. The isolated cells were characterized for the presence of stem cell markers by immunophenotyping and immunofluorescence staining, and tri-lineage differentiation. Statistical analysis was performed by Pearson correlation, Exponential regression and two way Anova with Tukey test at p<0.05. Results: DPSC and SHED exhibited spindle shaped fibroblast like morphology. SHED was found superior than DPSC in terms of proliferation and colony forming efficiency. Immunophenotypes showed that DPSC contain 62.6±26.3 %, 90.9±14.8% and 19.8±0.1%, while SHED contain 90.5%, 97.7% and 0.1% positive cells for CD90, CD73 and CD105. DPSC were strongly positive for vimentin, CD29, CD73, while reactivity was moderate to weak against CD44 and CD90. SHED expressed vimentin, CD29, CD105, CD90 and CD44. Both were negative for CD45. Upon induction, both cell types differentiated into bone, fat and cartilage like cells. Conclusion: Cultured DPSC and SHED were proliferative and exhibited self-renewal property. Both DPSC and SHED expressed stem cell markers and were able to differentiate into bone, fat and cartilage like cells. Thus, these could be a suitable stem cell sources for cell based regenerative therapies.

Is Hypothyroidism Associated With Outcomes in Fracture Patients? Data From a Trauma Registry.

BACKGROUND: Previous research reports suggesting the susceptibility of patients with hypothyroidism to fragility fractures. The current study aimed to compare injury factors, patient factors, and outcomes of fractures in patients with and without hypothyroidism. METHODS: Study data were extracted from an ongoing single-center prospective orthopaedic trauma registry at a tertiary care hospital. Patients recruited between June 2015 and March 2020 were selected. Patients were grouped into those with and without hypothyroidism, and data on injury factors, management, clinical, and functional outcomes up to 6 mo were compared. Relation of fracture with TSH levels and age was analyzed, and prescription of bone-strengthening supplements was recorded in the hypothyroid group. RESULTS: Among 1347 patients recruited in the trauma registry, 35 patients had hypothyroidism of which 77% were females compared to 30% of euthyroid subjects (P = 0.0001). The most commonly involved anatomic sites identified were the proximal femur and proximal humerus. Low-energy trauma more likely occurred in hypothyroid (71%) compared to 32% of euthyroid subjects (P < 0.001). Osteoporosis was identified in 90% of hypothyroid subjects who underwent a DEXA scan. The clinical and functional outcomes of patients seem to be similar in both groups, possibly due to adequate control of hypothyroidism or the effect of bone-strengthening supplements given to hypothyroid patients. Serum TSH level and age were not related to low-energy trauma in hypothyroid patients. CONCLUSIONS: The current study identified that patients with hypothyroidism presenting with fractures are more likely females with low-energy trauma, involving the proximal femoral, and humeral fractures. Thyroid status was not associated with post-management outcomes.

Functional outcomes in proximal humerus fractures: A prospective registry-based analysis.

The present study evaluated and compared functional outcomes of proximal humerus fractures of different categories and their management with different procedures. Data of this prospective cohort was obtained from the orthopaedic trauma registry at a tertiary care hospital for the period from June 2015 to October 2019. Sixty-eight adult patients with proximal humerus fracture were identified out of which 57 (84%) had been operated. Functional outcomes were assessed up to 12-month follow-ups and were compared with different treatment groups, fracture category, and between isolated versus proximal humerus with additional upper limb fractures. At 3-month follow-up, there was significantly better outcomes in Proximal Humeral Internal Locking System (PHILOS) treatment group as compared to PHILOS with bone graft/BMP (p=0.041). PHILOS combined with bone graft/BMP was associated with delayed recovery compared to other management methods. There was non-significant difference in functional outcomes between isolated versus proximal humerus fractures associated with other upper limb fractures, among different fracture categories and between genders.

Prevention of falls in hospital: Audit report from a Tertiary care hospital of Pakistan.

OBJECTIVE: The study aims to assess the fall incidents in past 5 years and fall assessment practices at the Aga Khan University Hospital, Karachi. METHODS: We performed a single-center retrospective audit at Aga Khan University Hospital from October 2019 to December 2019. A list of all patients admitted to Aga Khan University Hospital under the Musculoskeletal and Sports Medicine Service Line was obtained using the Hospital Information Management System (HIMS) from Jan 2017 to June 2018. Data including fall assessment scores was collected retrospectively from medical record files. RESULTS: A total of 1499 patients were admitted during this time period, of whom 5 patients had a fall incident during their hospital stay. The mean Morse Scale scores of patients who had a fall was 50 ±16 whereas, patients with no fall incidence had mean score of 31±22. Fall assessment was documented in nursing notes for 100% of the patients. CONCLUSIONS: Our findings show that fall policy is implemented strictly within our hospital. In order to reduce the risk of a fall further, more in-depth assessment of high risk patients with involvement of physicians and physiotherapists earlier on in the process for high risk patients may be beneficial.

Strengthening Orthopaedic care at national level: Output of a structured residency programme at Aga Khan University.

This descriptive review of the output of the orthopaedic residency programme of Aga Khan University, Karachi, comprised information regarding the number of graduated residents and their educational background which was retrieved from departmental records. Information about their work location, subspecialty, current working status, participation in medical camps and national disaster relief efforts were obtained from various sources, including fellow surgeons, and social media profiles. From 1989 to 2017, a total of 48 residents graduated from the programme, with only 2(4.2%) of them being females. Overall, 19(39.6%) residents hailed from areas outside Karachi; 28(58.3%) belonged to Karachi; 1(2%) came from Kenya; 41(85.4%) remained to serve in Pakistan working mostly in tertiary healthcare centres; and 7(14.6%) moved abroad on consultancy and teaching assignments. Subspecialty training had a general trend towards general orthopaedics and trauma 21(43.7%), followed by arthroplasty surgery 13(27%).

Patient outcomes association with patient factors and care provided: trauma registry derived cohort study.

OBJECTIVE: To determine the association of delay in treatment with injury-specific patient outcomes. METHODS: This was a single-center, longitudinal cohort study on orthopaedic trauma registry. Data on patients enrolled between June 2015 and June 2018 were analyzed. Data was collected from admitted consenting patients' medical records. Definitive surgical care provided after 24 hours was considered as 'delayed surgical treatment'. Outcomes of patients were serially assessed on follow-up visits up to 12 months using injury-specific scoring system. RESULTS: A total of 789 patients, were enrolled with 856 upper or lower extremity injuries altogether; in 67 cases both extremities were involved. Surgery was done in 90% while 10% were managed conservatively. A delay in the surgical procedure was experienced by 185(23%) patients. Mortality was 3.28% (6 of 185) in the delayed treatment group and 1% (6 of 603 patients) in the early treatment group (p=0.046). In proximal femur there was a nonsignificant trend towards better outcomes in the early treatment group at 3 and 12 months (p=0.06), while in Tibial shaft fractures, there was a non-significant trend towards better outcomes in the delayed treatment group at 3 and 6-months (p=0.09). There was no association between treatment delay for distal radius and proximal humerus fractures and their outcomes. CONCLUSIONS: Our trauma registry model provides outcomes data enabling identification of patient subsets who did not achieve good outcome, and suggests possible role of delay in surgical treatment beyond 24 hours in the outcomes..

Functional and clinical outcomes of open versus closed radius and ulna shaft fractures in adults: A prospective cohort study.

OBJECTIVE: To compare functional and clinical outcomes of open versus closed radius ulna shaft fractures in adults treated by internal fixation. METHODS: A prospective cohort study was conducted on patients presenting with traumatic radius and ulna shaft fractures to Aga Khan University and undergoing internal fixation between July 2015 to June 2019. Data was extracted from an ongoing orthopaedic trauma registry. Functional and clinical outcomes were assessed by Price et al. criteria at 6 weeks, 3, 6 and 12 months follow-up. Outcome scores of open versus closed fractures were compared. RESULTS: Twenty-nine adult patients with isolated radius and ulna shaft fracture were identified. Cause of injury was road traffic accident in 18 (62%) and fall in 11 (38%) patients. Seventeen (59%) were closed and 12 (41%) were open fractures. At 6week follow-up, better outcomes were observed in closed fracture group (p=0.01) with near-full range of motion and activity in 10(83%) patients as compared to 3(27%) in the open fracture group. No significant difference in outcomes was observed at 3 months and thereafter. CONCLUSIONS: Earlier recovery of function at 6 weeks was observed in majority of patients in the closed fracture group. Our data shows that good-excellent functional and clinical results are achievable by internal fixation in both open as well as closed fractures of the shaft of radius and ulna in adults.

Comparison of intraarticular distal humerus fracture outcomes treated with or without olecranon osteotomy - A case series.

A case series was extracted from the trauma registry at Aga Khan University Hospital from the period June 2015 to June 2019. Included were 16 adult patients who presented with intra-articular distal humerus fracture type C2. The functional, clinical and radiological outcomes of fractures treated with or without olecranon osteotomy up to 12 months follow-up were compared. Outcomes were assessed at 6 weeks, 3, 6 and 12 months re-visits. Among the 16 studied patients, 9 (56%) were males and 7 (44%) were females. In the group without osteotomy, there was a good functional and clinical outcome with a mean Quick Disability of the Arm, Shoulder and Hand score of 32±30 at 3 months post-procedure. Bone healing was noticed at 6 months after surgery. In the osteotomy group, 50%-70% bone union was seen at 3 months post-surgery while fair functional and clinical outcome was achieved at 6 months after surgery.

Iatrogenic trauma following percutaneous and minimally invasive surgical interventions.

Technological progress has changed the landscape of surgical practice. Minimally invasive surgery (MIS) and percutaneous interventions (PC) are constantly replacing open procedures. This reduces hospital stay and allows quicker recovery. The application of MIS should follow the good medical practice dictum by Hippocrates i.e. "First do no harm". To remain abreast with new procedures, the medical personnel are required to update and enhance their knowledge and skill. To ensure safety, the innovations are rigorously tested and tried. The learning curve of MIS is shortened by simulator training and proctorship. Credentialing processes are in place to enhance safe delivery of care. Despite of all these measures MIS and PCI are associated with adverse effects. The purpose of this article is to overview the iatrogenic trauma associated with MIS and PCI in major surgical subspecialties.

Can alternate-day Statin regimen minimize its adverse effects on muscle and tendon? A systematic review.

OBJECTIVE: To review evidence-based data with respect to safety and efficacy of alternate-day statin therapy in dyslipidaemia compared to the standard daily dose. METHODS: The literature review was conducted at Aga Khan University Hospital, Karachi from July, 2016 to August, 2017. Electronic database search was carried out to compile available literature using PubMed, Excerpta Medica database and Google Scholar. The most relevant evidence-based research articles published over 10 years were selected. The latest search was dated August 03, 2017. RESULTS: A total of 2,074 articles were initially located. Alternate day statin regimen was reported in 53% of articles. Adverse effects on muscle and tendon were reported in 69% of articles. After scrutiny, 19(0.9%) studies covering alternate-day statin-mediated muscle and tendon disorders and 9(0.4%) studies encompassing the potential pathophysiological mechanisms of statin-associated muscle and tendon injury were selected. Except pravastatin and lovastatin, alternate-day statin therapy was almost as effective in lowering total cholesterol, low-density lipoprotein cholesterol and triglycerides as the daily dosing with low incidence of muscle toxicity and tends in opathy. CONCLUSIONS: Alternate-day statin regimen was found to be very well tolerated and might be an effective and safe remedy in clinical practice.

Analysing outcomes through orthopaedic trauma registry - A prospective cohort study.

OBJECTIVE: To develop a registry for recording injury-specific data to identify gaps and improve care. Methods: The prospective cohort study was conducted at Aga Khan University Hospital, Karachi, from June 2015 to July 2018 though enrollment of patients with limb trauma is continuing to date. Data on injuries and management related to Tibia shaft fractures was collected from medical records, and outcomes were assessed on follow-up visits. Internationally validated injury-specific scores were utilised for assessing functional, clinical and radiological outcomes. SPSS version 19 was used for data analysis. Results: There were 763 patients with 825 limb injuries. Of the injuries, 310(37.6%) related to upper limbs and 515(62.4%) to the lower limbs. Management was surgical for 741(89.9%) and conservative for 84(10.1%) injuries. Overall, 12(1.57%) patients died, and in 7(0.91%) cases mortality was unrelated to trauma and its management. There were 105 patients with tibia shaft fractures. Of them, 88(83.8%) were males and 17(16.2%) were females. At one-year follow-up excellent-to-good results were 12(92%) for intramedullary nailing followed by 7(78%) for open reduction and internal fixation. Conclusion: Registry data can be used to develop preventive strategies and to improve management protocols.

Workplace violence against doctors involved in clinical care at a tertiary care hospital in Pakistan.

A study was conducted to determine perceptions, attitudes and experience of workplace violence among residents and faculty at a tertiary care centre in Karachi, Pakistan. An anonymous, electronic, self-administered questionnaire was circulated among all residents and faculty members working at Aga Khan University Hospital, Karachi. A standard questionnaire was devised and used, and workplace violence and its types were defined as per World Health Organization (WHO) definitions. An overall response rate of 44.9% was achieved. A total of 53.4% of the respondents reported being victims of some form of workplace violence with verbal abuse being the most prevalent (41.6%) followed by bullying and threat. Most frequent perpetrators were found to be faculty members followed by patients or their attendants. Specialty of respondents was found to be significantly associated with verbal abuse and significantly more females were subjected to sexual harassment, while ethnicity was found to be significantly associated with racial harassment. The results correspond to previously available literature, while they also highlight some findings unique to our culture. We suggest that measures should be taken as per WHO and Joint Commission International Accreditation ( J CIA) recommendations to prevent workplace violence across the country.

Isolation and culture of dental pulp stem cells from permanent and deciduous teeth.

OBJECTIVE: To isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent and deciduous teeth. METHODS: It was an in-vitro experimental study. Human teeth were collected from 13 apparently healthy subjects including nine adults and four children. After decoronation dental pulps were extirpated from teeth and cultured via explant method in a stem cell defined media. Data was analyzed by descriptive statistics. RESULTS: As above MSCs emerged exhibiting fibroblast-like morphology. In vitro culture was positive for 100% (9/9) and 75% (3/4) of the permanent and deciduous teeth respectively. First cell appeared from deciduous teeth pulp in 10±6.2 days while permanent teeth pulp took 12.4±3.7 days. Together, 26.6±3.6 and 24.5±3.5 days were required for permanent and deciduous tooth pulp stem cells to be ready for further assays. CONCLUSIONS: The protocol we developed is easy and consistent and can be used to generate reliable source of MScs for engineering of calcified and non-calcified tissue for regenerative medicine approaches.

Therapeutic uses of pineapple-extracted bromelain in surgical care - A review.

Bromelain is an extract obtained from the pineapple plant and is used as a traditional folk remedy for several ailments. In this review, a comprehensive electronic database search was carried out to compile available literature on therapeutic implications of bromelain. Pharmaceutical value of bromelain has been demonstrated in different surgical sub-specialties. Diverse biological processes like anti-inflammatory, anti-oedematous, analgesic, anti-thrombotic, exfoliation etc. are involved in bromelain's therapeutic actions, mediated through the kallikrein-kinin and arachidonic acid pathways as well as through effects on cell mediated immunity. Bromelain equals non-steroidal anti-inflammatory drugs as an anti-inflammatory agent, but has been shown to have fewer side effects. In Europe it is approved for oral and topical use, mainly for surgical wounds, inflammation due to trauma and surgery, and debridement of deep burns. Literature suggests a promising role of bromelain in surgical care. More clinical trials to establish its utility as an anti-inflammatory agent in surgical care are recommended.

Lower red cell transfusion rates with use of tranexamic acid in single-stage bilateral total knee arthroplasty: A retrospective audit.

Bilateral total knee arthroplasty (BTKA) patients may require blood transfusion which has its risks. Anti-fibrinolytic drugs such as aprotinin, aminocaproic acid and tranexamic acid (TXA) have reduced transfusion requirements in major surgery. This retrospective audit was performed to assess effectiveness of TXA in reducing blood transfusion rate in single-stage sequential BTKA cases operated by a single surgeon. Records of 91 patients given TXA and 80 controls who were operated before 2012 and not given TXA were reviewed. TXA was given 15mg/kg intravenously (IV) before tourniquet deflation and 3 hours postoperatively.Blood transfusion was done in 9(10%) patients in the TXA group compared to 20(25%)in the control group (p<0.01). One (1.25%) patient in the control group had non-fatal pulmonary embolism.TXA appeared to be effective in decreasing post-operative blood loss and requirement for blood transfusion after single-stage BTKA.

Intertrochanteric hip fractures in octogenarian patients: Do we need to rethink fixation strategy?

OBJECTIVE: To compare outcomes in octogenarians with younger patients up to 1 year after undergoing dynamic hip screw fixation for intertrochanteric fractures to see the effectiveness of the implant. METHODS: The retrospective case-control study was conducted at The Aga Khan University and comprised records of patients who underwent dynamic hip screw surgery between January 1, 2010, and December 31, 2012. They were divided into two groups based on their age:. Group A <80 years; and Group B > 80 years. All patients had a one-year follow-up> Data including mortality, morbidity, radiological healing time, postoperative ambulatory status and Harris hip score were recorded. RESULTS: Of the 150 patients, 114(76%) were in Group A and 36(24%) were in Group B. Up to 25(70%) of octagenarians dropped their ambulatory ability by one or two levels, whereas 107(94%) of Group A patients were able to either maintain their pre-injury ambulatory ability or dropped their ambulation by one level only (p=0.02). Postoperative complications were higher in Group B 9(25%) compared to Group A 4(4%). One-year mortality was also significantly higher in Group B 8(22%) compared to Group A 9(8%) (p=0.03). CONCLUSIONS: Octagenarians undergoing internal fixation with dynamic hip screw had higher frequency of complications and death compared to younger patients.

Trends in management of neck of femur fracture.

OBJECTIVE: To identify trends related to surgical procedure for the management of neck of femur fracture to see how the choice is changing over time in relation to different age groups. METHODS: The retrospective audit was conducted at Aga Khan University Hospital, Karachi, and comprised data of all patients who presented with neck of femur fracture from 1995 to 2014. Patients with open fractures and those having external fixators were excluded. Data was retrieved from the hospital database, and analysed using SPSS 17. RESULTS: Records of 1039 patients were reviewed. The proportion of patients in the 70+ years age group significantly increased from 45% to 52% over the two decades (p=0.033). Proportion of patients undergoing total hip replacement increased by more than 3 times from 2.6% to 8% (p<0.001).Internal fixation was the most common procedure in patients aged <50 (p<0.001), and hemiarthroplasty for those aged > 50 years (p<0.001). Total hip replacement was seen in its highest proportion (15%) in patients aged 40-49 years (p=0.006). Hemiarthroplasty showed a dramatic decrease in the age group 50-59 years (from 57% to 40%). Total hip replacement showed a steady increasing trend over the last 20 years, most prominently in people in their 50s and 60s (from 1% to 18%; p<0.001). CONCLUSIONS: There is apparently a rising trend in number of patients presenting with neck of femur fracture. Total hip replacement has a rising trend and partial hip arthroplasties have a decreasing trend when it comes to treatment of neck of femur fractures.

Calcaneal fractures: An audit of radiological outcome.

OBJECTIVE: To assess radiological outcome of management of calcaneal fractures. METHODS: The retrospective study was conducted at Aga Khan University Hospital, Karachi, and comprised data of calcaneal fractures managed between February , 2008 and February, 2014 Cases were identified through medical records, and X-rays were reviewed through digital radiology archive. Bohler\'s angle, Gissane\'s angle, calcaneal height and width were assessed digitally on pre-operative and post-operative X-ray images. RESULTS: Of the27 patients, only 1(3.7%) was female. The cause of fracture was fall from height 15(56%), road traffic accidents 7(26%) and bomb blasts5(19%). Tongue type fractures were 16(59%) and 11(41%) were joint depression type. There was a significant mean increase in Bohler\'s angle (p<0.001) and mean decrease in calcaneal width (p=0.023). Gissane\'s angle and calcaneal height increased marginally (p> 0.05 each). CONCLUSIONS: Measurable improvement in anatomical parameters is possible with surgery, which is a prerequisite for good functional results.

Variability of magnification on digital pelvic radiographs from patients with fractures of the femoral neck - a retrospective audit.

OBJECTIVE: To determine the variability in magnification of radiographs in an emergency setting. METHODS: The retrospective study was conducted at Aga Khan University Hospital, Karachi, and comprised records of patients who underwent Austin Moore hemiarthroplasty over a two-year periodfrom 1st January 2006 to 31st December 2007. Magnification factor was determined using measurements obtained from preoperative and postoperative radiographs and comparing them with the actual size of implant used. Intra-observer and inter-observer reliability of measurements were calculated. SPSS 19 was used for data analysis. RESULTS: Of the 63 patients studied, 25(39.7%) were males and 38(60.3%) were females with an overall mean age of 69.8±12 years. The mean implant size used was 46mm±4mm. Preoperative magnification was 8%±4%, resulting in an overestimation of implant size by 4.2±2.3mm. Postoperative magnification was 13%±4%, resulting in an overestimation of 5.9±1.9mm.Prediction using fixed scaling of 15% resulted in a correct estimation of implant size for only 15(24%) patients. CONCLUSIONS: Digital radiographs of the pelvis exhibited variable amount of magnification along with an inconsistency in magnification on repeat examination.