Anti-oxidant and antibacterial activities of novel N-sulfonylphtalimide in an ovalbumin-induced inflammation in Wistar rats.

Phthalimide and N-substituted phthalimide have a special structure that helps them to be pharmaceutically useful and biologically active. In this study, we investigated the antioxidant, anti-inflammatory and antibacterial effects of a synthetic phthalimide-containing derivative in an experimental asthma model. In vitro determination of antioxidant and chelating activity was carried out by spectrophotometric methods. The in vivo antioxidant activity was carried out in Wistar rats sensitized to ovalbumin in the experimental model of asthma. Our results reveal that that the synthesized N-sulfonylphthalimide molecule has a scavenging capacity against the free radical 2,2-diphenyl-1-picryl-hydrazyl (DPPH•) and a chelating activity on ferrous ions and revealed its protective capacity against altered markers of oxidative stress in the experimental asthma model. All the previous results were confirmed by the result of the histopathological study of the liver. Moreover, neo-synthesized N-sulfonylphthalimide 2 showed antibacterial activity against Gram-positive and Gram-negative bacteria with interesting MIC values. Finally, our study highlights the anti-inflammatory, anti-asthmatic and antibacterial effects of the N-sulfonylphthalimide molecule, which could potentially be a drug of choice in asthmatic pathology, especially during bacterial superinfections in the respiratory tract.

In-vivo anti-inflammatory effects of Roman Chamomile (Chamaemelum nobile) aqueous extracts collected from the National Park of El-Kala (North-East, Algeria).

The aim of our study is to evaluate anti-inflammatory effect of Chamaemelum nobile. Aqueous extracts were administrated to Wistar rats in bronchial-inflammation experimentally induced by an allergen and ovalbumin, administered intraperitoneally / intranasally (20mg/kg/day). Experimentation showed disturbances in bronchoalveolar fluid with increased leukocyte and lymphocyte levels as well as IL-4 concentration in the lungs and erythrocytes associated with high lipid peroxidation. There were disturbances in enzymatic and non-enzymatic antioxidant defense system. Lungs histopathological showed an inflammatory lymphoplasmacytic infiltrate, moderate edema of alveoli, vascular congestion and suffusion hemorrhage. Administration of aqueous extract to OVA-sensitized rats caused a significant and very highly significant improvement of MDA levels in lungs, erythrocytes, GSH, GPx, GST, catalase and SOD. We notice a decrease in IL-4 in LBA and lungs alongside reduced inflammatory cell infiltration, mild bronchiolar dilation, mild alveolar edema and normal cell morphology allowing us to conclude on the effectiveness of anti-inflammatory activity of Roman chamomile.