RESUMEN
In this study, the change in the content of the macro and micro elements in the growing wild grazing plant of Calligonum comosum was tracked at the Research and Training Station of King Faisal University in Al-Hassa Governorate, Kingdom of Saudi Arabia. Mineral elements were estimated in aerial parts (plant as a whole, leaves and stem) from January-April 2020. The results showed that the concentration of nitrogen, phosphorus and potassium in the plant as a whole plant > leaves > roots, while the concentrations of calcium, magnesium, manganese, zinc and copper elements in the leaves was higher than other parts whereas the concentrations of these elements of whole plant were higher than the concentrations in roots. The results showed that the plant contents of nitrogen, potassium and zinc were the highest in March, while the concentrations of phosphorus, calcium, iron and copper were in February. The concentrations of magnesium, manganese and copper was the highest in January and April respectively. The values ââof nitrogen, phosphorous, potassium, calcium, magnesium, iron, manganese, zinc and copper ranged from 11.1 to 18.4 g kg-1, 4.17-2.33 g kg-1, 13.73-18.97 g kg-1, 24.50-28.90 g kg-1, 10.40-12.30 gkg-1, 1500-1677 mg kg-1, 45.45-49.29 mg kg-1, 70.70-177.23 mg kg-1, 16.78-73.46 mg kg-1, respectively. Furthermore, the results exhibited that the lowest values of the elements appeared in the plant roots in April. As well as, the distribution of the elements followed the normal life curve from January to April. Besides that, the evaluated elements satisfy the needs of the grazing animals' life in which this type of plant grows.
RESUMEN
There is an extensive search for natural products that can be introduced to broiler rations to improve performance, especially during the unfavorable breeding conditions. Under heat-stress conditions, the immune response seriously deteriorates, which consequently impairs broiler production performance. Thus, the present study aimed to investigate the potentials of Citrullus colocynthis seeds (CCs) supplementation to modulate the immune response of broilers subjected to chronic heat stress. A total of 300 Cobb-500 male broiler chickens aged 21 days were randomly divided into two equal groups and reared under either thermo-neutral condition (24 ± 1 °C) or subjected to cyclic heat stress (34 ± 1 °C for 8 h). Each group was further divided into two groups (5 replicate × 15 chicks) and was fed either the basal diet or the basal diet with 0.1% CCs supplementation. The results showed that heat stress impaired the production performance by lowering the final body weight and feed intake as well as impairing feed conversion. The levels of stress markers (i.e., malondialdehyde, tumor necrosis factor-α and corticosterone) increased (p < 0.05), whereas the activity of antioxidant enzymes decreased in broilers exposed to heat stress. Further, heat stress caused direct suppression of broiler humoral and cell-mediated immune responses. The stimulating index of T and B lymphocytes proliferation, as well as the antibody titer against sheep red blood cells, were significantly (p < 0.05) reduced by heat-stress exposure. However, CCs supplementation to broilers subjected to heat stress improved (p < 0.05) the final body weight, feed intake, and feed conversion ratio (FCR), compared to the non-supplemented stressed group. The cellular and cell-mediated immune response indicators significantly enhanced (p < 0.05) with CCs supplementation. Supplementation of CCs to broilers reared under similar environmental conditions elevated the total white blood cells (TWBCs) count and the broiler stimulating index of T and B lymphocytes. It can be concluded that CC seeds can be effectively used to stimulate the immune response and improve the production performance of broilers reared under heat-stress condition.