Understanding Solid-Based Platelet-Rich Fibrin Matrices in Oral and Maxillofacial Surgery: An Integrative Review of the Critical Protocol Factors and Their Influence on the Final Product.

Platelet-rich fibrin (PRF) is a second-generation platelet concentrate whose use in clinical practice has been widely disseminated. This has led to the development of several commercial protocols, creating great confusion as to the terminology and implications of each of them. This integrative review aims to identify the critical factors of each of the phases of the solid-based PRF matrix protocol and their possible influence on their macro- and microscopic characteristics. An electronic search of the MEDLINE database (via PubMed), Web of Science, Scopus, LILACS, and OpenGrey was carried out. The search was temporarily restricted from 2001 to 2022. After searching, 43 studies were included that met the established criteria. There were numerous factors to consider in the PRF protocol, such as the material of the blood collection tubes, the duration of phlebotomy, the parameters related to blood centrifugation, the time from centrifugation to dehydration of the fibrin clots and their dehydration into membranes, as well as the time to clinical use. These factors influenced the macro- and microscopic characteristics of the PRF and its physical properties, so knowledge of these factors allows for the production of optimised PRF by combining the protocols and materials.

Nuevas tendencias en regeneración tisular: fibrina rica en plaquetas y leucocitos / New tendencies in tissue regeneration: leucocyte-rich platelet-rich fibrin

La regeneración periodontal es la reproducción o reconstitución de una parte perdida o dañada del periodonto con el fin de restaurar su arquitectura y función. En los últimos años se ha puesto de manifiesto el papel clave que juegan las plaquetas en la regeneración tisular, acelerando la cicatrización tanto de tejidos blandos como duros, mediada por la liberación de citocinas y factores de crecimiento durante un tiempo prolongado. La fibrina rica en plaquetas y leucocitos utilizada por primera vez por Choukroun en el 2001 es un concentrado de plaquetas de segunda generación que se obtiene a partir de la propia sangre del paciente, sin el empleo de aditivos, con el fin de conseguir una malla de fibrina que sirva de andamiaje para las sustancias implicadas en la regeneración. El objetivo de este trabajo es el de realizar una revisión y puesta al día en el uso de esta técnica (AU)

Periodontal regeneration is the reproduction or re-enactment of an injured, or lost, part of the periodontium, with the aim of repairing its architecture and main function. The key role of platelets in tissue regeneration has been demonstrated in the last few years. They accelerate healing in both the soft and hard tissues due to the liberation of cytokines and growth factors over a long period. Leucocyte-rich platelet-rich fibrin, used for the first time by Choukroun in 2001, is a second generation platelets extract that is obtained from the patient's own blood, without the need of additives. Its purpose is to attain an autologous fibrin mesh to be used for as a framework for the substances involved in bone regeneration. The purpose of this work is to present a review and an update on the use of this technique (AU)