A multi-residue method for the determination of pesticides in high water content matrices by gas chromatography-single quadrupole mass spectrometry with electron ionisation (EI-GC/MS).

An EI-GC/MS method for the determination of pesticide residues in vegetable matrices with high water content was validated using papaya samples. The validation of a multi-residue pesticide method was in agreement with national and international regulations enabling the Ministry of the Agriculture, Livestock and Food Supply of Brazil to cover a large number of matrices and pesticide residues in its monitoring and control programmes. The extraction used 60 mL of ethyl acetate and 30 g of sample previously processed. After extraction, clean-up of all the extracts was carried out by percolation through GBC cartridges. The samples were then injected in an EI-GC/MS system. Calibration curves were prepared in quadruplet by fortifying blank extracts with a standard solution containing all the pesticides studied at 0.000, 0.005, 0.010, 0.020, 0.030, 0.050, 0.080 and 0.100 mg kg(-1). For the recovery study, blank samples were fortified at 0.010, 0.020, 0.030, 0.050 and 0.080 mg kg(-1) and then submitted to the extraction procedure. The complete procedure was repeated over four different days by two analysts. The regression parameters of calibration curves were calculated for each validation day. Linearity, selectivity, specificity, robustness, limits of detection and quantification were also assessed. The uncertainty was estimated for each analyte at each spike level studied. The method had recoveries between 91% and 105% and precision results ≤ 20%. Limits of quantification were below or equal to the maximum residue limits (MRLs) regulated by Brazilian legislation. The MRLs of the selected pesticides are not regulated by CODEX Alimentarius. The results are also in agreement with SANCO/10684/2009.

Idiopathic megacolon in a teenager treated by laparoscopic rectosigmoidectomy / Megacolon idiopático em adolescente tratado por retossigmoidectomia videolaparoscópica

Abstract Chronic constipation in children and adolescents is relatively common and a reason for consultation with pediatricians and proctologists. Most cases respond to medical treatment. Advanced cases of megacolon and megarectum can be treated surgically by Duhamel technique. This case report describes a 15-year-old patient with chronic intestinal constipation refractory to clinical treatment associated with megacolon and megarectum, which was surgically treated.

Resumo A constipação intestinal crônica em crianças e adolescentes é relativamente comum e motivo de consultas a pediatras e coloproctologistas. A maioria dos casos responde ao tratamento clínico. Casos avançados de megacolon e megarreto podem ser tratados cirurgicamente através da cirurgia de Duhamel. Este relato de caso descreve um paciente de 15 anos de idade com quadro de constipação intestinal crônica refratária ao tratamento clínico associada a megacolon e megarreto, o qual foi tratado cirurgicamente.

Associação entre megacólon chagásico e adenocarcinoma gástrico em uma mulher idosa / Association between chagasic megacolon and gastric adenocarcinoma in an elderly woman

O objetivo deste relato foi apresentar o caso de uma paciente com megacólon chagásico perfurado associado a colite isquêmica, que evoluiu para peritonite fecal aguda. A paciente era uma mulherbranca, 82 anos, com cianose, anasarca, melena, dor, distensão abdominal e sorologia positiva para doença de Chagas. Seu estado evoluiu para o óbito e a autópsia revelou megacólon chagásico com perfuração e peritonite aguda fecaloide purulenta, sendo esta última a causa imediata do óbito. Esteé, provavelmente, um dos poucos relatos de colite isquêmica, megacólon chagásico perfurado e peritonite fecal aguda associada a adenocarcinoma gástrico não diagnosticado antes do óbito.