A thermally robust method of sample sealing for capillary DLS.

Capillary Dynamic Light Scattering (DLS), has recently been introduced as a simple and enabling technique that increases the measurement range of traditional DLS analysis with minimized sample volumes (Ruseva et al., 2018). The previously published protocol for the preparation of samples for analysis within a capillary called for sealing of the capillary end using a clay compound (Ruseva et al., 2019). This material is not, however, compatible with organic solvents, nor with elevated sample temperatures. To extend the uses of capillary DLS to more complex assays like thermal aggregation studies, a new sealing method is demonstrated using a UV curing compound. This further motivates the use of capillary DLS to minimize volumes of destroyed precious samples in pharmaceutical development assays to study thermal kinetics.•Use of UV curing compound to seal capillaries used in DLS to preserve low volumes of sample.

Routine, ensemble characterisation of electrophoretic mobility in high and saturated ionic dispersions.

With the industrialisation of nanoparticle manufacture, the pervasive incursion of nanoparticles into the environment, the need to characterise nano-scale pharmaceuticals and living systems in replicated in vivo conditions, the continuing development of new theories to describe the electro-kinetic behaviour of nano-particles in representative ionic strengths and numerous other applications, there is an urgent requirement to provide simple and effective experimental tools to validate these models and explore new systems. Micro-electrophoresis implemented with a diffusion barrier, which isolates the dispersed phase from the electrode surface, is demonstrated as enabling such measurements for the first time, preventing the catastrophic outgassing, precipitation and sample degradation observed when the dispersed phase is in close proximity to the electrode surface. Using a measurement of a few minute's duration in a standard laboratory light scattering instrument we reproduce the theoretically predicted phenomena of asymptotic, non-zero electrophoretic mobility with increasing ionic strength, the cationic Hofmeister series dependency, charge inversion and a continuously decreasing variation in mobility with pH as molarity increases. Standard operating procedures are developed and included to encourage further work.