Dynamic patterns of expressed genes in granulosa cells during follicular and luteal stages in Egyptian buffaloes.

A better understanding of the molecular mechanisms in granulosa cells (GC) is warranted, during different follicular and luteal developmental stages in buffalo cows. We aimed to (I) study the expression of selected genes in GC during follicular and luteal phases, (II) evaluate correlations between GC gene expression and steroid concentrations {17-beta estradiol (E2) and progesterone (P4)} in follicular fluid (FF), and (III) study effect of ovarian status on follicular population as well as follicular size frequency. Ovaries were collected in pairs from buffaloes (n = 178). Ovaries bearing corpus luteum (CL) were subdivided into hemorrhagic, developing, mature, and albicans. Follicles from luteal groups were classified only into small (< 4 mm) and large (9-20 mm), while follicles from follicular groups were classified into three subgroups: small (< 4 mm), medium (5-8 mm), and large (9-20 mm). The FF and GC were collected for steroid concentrations measurement and gene expression, respectively. In the follicular phase, luteinizing hormone/choriogonadotropin receptor (LHCGR) and cytochrome P450 aromatase (CYP19) in small follicles decreased compared to medium ones. Large follicle showed an increase in LHCGR and CYP19 compared to medium ones. Follicle-stimulating hormone receptor (FSHR) decreased in large compared to medium size follicles. Proliferating cell nuclear antigen (PCNA) increased in small and large follicles. Meanwhile, anti-Mullerian hormone (AMH) and phospholipase A2 group III (PLA2G3) decreased in small and large follicles. The different stages of luteal phase had a profound impact on GC gene expression. There were strong (positive and/or negative) correlations between gene expression and steroid hormones. The different scenarios between expressed genes in GC and steroid concentrations are required for the proper growth and development of follicles and CL.

Reduction of fresh frozen plasma use with a simple education program.

Fifty-six charts of patients who received 307 units of fresh frozen plasma (FFP) were audited to determine the transfusion practice of this component at our 550-bed regional referral hospital. Forty-six patients (82%) were on the surgical service. Indications for FFP transfusion were poorly documented. Forty-nine patients (87%) lacked evidence of a coagulopathy. An educational program addressing appropriate use of FFP was initiated. Over the course of two years, FFP usage dropped by over 1,000 units annually (46%).

Bilateral recurrent nerve paralysis associated with multinodular substernal goiter: a case report.

Substernal goiter is an infrequent occurrence and is found in two to five per cent of all patients undergoing thyroid surgery. These lesions are well known to cause respiratory symptoms and alterations in phonation due to direct compression of airway structures. Infrequently, unilateral recurrent nerve palsy has been reported in patients with substernal goiter. We report a case of bilateral recurrent nerve palsy associated with multinodular substernal goiter in an 89-year-old female who presented in respiratory distress.

Thru-knee amputation: the operation of choice for non-ambulatory patients.

Between 1983 and 1990, 32 consecutive patients had 35 thru-knee amputations for ischemia causing infection or gangrene of the lower leg. Twenty-seven patients were nonambulatory, five had limited mobility, and none were considered to be candidates for rehabilitation with a prosthesis after surgery. The average age was 73 years. Thirty-day hospital mortality was 3 per cent. The incidence of complications was low, and healing was achieved in 97 per cent of survivors. One patients required revision to an above-knee amputation. Average postoperative hospital stay was 8.7 days. It is concluded from this study that thru-knee amputation is the treatment of choice in patients with nonsalvageable ischemic legs and in whom ambulation is not planned after operation.

Metabolic costs of stoopwalking and crawling.

This paper is a study of the metabolic costs of crawling and stoopwalking as performed by trained male and female subjects. After training, male and female subjects crawled and stoopwalked at a range of task speeds and in task postures set at 100, 90, 80, 70, and 60% of each subject's erect stature. It was found that as the task posture became more stooped, or the task speed increased, there were marked increases in metabolic cost. Further analysis found these increases to be due to the task speed within a posture rather than from the task posture. It was also found that in some task postures, the combination of speed and posture resulted in metabolic costs of performance which would be limiting in terms of non-fatigued task performance time.

Ergonomic investigation of letter-carrier satchels: part II. Biomechanical laboratory study.

A biomechanical investigation of four satchels designed for the purposes of manually carrying and delivering mail was conducted. Twenty United States Postal Service carriers participated in the study. The satchels differed primarily in the presence/absence of a waist belt, the number and design of the shoulder straps, and the number of pouches. The biomechanical analyses were comprised of postural (shoulder and hip) deviation measurements, estimated compressive forces at the L5/S1 joint, soft tissue pressure on the shoulder, spinal torsion during mail retrieval, force distribution between the feet, and an anthropometric evaluation. The results indicated that a satchel with two shoulder straps and two pouches was more desirable than the single-pouch satchels from a biomechanics standpoint.

Ergonomic investigation of letter-carrier satchels: part I. Field study.

A field study was conducted to compare four satchels designed for the purposes of manually carrying and delivering mail. The satchels differed primarily in the presence/absence of a waist belt, the number and design of the shoulder strap(s) and the number of pouches. The evaluation consisted of questionnaires and interviews of slightly more than 300 letter carriers employed by the United States Postal Service. The questionnaires focused on 10 main areas of interest; ease of adjustability and handling, comfort, suitability and durability of materials, anthropometric applications, impact on other activities, ease of motion in retrieving mail, ease of motion in carrying the satchel, versatility of use in diverse climates, protection of mail from damage due to rain and snow, and defense against dog attacks. The primary conclusion was that the satchel with two shoulder straps and two pouches, which results in more symmetric loading than the other satchels, is the most desirable of the satchels tested. However, there are problems with the satchel that need to be addressed, such as concerns about the use of the satchel for defense from dog attacks.

Therapeutic efficacy of povidone-iodine (Betadine) and dichloroxylenol (Septocid) in Holstein cows affected with endometritis and/or cervicitis.

The efficacy of either povidone-iodine (Betadine) or dichloroxylenol (Septocid) intrauterine infusions on the treatment of endometritis and/or cervicitis in cows was examined. One hundred and twelve repeat-breeder Holstein cows (aged 3-7 years) were selected for this study. Rectal and vaginal examinations were applied to detect the signs of endometritis and/or cervicitis. Cows were assigned into two groups: the first group (n = 60) was treated with Betadine solution (0.5%) while the second group (n = 52) was given Septocid (0.1%) administered as intrauterine infusion (100-150 ml). Both treatment were repeated at least two times at 7-day intervals and the cows were rechecked. The responding animals were inseminated 10-12 h after oestrus detection using frozen semen from bulls of proven fertility. The success or failure of treatment was evaluated on the basis of the post-treatment conception rate. The relationship of the body weight of cows, the length of the service period (open days) and the number of previous services with the results of treatment with Betadine or Septocid is discussed. The recovery and conception rates obtained after Betadine treatment were better than those obtained after Septocid. Moreover, healthy cows (> or = 500 kg body weight) and those inseminated before post-partum day 180, having no more than 4-7 previous services, responded well to either Betadine or Septocid treatment.

Lifting capacity of workers.

In order to establish a valid criteria on which to establish lifting guidelines, lifting capacity determination must be made. This has been attempted using several approaches mainly psychophysical, physiological, and biomechanical methodologies. Although the data obtained from these approaches yield information about lifting capacity, some conflict in these data can be found. The purpose of this paper is to discuss the three different approaches, the data obtained from these approaches, and some data on the lifting capacity for males and females in the industrial workforce. In addition, the conflicts in these data are also presented.

Modeling in manual materials handling.

Manual materials handling (MMH) account for approximately $15.0 billion of injuries cost in the United States alone. Because of the need to establish manual handling guidelines, different approaches to achieve this goal were pursued. Such approaches are based on biomechanics, work physiology, and psychophysics. In order to better understand the MMH system's behavior, models were developed. These models can be divided into biomechanical models, physiological models, psychophysically based capacity models, safe load handling models, and simulation models. This paper will present a sample of these models and discusses their advantages and disadvantages.

Effect of task variables and their interactions in lifting and lowering loads.

An experiment was designed to study the effect of several task variables on the metabolic energy expenditure and heart rate of individuals performing lifting and lowering tasks. The task variables studied were: load of lift (lower), frequency of lift (lower), height of lift (lower), presence of absence of handles, container height, container width (in the frontal plane) and container length (in the sagittal plane). Four male subjects participated in the experiment. Their oxygen uptake and heart rate were monitored. Various strength measurements were made on the subjects before their participation in the study and after the study was over. The data were analyzed separately for lifting and lowering, using the analysis of variance. Container height did not affect the metabolic energy expenditure or heart rate of the subjects for lifting or lowering activities. Presence or absence of handles did not cause a significant change (alpha greater than or equal to .10) in the physiological responses for lowering tasks. All other task variable effects, however, were significant (alpha less than or equal to .01). A strong first and second order interaction was observed among the task variables. There was a significant increase in the strength (alpha less than or equal to .025) of subjects after performing manual materials handling activities for about seven weeks. A dimensional sensitivity analysis indicated that unit change in box width was more expensive than unit change in box length up to .56 m (22 inches). Beyond this, unit change in box length became critical. The weight/bulk ratio of the object did not affect the physiological responses.

The effects of speed, frequency, and load on measured hand forces for a floor to knuckle lifting task.

The purpose of this study was to describe and quantify measured hand forces during floor to knuckle lifting of various loads. Hand forces of five subjects were measured with a strain gauge apparatus for normal and fast speeds of lifting at 1, 4, and 8 l/min. The pattern of hand force over time exhibited peaks in force in the shape of a spike for all fast lifts, indicating that subjects did not lift smoothly. For normal speed of lift, only one of the five subjects executed some lifts smoothly, indicating that it may be possible to lift smoothly, but most lifters probably do not. Peaks of horizontal and vertical components of hand force were tabulated by speed of lift, frequency, and load.

Blood and component wastage report. A quality assurance function of the hospital transfusion committee.

The transfusion service of a regional referral medical center issues a monthly blood and component wastage report to the Hospital Transfusion Committee. The report includes the amount and type of units wasted, who is responsible, and the cost incurred by the wastage. The individuals responsible for wastage include physicians, nurses, and laboratory personnel. Physicians are responsible for most wastage, principally by failing to administer thawed or pooled blood products. The Hospital Transfusion Committee initiated a wastage-reduction program that included a letter to physicians indicating the patient's name and the type of blood and/or components that the addressed physicians was responsible for wasting. Simple corrective actions results in a 73 percent reduction in our blood and component wastage. Monitoring of blood and component wastage should be routine quality assurance function of the Hospital Transfusion Committee.

Assessment of the physical work capacity of institutionalized mentally retarded males.

Educable and trainable mentally retarded males (N=37) were examined for physical work capacity. Analysis of results indicated that the physical work capacity of the test population was 20 to 30% below that cited in the literature for nonretarded subjects of similar age and sex. Evidence also suggested that developmental and maintenance programs of physical fitness were required in order for mentally retarded persons to qualify for and maintain employment on most of the manual occupational tasks cited.

The psychophysical approach to manual materials handling task design.

For approximately three decades, researchers have utilized psychophysics to develop guidelines (weights, forces and frequencies) for manual materials handling tasks. Early work by Stover Snook and his colleagues provided the foundations of the experimental methodologies that would be used by other researchers as well as design data that would be used by practitioners. Currently, there are extensive psychophysical data for designing a variety of materials handling tasks. The current state of psychophysical data will be examined, and the psychophysical approach will be compared to the biomechanical and physiological approaches to setting limits for materials handling tasks. The advantages and disadvantages of the psychophysical approach will be discussed, as will the research needs required to address the current limitations of the psychophysical approach.

Work scheduling under normal and prolonged-vibration environments.

This paper summarizes the research conducted to study human performance and recovery under prolonged, whole-body, vertical vibration. The results showed that performance under vibratory environment is inferior to that under normal environment. Interaction between work-rest schedules and the vibratory environment was detected. Parameters affecting selection of optimum work-rest schedules are discussed.

Evaluation of the ability of power to predict low frequency lifting capacity.

An experiment was conducted to examine the role that maximal lifting power has in predicting maximum acceptable weight of lift (MAWL) for a frequency of one lift per 8 h. The secondary aim of the study was to compare the ability of power to predict MAWL to previously used measures of capacity including two measures of isometric strength, five measures of isokinetic strength, and isoinertial capacity on an incremental lifting test. Twenty-five male subjects volunteered to participate in the experiment. The isometric tests involved maximum voluntary contractions for composite lifting strength at vertical heights of 15 and 75 cm. Peak isokinetic strength was measured at velocities of 0.1, 0.2, 0.4, 0.6 and 0.8 m s-1 using a modified CYBEX II isokinetic dynamometer. Isoinertial lifting capacity was measured on the X-factor incremental lifting machine and peak power was measured on the incremental lifting machine by having subjects lift a 25 kg load as quickly as possible. The results indicate that peak isoinertial power is significantly correlated with MAWL, and this correlation was higher than any of the correlations between the other predictor variables and MAWL. The relationships between the isokinetic strength measures and MAWL were stronger than the relationships between the isometric measures and MAWL. Overall, the results suggest that tests used to predict MAWL should be dynamic rather than static.

Manual materials handling capabilities in non-standard postures.

Research efforts to establish manual materials handling (MMH) capabilities of individuals and populations have been conducted for many years. Most of the previous efforts have explored 'standard postures', utilizing two-handed, symmetric, sagittal plane MMH using unrestricted postures. Recognizing that many industrial MMH activities do not utilize 'standard postures', recent research projects have explored psychophysically determined MMH capacities in a variety of non-standard postures. Among the non-standard postures examined were: twisting while lifting or lowering, lifting and lowering from lying, sitting, kneeling, and squatting positions, and carrying loads under conditions of constricted ceiling heights. This paper presents the results of a series of previous research efforts at Texas Tech University. The results are presented in the form of population capabilities of both males and females for 99 MMH tasks using 'non-standard postures'. The data tables contain means and standard deviations of the data, as well as percentile distributions for the subject populations. Sample sizes for the experimental populations ranged from 45 to 50 subjects of each sex in the first three experiments to 20 subjects of each sex in the fourth set of experiments.

Psychophysical lifting capacity over extended periods.

The effect of time on an individual's lifting capacity over extended periods using a psychophysical approach was studied. Twelve male subjects estimated their lifting capacity in a 25 min period, and then attempted to lift this weight for an 8 h period under varying conditions. For one experimental condition the subjects were allowed to adjust the weight, the final adjusted maximum acceptable weight of lift (MAWOL) averaged 85.4% of the original MAWOL determined in the 25 min session. The subjects also attempted to lift for an 8 h period, without any weight adjustments. All 12 subjects lasted the 8 h at 2 lifts per min, but at a frequency of 8 lifts per min only three subjects completed the eight hour lifting task. This indicates that the psychophysical approach is a valid method to measure lifting capacity across the lower lifting frequency range but overestimates the lifting capacity at the higher frequency range. Slight fluctuations were noted in heart rate and oxygen consumption which were recorded every hour.