In vivo attenuation profile of 660 nm and 830 nm wavelengths on human elbow skin and calcaneus tendon of different phototypes.

Physical factors and tissue characteristics determine the transmission of light through tissues. One of the significant clinical limitations of photobiomodulation is the quantification of fluence delivered at application sites and optical penetration depth in vivo. There is also the difficulty of determining the distances of the application points to cover a uniformly irradiated area. Thus, the aim was to evaluate in vivo the influence of melanin on light transmission of the 660 nm and 830 nm laser wavelengths on skin and tendon. Thirty young individuals of both sexes were recruited, divided into two groups based on melanin index, and submitted to photobiomodulation protocols in the posterior region of the elbow (skin-skin) and the calcaneus tendon (skin-tendon-skin). The irradiation area was evaluated using a homemade linear array of five sensors. We found significant transmission power values for different melanin indexes and wavelengths (p<0.0001). Also, different equipment can generate significant differences in the transmitted power at an 830-nm wavelength. Average scattering values are 14 mm and 21 mm for 660 nm, in higher and lower melanin index, respectively. For 830 nm, values of 20 mm and 26 mm are indicated. Laser light transmission in vivo tissues is related to wavelength, beam diameter, tissue thickness, and composition, as well as melanin index. The 830-nm laser presents higher light transmission on the skin than 660 nm. The distances between the application points can be different, with higher values for 830 nm than 660 nm.

Arterial pulsation modulates the optical attenuation coefficient of skin.

Photoplethysmographic (PPG) signals arise from the modulation of light reflectivity on the skin due to changes of physiological origin. Imaging plethysmography (iPPG) is a video-based PPG method that can remotely monitor vital signs in a non-invasive manner. iPPG signals result from skin reflectivity modulation. The origin of such reflectivity modulation is still a subject of debate. Here, we have used optical coherence tomography (OCT) imaging to find whether iPPG signals may result from skin optical properties being directly or indirectly modulated by arterial transmural pressure propagation. The light intensity across the tissue was modeled through a simple exponential decay (Beer-Lambert law) to analyze in vivo the modulation of the optical attenuation coefficient of the skin by arterial pulsation. The OCT transversal images were acquired from a forearm of three subjects in a pilot study. The results show that the optical attenuation coefficient of skin changes at the same frequency as the arterial pulsation due to transmural pressure propagation (local ballistographic effect), but we cannot discard the contribution of global ballistographic effects.

Absorption and reduced scattering coefficient estimation in pigmented human skin tissue by experimental colorimetric fitting.

This study aims to estimate the optical properties, absorption (µ a), and reduced scattering (µ s ') coefficients of ex vivo human skin through the individual typology angle (ITA) by only using the skin color parameters. Human skin samples were grouped according to their ITA value and measured using a colorimeter for validation. An integrating sphere and the inverse adding-doubling algorithm were applied to compute the samples µ a and µ s '. The µ a increases as the ITA decreases. An axis swap was performed to generate the µ a versus the ITA for all wavelengths between 500 nm and 800 nm with a spectral resolution of 10 nm. Linearization was performed and a correlation was found. An equation to fit µ a based solely on the ITA values was estimated. The µ s ' does not change with ITA, but it could be fit with an inverse power law as a function of the wavelength. Both equations have a coefficient of determination (R 2) higher than 0.93, indicating a good agreement with our model. An experimental model to estimate the absorption and reduced scattering coefficients of ex vivo human skin through ITA was found. The model has high agreement with the experimental data, with an R 2 between 0.932 and 0.997, and these findings may be relevant for photobiomodulation and light treatment applications to estimate the effect of the melanin on the therapy.

Evaluating biochemical differences in thyroglobulin from normal and goiter tissues by infrared spectral imaging.

Thyroglobulin is a glycoiodoprotein that is produced by thyroid follicular cells; it is stored in follicles in structures known as colloids. The main function of this protein is to stock the hormones triiodothyronine (T3) and thyroxine (T4) until the body requires them. This study aims to demonstrate that infrared spectral imaging with appropriate multivariate analysis can reveal biochemical changes in this glycoprotein. The results achieved herein point out biochemical differences in the colloid samples obtained from normal and goiter patients including glycosylation and changes in the secondary conformational structure. We have presented the first spectral histopathology-based method to detect biochemical differences in thyroid colloids, such as TG iodination, glycosylation, and changes in the secondary structure in normal and goiter patients. The observed changes in the colloids were mainly due to the alterations in amide I and amide II (secondary conformation of proteins) and there is a correlation with different glycosylation between normal and goiter tissues.

Light transmission and bond strength of glass fiber posts submitted to different surface treatments.

STATEMENT OF PROBLEM: Light transmitted deep into the root canal is an important parameter to increase bonding of the cement to the post and dentin. Glass fiber posts seem to be an option to increase transmitted light, but literature on the light transmittance profile and power transmission to deep canal regions is lacking. PURPOSE: The purpose of this in vitro study was to evaluate light delivered by 2 types of fiberglass posts submitted to different surface treatments and to evaluate the bond strength. MATERIAL AND METHODS: Whiteposts and Superposts were allocated to 4 groups: no surface treatment, surface treatment with 24% hydrogen peroxide, surface treatment with silane, and surface treatment with 24% hydrogen peroxide plus silane. The total light transmitted by the posts was measured by using an integrating sphere to collect the diffuse light. The light profile that was laterally delivered to the post was measured with a power detector equipped with an optical fiber probe. The bond strength was measured with the push-out test. Scanning electron microscopy was used to evaluate the surface treatments. The light transmission data were analyzed by using a generalized linear model and the Bonferroni test and the bond strength values were evaluated by using ANOVA and the Tukey test (α=.05). RESULTS: The cervical third presented the highest transmission (74.1% for Whiteposts and 74.6% for Superposts), followed by the middle (20.9% for Whiteposts and 20.4% for Superposts) and apical (5.0% for both Whiteposts and Superposts) thirds. Superposts led to higher bond strength than Whiteposts (9.73 ±5.89 and 8.48 ±4.99 MPa, respectively). Surface treatment with silane and hydrogen peroxide plus silane afforded similar bond strength (11.4 ±6.4 and 10.7 ±5.6 MPa, respectively), which was higher as compared with the bond strength obtained after surface treatment with hydrogen peroxide. For both post types, the bond strength decreased from the cervical (12.2 ±6.0 MPa) to the middle (9.7 ±5.0 MPa) and apical (6.5 ±3.6 MPa) thirds. CONCLUSIONS: Light transmission and bond strength decreased from the cervical to the apical third. Surface treatment impacted bond strength; light transmission through Whiteposts was slightly higher than light transmission through Superposts.

Measurement of Physical Parameters and Development of a Light Emitting Diodes Device for Therapeutic Use.

INTRODUCTION: Effectiveness of light-emitting diode (LED) in biological tissue is due to the correct application of physical parameters. However, most studies found do not provide complete information on the physical characteristics of the diodes. It is necessary to carefully evaluate the diode parameters so that the results of research with this feature can be reproduced. The objective of this study was to develop a light-emitting device using LED, with proper measurements for application in clinical research. It was used 267 LEDs, powered with 12-V voltage and fixed on a plate of ethylene-vinyl acetate (25 × 42 cm), equidistant at 1.0 cm. For the calculation of red and infrared irradiation, a spectrometer was used, and the data were processed in routines implemented in the OriginPro 8.5.0 SR1 Software. The irradiance was determined by the integration of the spectral irradiation in the LED emission region. The red LED has a wavelength of 620 ± 10 nm, a power density of 52.86 mW/cm2, power of 6.6 mW, and total power of 1.76 W on the device. The infrared LED has a wavelength of 940 ± 10 nm, power density 33.7 mW/cm2, power of 6 mW, and total power of 1.6 W on the device. The LED characterization enables the generation and application of energy with greater precision and reproducibility. Besides, it is a light source, a device capable of framing large areas, reducing the time and cost of the application in different clinical conditions related to neuromuscular performance or rehabilitation.

Exposing Metarhizium acridum mycelium to visible light up-regulates a photolyase gene and increases photoreactivating ability.

Metarhizium acridum is an entomopathogen currently used against acridids. We have previously reported that exposing mycelium to visible light increases M. acridum tolerance to ultraviolet-B (UV-B) radiation. Here we evaluated if light could also increase tolerance to ultraviolet-C (UV-C) radiation. We observed that, as opposed to UV-B radiation, light did not increase tolerance to UV-C radiation under dark repair conditions. However, light did increase tolerance to UV-C radiation if photoreactivating light was present after UV-C exposure. Quantitative PCR experiments revealed that light up-regulates a photolyase gene. This is the first report showing that light regulates photoreactivating ability in M. acridum.

Variation on Molecular Structure, Crystallinity, and Optical Properties of Dentin Due to Nd:YAG Laser and Fluoride Aimed at Tooth Erosion Prevention.

This in vitro study evaluated the compositional, crystalline, and morphological effects promoted by Nd:YAG laser on root dentin, and verified the effects of laser and topical acidulated phosphate fluoride application (APF-gel) on dentin erosion. 180 bovine dentin slabs were randomized into 4 groups (n = 45): G1-untreated, G2-APF-gel (1.23% F-, 4 min), G3-Nd:YAG (1064 nm, 84.9 J/cm², 10 Hz), and G4-APF-gel application followed by Nd:YAG laser irradiation. The compositional, crystalline, and morphological effects promoted by treatments were investigated on five samples of each experimental group. The other samples were submitted to a 5-day, 10-day, or 15-day erosive and abrasive demineralization and remineralization cycling in order to create erosion lesions. The area and depth of lesions, as well as the optical attenuation coefficient, were assessed, and all data were statistically analysed (p < 0.05). Nd:YAG laser promoted the reduction of carbonate, the formation of tetracalcium phosphate, as well as the melting and recrystallization of the dentin surface. Laser significantly decreased the area and depth of erosion lesions and altered the optical attenuation coefficient when compared to untreated and APF-gel groups, but the association of APF-gel and laser did not promote an additional effect. Nd:YAG laser irradiation can be a promissory treatment to prevent dentin erosion and the abrasion process.

Digital de-waxing on FTIR images.

This paper presents a procedure that digitally neutralizes the contribution of paraffin to FTIR hyperspectral images. A brief mathematical derivation of the procedure is demonstrated and applied on one normal human colon sample to exemplify the de-waxing procedure. The proposed method includes construction of a paraffin model based on PCA, EMSC normalization and application of two techniques for spectral quality control. We discuss every step in which the researcher needs to take a subjective decision during the de-waxing procedure, and we explain how to make an adequate choice of parameters involved. Application of this procedure to 71 hyperspectral images collected from 55 human colon biopsies (20 normal, 17 ulcerative colitis, and 18 adenocarcinoma) showed that paraffin was appropriately neutralized, which made the de-waxed images adequate for analysis by pattern-recognition techniques such as k-means clustering or PCA-LDA.

Loss of structural water and carbonate of Nd:YAG laser-irradiated human enamel.

The objective of this study was to use Fourier Transform Infrared (FTIR) spectroscopy and Scanning Electron Microscopy (SEM) to assess whether Nd:YAG laser irradiation associated with a dye or not alters the chemical constitution of the enamel. Fourteen enamel sections were randomly divided into two groups: (1) Nd:YAG and (2) dye + Nd:YAG. First, the untreated enamel surfaces were analyzed by FTIR to acquire the control absorption spectrum. Next, Group 2 received a layer of inactivated coal diluted in deionized water before laser treatment. Enamel samples belonging to groups 1 and 2 were then irradiated with a 1,064-nm Nd:YAG laser (80 mJ, 10 Hz) in the contact mode; the carbonate absorption band and the water absorption band were measured in each sample after irradiation. The water band was measured again 24 h, 48 h, and 7 days after irradiation. Group 1 had statistically similar water and carbonate contents before and after irradiation. Group 2 displayed significantly lower (p < 0.05) water content after irradiation, which remained constant along time at 24 and 48 h. After 7 days, the water content increased slightly, being statistically higher than in the other experimental periods, except for the control. The carbonate/phosphate ratio was measured only at the beginning, and after irradiation, it decreased only in Group 2 indicating carbonate loss (p < 0.05). Irradiation with 1,064-nm Nd:YAG laser associated with a dye reduces the carbonate and structural water content in the enamel.

CO2 laser as auxiliary in the debonding of ceramic brackets.

This study evaluated the temperature in the bonding composite and in the pulp chamber, the shear bond strength after the irradiation of CO2 lasers, and the Adhesive Remnant Index (ARI) after debonding of ceramic bracket. A hundred and five premolars were used: 30 to evaluate the temperature and 75 to test the resistance to shear and the ARI. To assess the temperature, different irradiation times (3 and 5 s), pulse duration (0.001 and 0.003 s), and output power (5, 8, and 10 W) were tested (total of 12 groups). During all the irradiation, specimens were immersed in thermal bath water at 37 °C. In the test and ARI evaluation, premolars were divided into five groups (n = 15) and were submitted to the following regimens of CO2 laser irradiation: I (5 W, pulse duration = 0.01 s, application time = 3 s), II (5 W, 0.03 s, 3 s), III (8 W, 0.01 s, 3 s), and IV (1 0 W, 0.01 s, 3 s). Group C (control) was not subjected to irradiation. ARI was measured after debonding of the bracket. Following irradiation of the lasers, the pulpal temperature was not higher than 5.5 °C in four of the study groups. Results were submitted to the ANOVA and Duncan's test. CO2 laser irradiation regimen IV was one in which the strength of debonding is 7.33 MPa. Therefore, CO2 laser may aid removal of ceramic brackets; it decreased the bond strength without increasing the excessive temperature excessively.

Thermal effects and morphological aspects of varying Er:YAG laser energy on demineralized dentin removal: an in vitro study.

The aim of this study was to evaluate thermal changes, dentin ablation removal capacity, and morphological aspects of sound and demineralized human dentin surface irradiated with different output energies of an erbium: yttrium-aluminium-garnet (Er:YAG) laser. Eighty sound human tooth specimens were assigned into two groups: demineralized dentin and sound dentin (control group). The dentin groups were subdivided into four subgroups (n = 10) according to the irradiation energy used (120, 160, 200, or 250 mJ) at a constant frequency level of 6 Hz, in focused mode, and under refrigeration. Quantitative analysis of the sound and carious dentin ablation was performed using light microscopy (LM) by measuring (mm) the remaining demineralized tissue with the Axion Vision™ software. Qualitative analysis was performed using the images obtained with a scanning electron microscope (SEM), and the temperature increase was recorded with an infrared digital thermometer. The Er:YAG laser promoted a gradual increase in temperature for all groups, and no difference was observed between the sound and demineralized dentin. The groups of 200 and 250 mJ showed the highest values, yet a variation in temperature did not exceed 5 °C. The energy output of 120 mJ selectively removed demineralized tissue when compared to 250 mJ, while also providing more regular surfaces in the cavity preparation. It was concluded that the temperature increase during sound and demineralized dentin removal had a strong positive correlation with the Er:YAG laser energy level output. However, the higher energies used did not present selectivity to the demineralized tissue, and the parameters used did not cause an increase in temperature over 5 °C.

In vitro photodynamic inactivation of plant-pathogenic fungi Colletotrichum acutatum and Colletotrichum gloeosporioides with Novel Phenothiazinium photosensitizers.

The increasing tolerance to currently used fungicides in both clinical and agricultural areas is of great concern. The nonconventional light-based approach of antimicrobial photodynamic treatment (APDT) is a promising alternative to conventional fungicides. We evaluated the effects of APDT with four phenothiazinium derivatives (methylene blue [MB], new methylene blue N [NMBN], toluidine blue O [TBO], and the novel pentacyclic phenothiazinium photosensitizer [PS] S137) on conidia of three fungal species (Colletotrichum acutatum, Colletotrichum gloeosporioides, and Aspergillus nidulans). The efficacy of APDT with each PS was determined, initially, based on photosensitizer MICs. Additionally, the effects of APDT with two selected PSs (NMBN and S137) on survival of conidia were evaluated. The subcellular localization of the PS in C. acutatum conidia was determined. The effects of photodynamic treatments on leaves of the plant host Citrus sinensis were also investigated. APDT with S137 showed the lowest MIC. MICs for S137 were 5 µM for the three fungal species when a fluence of 25 J cm(-2) was used. APDT with NMBN (50 µM) and S137 (10 µM) resulted in a reduction in the survival of the conidia of all species of approximately 5 logs with fluences of ≥15 J cm(-2). Washing of the conidia before light exposure did not prevent photodynamic inactivation. Both NMBN and S137 accumulated in cytoplasmic structures, such as lipid bodies, of C. acutatum conidia. No damage to orange tree leaves was observed after APDT.

Deep neural networks can differentiate thyroid pathologies on infrared hyperspectral images.

BACKGROUND AND OBJECTIVE: The thyroid is a gland responsible for producing important body hormones. Several pathologies can affect this gland, such as thyroiditis, hypothyroidism, and thyroid cancer. The visual histological analysis of thyroid specimens is a valuable process that enables pathologists to detect diseases with high efficiency, providing the patient with a better prognosis. Existing computer vision systems developed to aid in the analysis of histological samples have limitations in distinguishing pathologies with similar characteristics or samples containing multiple diseases. To overcome this challenge, hyperspectral images are being studied to represent biological samples based on their molecular interaction with light. METHODS: In this study, we address the acquisition of infrared absorbance spectra from each voxel of histological specimens. This data is then used for the development of a multiclass fully-connected neural network model that discriminates spectral patterns, enabling the classification of voxels as healthy, cancerous, or goiter. RESULTS: Through experiments using the k-fold cross-validation protocol, we obtained an average accuracy of 93.66 %, a sensitivity of 93.47 %, and a specificity of 96.93 %. Our results demonstrate the feasibility of using infrared hyperspectral imaging to characterize healthy tissue and thyroid pathologies using absorbance measurements. The proposed deep learning model has the potential to improve diagnostic efficiency and enhance patient outcomes.

Histopathological diagnosis of colon cancer using micro-FTIR hyperspectral imaging and deep learning.

BACKGROUND AND OBJECTIVE: Current studies based on digital biopsy images have achieved satisfactory results in detecting colon cancer despite their limited visual spectral range. Such methods may be less accurate when applied to samples taken from the tumor margin region or to samples containing multiple diagnoses. In contrast with the traditional computer vision approach, micro-FTIR hyperspectral images quantify the tissue-light interaction on a histochemical level and characterize different tissue pathologies, as they present a unique spectral signature. Therefore, this paper investigates the possibility of using hyperspectral images acquired over micro-FTIR absorbance spectroscopy to characterize healthy, inflammatory, and tumor colon tissues. METHODS: The proposed method consists of modeling hyperspectral data into a voxel format to detect the patterns of each voxel using fully connected deep neural network. A web-based computer-aided diagnosis tool for inference is also provided. RESULTS: Our experiments were performed using the K-fold cross-validation protocol in an intrapatient approach and achieved an overall accuracy of 99% using a deep neural network and 96% using a linear support vector machine. Through the experiments, we noticed the high performance of the method in characterizing such tissues using deep learning and hyperspectral images, indicating that the infrared spectrum contains relevant information and can be used to assist pathologists during the diagnostic process.

An In Vitro Analysis of the Physical and Mechanical Behavior of a PEEK Component for an Implant-Supported and Retained Removable Dental Prosthesis.

PURPOSE: To investigate the physical and mechanical behaviors of polyether ether ketone (PEEK) before and after thermocycling, as well as its potential use as a more durable prosthetic component for implant-supported and -retained removable dental prostheses (I-RDPs). MATERIALS AND METHODS: Roughness and surface hardness were evaluated in specimens obtained using the subtractive method (n = 20) with a diameter of 9 mm and a thickness of 2 mm, and retention force was measured using attachments with a diameter of 4 mm and a height of 3 mm. For fatigue resistance testing, a polyurethane matrix with two ball abutment implants (MDL, Intra-Lock International) was used to simulate the mandibular alveolar ridge. A total of 40 attachments (n = 20 pairs) were placed in acrylic resin blocks using an analog technique for the direct clinical pickup of overdenture female attachments, then submitted to 2,900 insertion/removal cycles to simulate 24 months of overdenture use. Physical analyses were performed by Fourier-transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), and differential scanning calorimetry (DSC) before and after thermocycling (5°C to 55°C for 10,000 cycles). After normal distribution was verified by Shapiro-Wilk test, one-way ANOVA was applied to analyze the surface roughness and hardness, and two-way ANOVA with Bonferroni adjustment was used to assess the retention force (α = .05). RESULTS: Thermocycling did not change the PEEK surface roughness or hardness (P > .05). As for the retention force, the highest average was observed after the thermocycling test (P = .006). CONCLUSION: Based on the FTIR, XRD, and DSC results, PEEK crystallinity decreased after thermocycling, and the physical and mechanical behaviors of this polymer were compatible with the proposed application, suggesting that PEEK is a component of greater durability for I-RDPs. Int J Prosthodont 2023;36:612-619.

Optical Properties of Human Skin Phototypes and Their Correlation with Individual Angle Typology.

Objective: This study aims to correlate human skin phototypes with complete optical characterization (absorption, scattering, effective attenuation, optical penetration, and albedo coefficients) based on individual typology angle (ITA) values and colorimetric parameters. Methods: A colorimeter was used to group 12, fresh, ex vivo human skin samples according to their phototype; the CIELAB color scale and ITA values were employed. An integrating sphere system and the inverse adding-doubling algorithm were applied during optical characterization, conducted from 500 to 1300 nm. Results: On the basis of ITA values and their classification, the skin samples were separated into six groups: two intermediates, two tan, and two brown. In the visible range, for lower ITA values (darker skins), the absorption and effective attenuation coefficient parameters increased, whereas the albedo and depth penetration parameters decreased. In the infrared region, all the phototypes had similar parameters. The scattering coefficient was similar for all the samples and did not change with ITA values. Conclusions: ITA analysis, a quantitative method, showed that the human skin tissue's optical properties and pigmentation colors were highly correlated.

Mitigating skin tone bias in linear array in vivo photoacoustic imaging with short-lag spatial coherence beamforming.

Photoacoustic (PA) imaging has the potential to deliver non-invasive diagnostic information. However, skin tone differences bias PA target visualization, as the elevated optical absorption of melanated skin decreases optical fluence within the imaging plane and increases the presence of acoustic clutter. This paper demonstrates that short-lag spatial coherence (SLSC) beamforming mitigates this bias. PA data from the forearm of 18 volunteers were acquired with 750-, 810-, and 870-nm wavelengths. Skin tones ranging from light to dark were objectively quantified using the individual typology angle (ITA°). The signal-to-noise ratio (SNR) of the radial artery (RA) and surrounding clutter were measured. Clutter was minimal (e.g., -16 dB relative to the RA) with lighter skin tones and increased to -8 dB with darker tones, which compromised RA visualization in conventional PA images. SLSC beamforming achieved a median SNR improvement of 3.8 dB, resulting in better RA visualization for all skin tones.

Temperature rise during Er:YAG cavity preparation of primary enamel.

This study aimed to assess in vitro thermal alterations taking place during the Er:YAG laser cavity preparation of primary tooth enamel at different energies and pulse repetition rates. Forty healthy human primary molars were bisected in a mesio-distal direction, thus providing 80 fragments. Two small orifices were made on the dentin surface to which type K thermocouples were attached. The fragments were individually fixed with wax in a cylindrical Plexiglass® abutment and randomly assigned to eight groups, according to the laser parameters (n = 10): G1 - 250 mJ/ 3 Hz, G2 - 250 mJ/ 4 Hz, G3 - 250 mJ/ 6 Hz, G4 - 250 mJ/10 Hz, G5 - 250 mJ/ 15 Hz, G6 - 300 mJ/ 3 Hz, G7 - 300 mJ/ 4 Hz and G8 - 300 mJ/ 6 Hz. An area of 4 mm(2) was delimited. Cavities were done (2 mm long × 2 mm wide × 1 mm thick) using non-contact (12 mm) and focused mode. Temperature values were registered from the start of laser irradiation until the end of cavity preparation. Data were analyzed by one-way ANOVA and Tukey test (p ≤ 0.05). Groups G1, G2, G6, and G7 were statistically similar and furnished the lowest mean values of temperature rise. The set 250 mJ/10 and 15 Hz yielded the highest temperature values. The sets 250 and 300 mJ and 6 Hz provided temperatures with mean values below the acceptable critical value, suggesting that these parameters ablate the primary tooth enamel. Moreover, the temperature elevation was directly related to the increase in the employed pulse repetition rates. In addition, there was no direct correlation between temperature rise and energy density. Therefore, it is important to use a lower pulse frequency, such as 300 mJ and 6 Hz, during cavity preparation in pediatric patients.

Laser light sources for photobiomodulation: The role of power and beam characterization in treatment accuracy and reliability.

PURPOSE: Daily clinical use of therapeutic light sources can lead to changes in light emission stability with potentially significant consequences for usage in photomedicine treatment. The aim of this study was to evaluate the average and maximum power and to describe the beam diameter of different low-power laser photobiomodulation devices in clinical use in Brazil. METHODS: The power and light-emitting beam diameter of twenty-four therapeutic devices with an average age of 11±5 years, with an average weekly use of fewer than thirty minutes, were measured. RESULTS: The analyzed power varied between 2% to 134% of the values declared by the manufacturers. Differences in beam diameter of between 38% and 543% of the nominal values were also observed. It is also noteworthy that even between the same brand and model, differences in diameter were obtained. Finally, differences were observed in the power output after one and three minutes of sequential emission for 830 nm and 904 nm (p < 0.05), but not when comparing the difference between wavelengths in factor time. CONCLUSION: There is a need for a shared effort on the part of laser manufacturers to improve standardization and consistency of laser output power and beam diameters. At the same time, medical laser operators should also consider development of standardized protocols for maintenance and monitoring equipment performance over time to correct for fluctuations that could ultimately impact on treatment outcomes.