Introducing the GRACEnet/REAP Data Contribution, Discovery, and Retrieval System.

Difficulties in accessing high-quality data on trace gas fluxes and performance of bioenergy/bioproduct feedstocks limit the ability of researchers and others to address environmental impacts of agriculture and the potential to produce feedstocks. To address those needs, the GRACEnet (Greenhouse gas Reduction through Agricultural Carbon Enhancement network) and REAP (Renewable Energy Assessment Project) research programs were initiated by the USDA Agricultural Research Service (ARS). A major product of these programs is the creation of a database with greenhouse gas fluxes, soil carbon stocks, biomass yield, nutrient, and energy characteristics, and input data for modeling cropped and grazed systems. The data include site descriptors (e.g., weather, soil class, spatial attributes), experimental design (e.g., factors manipulated, measurements performed, plot layouts), management information (e.g., planting and harvesting schedules, fertilizer types and amounts, biomass harvested, grazing intensity), and measurements (e.g., soil C and N stocks, plant biomass amount and chemical composition). To promote standardization of data and ensure that experiments were fully described, sampling protocols and a spreadsheet-based data-entry template were developed. Data were first uploaded to a temporary database for checking and then were uploaded to the central database. A Web-accessible application allows for registered users to query and download data including measurement protocols. Separate portals have been provided for each project (GRACEnet and REAP) at nrrc.ars.usda.gov/slgracenet/#/Home and nrrc.ars.usda.gov/slreap/#/Home. The database architecture and data entry template have proven flexible and robust for describing a wide range of field experiments and thus appear suitable for other natural resource research projects.

Peer relationships as a source of support for adolescent mothers.

PIP: There is speculation that the pregnancy and parenthood of adolescent mothers cause changes in their peer relationships such that their peers become less able to provide support. Little empirical evidence exists to support such speculation. The authors assessed the relative amounts and types of support provided by friends and relatives to adolescent mothers, and examined peer support in relation to parenting stress. Invitations were sent to all 480 women recruited through public access birth records in two northeastern Ohio counties who were 19 years old and younger and who had given birth to their first child during the previous nine months. The counties incorporate urban, suburban, and rural communities. 66 women volunteered, of whom 46 eventually participated in the study. They were aged 13-19 years (mean age, 17.43 years) at the birth of their child. Approximately 66% were White and 12 were married. Of the 36 women who reported their family income, 88% reported yearly income of less than $20,000; of those, 15 reported income of less than $5000. 28 of the adolescent mothers were unemployed. 22 were enrolled in school at the time of data collection. Of the 24 who were not enrolled in school, 15 had already completed 12th grade. The Inventory of Social Contacts measured levels of perceived child-rearing, emotional, and material support and interference from family and friends, while the Parenting Stress Index assessed self-reported parenting stress arising from child and parent characteristics. Results of repeated-measures ANOVAs and Pearson correlations indicate that, compared to family, friends provide more emotional support and less interference. Parenting stress is buffered more effectively by the support provided by friends.^ieng

Stabilization of chimeric BR96-doxorubicin immunoconjugate.

Chimeric BR96-doxorubicin conjugate (BR96-DOX) is an immunoconjugate designed to specifically target and kill certain tumor cells. The linker between the chimeric BR96 antibody and DOX is an acid-labile hydrazone group which was designed to undergo lysosomal hydrolysis to release DOX in vivo. Stability studies indicated that acid-catalyzed hydrazone hydrolysis was the major degradation route in vitro. Even under optimal conditions of pH and temperature, the stability of BR96-DOX in solution was not acceptable for long-term storage. Lyophilization of BR96-DOX in the presence of added sugars, such as lactose or sucrose, and subsequent storage of the lyophile under refrigeration significantly improved the stability. Therefore lyophilization appears to be a viable approach for achieving long-term stabilization of BR96-DOX.