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1.
Behav Res Methods ; 2023 Dec 29.
Artículo en Inglés | MEDLINE | ID: mdl-38158551

RESUMEN

Formants (vocal tract resonances) are increasingly analyzed not only by phoneticians in speech but also by behavioral scientists studying diverse phenomena such as acoustic size exaggeration and articulatory abilities of non-human animals. This often involves estimating vocal tract length acoustically and producing scale-invariant representations of formant patterns. We present a theoretical framework and practical tools for carrying out this work, including open-source software solutions included in R packages soundgen and phonTools. Automatic formant measurement with linear predictive coding is error-prone, but formant_app provides an integrated environment for formant annotation and correction with visual and auditory feedback. Once measured, formants can be normalized using a single recording (intrinsic methods) or multiple recordings from the same individual (extrinsic methods). Intrinsic speaker normalization can be as simple as taking formant ratios and calculating the geometric mean as a measure of overall scale. The regression method implemented in the function estimateVTL calculates the apparent vocal tract length assuming a single-tube model, while its residuals provide a scale-invariant vowel space based on how far each formant deviates from equal spacing (the schwa function). Extrinsic speaker normalization provides more accurate estimates of speaker- and vowel-specific scale factors by pooling information across recordings with simple averaging or mixed models, which we illustrate with example datasets and R code. The take-home messages are to record several calls or vowels per individual, measure at least three or four formants, check formant measurements manually, treat uncertain values as missing, and use the statistical tools best suited to each modeling context.

2.
J Acoust Soc Am ; 150(5): 3949, 2021 11.
Artículo en Inglés | MEDLINE | ID: mdl-34852594

RESUMEN

To investigate the perception of gender from children's voices, adult listeners were presented with /hVd/ syllables, in isolation and in sentence context, produced by children between 5 and 18 years. Half the listeners were informed of the age of the talker during trials, while the other half were not. Correct gender identifications increased with talker age; however, performance was above chance even for age groups where the cues most often associated with gender differentiation (i.e., average fundamental frequency and formant frequencies) were not consistently different between boys and girls. The results of acoustic models suggest that cues were used in an age-dependent manner, whether listeners were explicitly told the age of the talker or not. Overall, results are consistent with the hypothesis that talker age and gender are estimated jointly in the process of speech perception. Furthermore, results show that the gender of individual talkers can be identified accurately well before reliable anatomical differences arise in the vocal tracts of females and males. In general, results support the notion that the transmission of gender information from voice depends substantially on gender-dependent patterns of articulation, rather than following deterministically from anatomical differences between male and female talkers.


Asunto(s)
Percepción del Habla , Voz , Acústica , Adulto , Niño , Señales (Psicología) , Femenino , Humanos , Masculino , Acústica del Lenguaje
3.
J Acoust Soc Am ; 147(3): EL271, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32237846

RESUMEN

Listeners show better-than-chance discrimination of nasalized and oral vowels occurring in appropriate consonantal contexts. Yet, the methods for investigating partial perceptual compensation for nasal coarticulation often include nasal and oral vowels containing naturally different pitch contours. Listeners may therefore be discriminating between these vowels based on pitch differences and not nasalization. The current study investigates the effect of pitch variation on the discrimination of nasalized and oral vowels in C_N and C_C items. The f0 contour of vowels within paired discrimination trials was varied. The results indicate that pitch variation does not influence patterns of partial perceptual compensation for coarticulation.

4.
J Acoust Soc Am ; 143(2): EL61, 2018 02.
Artículo en Inglés | MEDLINE | ID: mdl-29495730

RESUMEN

Apparent-talker height is determined by a talker's fundamental frequency (f0) and spectral information, typically indexed using formant frequencies (FFs). Barreda [(2017b). J. Acoust. Soc. Am. 141, 4781-4792] reports that the apparent height of a talker can be influenced by vowel-specific variation in the f0 or FFs of a sound. In this experiment, native speakers of Mandarin were presented with a series of syllables produced by talkers of different apparent heights. Results indicate that there is substantial variability in the estimated height of a single talker based on lexical tone, as well as the inherent f0 and FFs of vowel phonemes.

5.
J Acoust Soc Am ; 144(1): 500, 2018 07.
Artículo en Inglés | MEDLINE | ID: mdl-30075677

RESUMEN

Researchers investigating the vowel systems of languages or dialects frequently employ normalization methods to minimize between-speaker variability in formant patterns while preserving between-phoneme separation and (socio-)dialectal variation. Here two methods are considered: log-mean and Lobanov normalization. Although both of these methods express formants in a speaker-dependent space, the methods differ in their complexity and in their implied models of human vowel-perception. Typical implementations of these methods rely on balanced data across speakers so that researchers may have to reduce the data available in the analyses in missing-data situations. Here, an alternative method is proposed for the normalization of vowels using the log-mean method in a linear-regression framework. The performance of the traditional approaches to log-mean and Lobanov normalization against the regression approach to the log-mean method using naturalistic, simulated vowel-data was investigated. The results indicate that the Lobanov method likely removes legitimate linguistic variation from vowel data and often provides very noisy estimates of the actual vowel quality associated with individual tokens. The authors further argue that the Lobanov method is too complex to represent a plausible model of human vowel perception, and so is unlikely to provide results that reflect the true perceptual organization of linguistic data.


Asunto(s)
Análisis de Datos , Fonética , Acústica del Lenguaje , Percepción del Habla/fisiología , Humanos , Lenguaje
6.
J Acoust Soc Am ; 143(5): EL361, 2018 05.
Artículo en Inglés | MEDLINE | ID: mdl-29857760

RESUMEN

Adult listeners were presented with /hVd/ syllables spoken by boys and girls ranging from 5 to 18 years of age. Half of the listeners were informed of the sex of the speaker; the other half were not. Results indicate that veridical age in children can be predicted accurately based on the acoustic characteristics of the talker's voice and that listener behavior is highly predictable on the basis of speech acoustics. Furthermore, listeners appear to incorporate assumptions about talker sex into their estimates of talker age, even when information about the talker's sex is not explicitly provided for them.


Asunto(s)
Estimulación Acústica/métodos , Acústica del Lenguaje , Percepción del Habla/fisiología , Adolescente , Factores de Edad , Niño , Preescolar , Femenino , Humanos , Masculino , Factores Sexuales
7.
J Acoust Soc Am ; 141(6): 4781, 2017 06.
Artículo en Inglés | MEDLINE | ID: mdl-28679275

RESUMEN

The perception of apparent-talker height is mostly determined by the fundamental frequency (f0) and spectral characteristics of a voice. Although it is traditionally thought that spectral cues affect apparent-talker height by influencing apparent vocal-tract length, a recent experiment [Barreda (2016). J. Phon. 55, 1-18] suggests that apparent-talker height can vary significantly within-talker on the basis of phonemically-determined spectral variability. In this experiment, listeners were asked to estimate the height of 10 female talkers based on manipulated natural productions of bVd words containing one of /i æ ɑ u ɝ/. Results indicate that although listeners appear to use vocal-tract length estimates in determining apparent-height, apparent-talker height also varies significantly within-talker based on the inherent spectral and source characteristics of different vowels, with vowels with lower formant-frequencies and f0 being associated with taller talkers overall. The use of spectral and f0 information in apparent-height estimation varied considerably between listeners, resulting in additional variation in the apparent-height of talkers. Although the use of acoustic information in the determination of apparent-height was highly systematic, it does not necessarily follow from the empirical relationship between speech acoustics and actual talker height.

8.
Sci Rep ; 14(1): 15611, 2024 Jul 06.
Artículo en Inglés | MEDLINE | ID: mdl-38971806

RESUMEN

This study compares how English-speaking adults and children from the United States adapt their speech when talking to a real person and a smart speaker (Amazon Alexa) in a psycholinguistic experiment. Overall, participants produced more effortful speech when talking to a device (longer duration and higher pitch). These differences also varied by age: children produced even higher pitch in device-directed speech, suggesting a stronger expectation to be misunderstood by the system. In support of this, we see that after a staged recognition error by the device, children increased pitch even more. Furthermore, both adults and children displayed the same degree of variation in their responses for whether "Alexa seems like a real person or not", further indicating that children's conceptualization of the system's competence shaped their register adjustments, rather than an increased anthropomorphism response. This work speaks to models on the mechanisms underlying speech production, and human-computer interaction frameworks, providing support for routinized theories of spoken interaction with technology.


Asunto(s)
Habla , Humanos , Adulto , Niño , Masculino , Femenino , Habla/fisiología , Adulto Joven , Adolescente , Psicolingüística
9.
J Acoust Soc Am ; 133(2): 1065-77, 2013 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-23363122

RESUMEN

The vocal tract length of a speaker is the primary determinant of the range of formant frequencies (FFs) produced by that speaker. Listeners have demonstrated sensitivity to the average FFs produced by voices, for example, in estimating the relative heights of two speakers based on their speech. However, it is not known whether they can learn to identify voices based on the acoustic characteristic associated with the average FFs produced by a voice (this characteristic will be referred to as FF-scaling). To investigate this, a series of vowels corresponding to voices that differed in their average f0 and/or FF-scaling were synthesized. Listeners (n = 71) were trained to identify these voices using a training procedure where, for each trial, they heard the vowels representing a voice and then had to identify the stimulus voice from among a series of candidate voices that differed in terms of their FF-scaling and/or their f0. Results indicate that listeners can identify voices on the basis of FF-scaling quite accurately and consistently after only a short training session and that, although f0 weakly influences these estimates, they are most strongly determined by the stimulus FFs.


Asunto(s)
Aprendizaje Discriminativo , Discriminación en Psicología , Acústica del Lenguaje , Percepción del Habla , Calidad de la Voz , Estimulación Acústica , Atención , Audiometría del Habla , Estudios de Factibilidad , Humanos , Juicio , Psicoacústica , Reconocimiento en Psicología , Análisis y Desempeño de Tareas , Factores de Tiempo
10.
J Speech Lang Hear Res ; 66(2): 545-564, 2023 02 13.
Artículo en Inglés | MEDLINE | ID: mdl-36729698

RESUMEN

PURPOSE: This study investigates the debate that musicians have an advantage in speech-in-noise perception from years of targeted auditory training. We also consider the effect of age on any such advantage, comparing musicians and nonmusicians (age range: 18-66 years), all of whom had normal hearing. We manipulate the degree of fundamental frequency (f o) separation between the competing talkers, as well as use different tasks, to probe attentional differences that might shape a musician's advantage across ages. METHOD: Participants (ranging in age from 18 to 66 years) included 29 musicians and 26 nonmusicians. They completed two tasks varying in attentional demands: (a) a selective attention task where listeners identify the target sentence presented with a one-talker interferer (Experiment 1), and (b) a divided attention task where listeners hear two vowels played simultaneously and identify both competing vowels (Experiment 2). In both paradigms, f o separation was manipulated between the two voices (Δf o = 0, 0.156, 0.306, 1, 2, 3 semitones). RESULTS: Results show that increasing differences in f o separation lead to higher accuracy on both tasks. Additionally, we find evidence for a musician's advantage across the two studies. In the sentence identification task, younger adult musicians show higher accuracy overall, as well as a stronger reliance on f o separation. Yet, this advantage declines with musicians' age. In the double vowel identification task, musicians of all ages show an across-the-board advantage in detecting two vowels-and use f o separation more to aid in stream separation-but show no consistent difference in double vowel identification. CONCLUSIONS: Overall, we find support for a hybrid auditory encoding-attention account of music-to-speech transfer. The musician's advantage includes f o, but the benefit also depends on the attentional demands in the task and listeners' age. Taken together, this study suggests a complex relationship between age, musical experience, and speech-in-speech paradigm on a musician's advantage. SUPPLEMENTAL MATERIAL: https://doi.org/10.23641/asha.21956777.


Asunto(s)
Música , Percepción del Habla , Adulto , Humanos , Adolescente , Adulto Joven , Persona de Mediana Edad , Anciano , Habla , Audición , Ruido , Atención
11.
J Acoust Soc Am ; 132(5): 3453-64, 2012 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-23145625

RESUMEN

Many experiments have reported a perceptual advantage for vowels presented in blocked-versus mixed-voice conditions. Nusbaum and colleagues [Nusbaum and Morin (1992). in Speech Perception, Speech Production, and Linguistic Structure, edited by Y. Tohkura, Y. Sagisaka, and E. Vatikiotis-Bateson (OHM, Tokyo), pp. 113-134; Magnuson and Nusbaum (2007). J. Exp. Psychol. Hum. Percept. Perform. 33(2), 391-409] present results which suggest that the size of this advantage may be related to the facility with which listeners can detect speaker changes, so that combinations of less similar voices can result in better performance than combinations of more similar voices. To test this, a series of synthetic voices (differing in their source characteristics and/or formant-spaces) was used in a speeded-monitoring task. Vowels were presented in blocks made up of tokens from one or two synthetic voices. Results indicate that formant-space differences, in the absence of source differences between voices in a block, were unlikely to result in the perception of multiple voices, leading to lower accuracy and relatively faster reaction times. Source differences between voices in a block resulted in the perception of multiple voices, increased reaction times, and a decreased negative effect of formant-space differences between voices on identification accuracy. These results are consistent with a process in which the detection of speaker changes guides the appropriate or inappropriate use of extrinsic information in normalization.


Asunto(s)
Acústica del Lenguaje , Percepción del Habla , Calidad de la Voz , Estimulación Acústica , Audiometría del Habla , Humanos , Enmascaramiento Perceptual , Tiempo de Reacción , Reconocimiento en Psicología , Detección de Señal Psicológica , Factores de Tiempo
12.
J Acoust Soc Am ; 131(1): 466-77, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22280608

RESUMEN

Several experiments have found that changing the intrinsic f0 of a vowel can have an effect on perceived vowel quality. It has been suggested that these shifts may occur because f0 is involved in the specification of vowel quality in the same way as the formant frequencies. Another possibility is that f0 affects vowel quality indirectly, by changing a listener's assumptions about characteristics of a speaker who is likely to have uttered the vowel. In the experiment outlined here, participants were asked to listen to vowels differing in terms of f0 and their formant frequencies and report vowel quality and the apparent speaker's gender and size on a trial-by-trial basis. The results presented here suggest that f0 affects vowel quality mainly indirectly via its effects on the apparent-speaker characteristics; however, f0 may also have some residual direct effects on vowel quality. Furthermore, the formant frequencies were also found to have significant indirect effects on vowel quality by way of their strong influence on the apparent speaker.


Asunto(s)
Fonética , Percepción del Habla/fisiología , Estimulación Acústica/métodos , Adolescente , Adulto , Femenino , Humanos , Juicio/fisiología , Masculino , Persona de Mediana Edad , Reconocimiento en Psicología/fisiología , Adulto Joven
13.
Sci Rep ; 11(1): 12110, 2021 06 08.
Artículo en Inglés | MEDLINE | ID: mdl-34103573

RESUMEN

Wearing surgical masks or other similar face coverings can reduce the emission of expiratory particles produced via breathing, talking, coughing, or sneezing. Although it is well established that some fraction of the expiratory airflow leaks around the edges of the mask, it is unclear how these leakage airflows affect the overall efficiency with which masks block emission of expiratory aerosol particles. Here, we show experimentally that the aerosol particle concentrations in the leakage airflows around a surgical mask are reduced compared to no mask wearing, with the magnitude of reduction dependent on the direction of escape (out the top, the sides, or the bottom). Because the actual leakage flowrate in each direction is difficult to measure, we use a Monte Carlo approach to estimate flow-corrected particle emission rates for particles having diameters in the range 0.5-20 µm. in all orientations. From these, we derive a flow-weighted overall number-based particle removal efficiency for the mask. The overall mask efficiency, accounting both for air that passes through the mask and for leakage flows, is reduced compared to the through-mask filtration efficiency, from 93 to 70% for talking, but from only 94-90% for coughing. These results demonstrate that leakage flows due to imperfect sealing do decrease mask efficiencies for reducing emission of expiratory particles, but even with such leakage surgical masks provide substantial control.


Asunto(s)
Aerosoles , Control de Enfermedades Transmisibles/métodos , Tos , Espiración , Filtración , Máscaras , Virosis/prevención & control , Adolescente , Adulto , COVID-19/prevención & control , Falla de Equipo , Femenino , Humanos , Masculino , Persona de Mediana Edad , Método de Montecarlo , Tamaño de la Partícula , Probabilidad , Respiración , Estornudo , Adulto Joven
14.
Sci Rep ; 10(1): 15665, 2020 09 24.
Artículo en Inglés | MEDLINE | ID: mdl-32973285

RESUMEN

The COVID-19 pandemic triggered a surge in demand for facemasks to protect against disease transmission. In response to shortages, many public health authorities have recommended homemade masks as acceptable alternatives to surgical masks and N95 respirators. Although mask wearing is intended, in part, to protect others from exhaled, virus-containing particles, few studies have examined particle emission by mask-wearers into the surrounding air. Here, we measured outward emissions of micron-scale aerosol particles by healthy humans performing various expiratory activities while wearing different types of medical-grade or homemade masks. Both surgical masks and unvented KN95 respirators, even without fit-testing, reduce the outward particle emission rates by 90% and 74% on average during speaking and coughing, respectively, compared to wearing no mask, corroborating their effectiveness at reducing outward emission. These masks similarly decreased the outward particle emission of a coughing superemitter, who for unclear reasons emitted up to two orders of magnitude more expiratory particles via coughing than average. In contrast, shedding of non-expiratory micron-scale particulates from friable cellulosic fibers in homemade cotton-fabric masks confounded explicit determination of their efficacy at reducing expiratory particle emission. Audio analysis of the speech and coughing intensity confirmed that people speak more loudly, but do not cough more loudly, when wearing a mask. Further work is needed to establish the efficacy of cloth masks at blocking expiratory particles for speech and coughing at varied intensity and to assess whether virus-contaminated fabrics can generate aerosolized fomites, but the results strongly corroborate the efficacy of medical-grade masks and highlight the importance of regular washing of homemade masks.


Asunto(s)
Infecciones por Coronavirus/prevención & control , Infecciones por Coronavirus/transmisión , Máscaras , Pandemias/prevención & control , Neumonía Viral/prevención & control , Neumonía Viral/transmisión , Prevención Primaria/métodos , Dispositivos de Protección Respiratoria , Adolescente , Adulto , Aerosoles , Betacoronavirus , COVID-19 , Tos/virología , Espiración , Femenino , Filtración/instrumentación , Humanos , Exposición por Inhalación , Masculino , Persona de Mediana Edad , Prevención Primaria/instrumentación , SARS-CoV-2 , Adulto Joven
15.
PLoS One ; 15(1): e0227699, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31986165

RESUMEN

Previously, we demonstrated a strong correlation between the amplitude of human speech and the emission rate of micron-scale expiratory aerosol particles, which are believed to play a role in respiratory disease transmission. To further those findings, here we systematically investigate the effect of different 'phones' (the basic sound units of speech) on the emission of particles from the human respiratory tract during speech. We measured the respiratory particle emission rates of 56 healthy human volunteers voicing specific phones, both in isolation and in the context of a standard spoken text. We found that certain phones are associated with significantly higher particle production; for example, the vowel /i/ ("need," "sea") produces more particles than /ɑ/ ("saw," "hot") or /u/ ("blue," "mood"), while disyllabic words including voiced plosive consonants (e.g., /d/, /b/, /g/) yield more particles than words with voiceless fricatives (e.g., /s/, /h/, /f/). These trends for discrete phones and words were corroborated by the time-resolved particle emission rates as volunteers read aloud from a standard text passage that incorporates a broad range of the phones present in spoken English. Our measurements showed that particle emission rates were positively correlated with the vowel content of a phrase; conversely, particle emission decreased during phrases with a high fraction of voiceless fricatives. Our particle emission data is broadly consistent with prior measurements of the egressive airflow rate associated with the vocalization of various phones that differ in voicing and articulation. These results suggest that airborne transmission of respiratory pathogens via speech aerosol particles could be modulated by specific phonetic characteristics of the language spoken by a given human population, along with other, more frequently considered epidemiological variables.


Asunto(s)
Espiración/fisiología , Infecciones del Sistema Respiratorio/transmisión , Habla/fisiología , Adolescente , Adulto , Aerosoles , Microbiología del Aire , Tos/microbiología , Tos/fisiopatología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Fonética , Infecciones del Sistema Respiratorio/microbiología , Acústica del Lenguaje , Pruebas de Articulación del Habla , Voz/fisiología , Adulto Joven
16.
Sci Rep ; 9(1): 2348, 2019 02 20.
Artículo en Inglés | MEDLINE | ID: mdl-30787335

RESUMEN

Mechanistic hypotheses about airborne infectious disease transmission have traditionally emphasized the role of coughing and sneezing, which are dramatic expiratory events that yield both easily visible droplets and large quantities of particles too small to see by eye. Nonetheless, it has long been known that normal speech also yields large quantities of particles that are too small to see by eye, but are large enough to carry a variety of communicable respiratory pathogens. Here we show that the rate of particle emission during normal human speech is positively correlated with the loudness (amplitude) of vocalization, ranging from approximately 1 to 50 particles per second (0.06 to 3 particles per cm3) for low to high amplitudes, regardless of the language spoken (English, Spanish, Mandarin, or Arabic). Furthermore, a small fraction of individuals behaves as "speech superemitters," consistently releasing an order of magnitude more particles than their peers. Our data demonstrate that the phenomenon of speech superemission cannot be fully explained either by the phonic structures or the amplitude of the speech. These results suggest that other unknown physiological factors, varying dramatically among individuals, could affect the probability of respiratory infectious disease transmission, and also help explain the existence of superspreaders who are disproportionately responsible for outbreaks of airborne infectious disease.


Asunto(s)
Aerosoles/análisis , Transmisión de Enfermedad Infecciosa/prevención & control , Espiración/fisiología , Habla/fisiología , Adulto , Tos/fisiopatología , Femenino , Humanos , Percepción Sonora/fisiología , Masculino , Tamaño de la Partícula , Estornudo/fisiología
17.
J Appl Physiol (1985) ; 121(3): 615-22, 2016 09 01.
Artículo en Inglés | MEDLINE | ID: mdl-27402557

RESUMEN

We evaluated genioglossus (GG) gross motoneuron morphology, electromyographic (EMG) activities, and respiratory patterning in rat pups allowed to develop without interference (unexposed) and pups born to dams subjected to osmotic minipump implantation in utero (saline-exposed). In experiment 1, 48 Sprague-Dawley rat pups (Charles-River Laboratories), ages postnatal day 7 (P7) through postnatal day 10 (P10), were drawn from two experimental groups, saline-exposed (n = 24) and unexposed (n = 24), and studied on P7, P8, P9, or P10. Pups in both groups were sedated (Inactin hydrate, 70 mg/kg), and fine-wire electrodes were inserted into the GG muscle of the tongue and intercostal muscles to record EMG activities during breathing in air and at three levels of normoxic hypercapnia [inspired CO2 fraction (FiCO2 ): 0.03, 0.06, and 0.09]. Using this approach, we assessed breathing frequency, heart rate, apnea type, respiratory event types, and respiratory stability. In experiment 2, 16 rat pups were drawn from the same experimental groups, saline-exposed (n = 9) and unexposed (n = 7), and used in motoneuron-labeling studies. In these pups a retrograde dye was injected into the GG muscle, and the brain stems were subsequently harvested and sliced. Labeled GG motoneurons were identified with microscopy, impaled, and filled with Lucifer yellow. Double-labeled motoneurons were reconstructed, and the number of primary projections and soma volumes were calculated. Whereas pups in each group exhibited the same number (P = 0.226) and duration (P = 0.093) of respiratory event types and comparable motoneuron morphologies, pups in the implant group exhibited more central apneas and respiratory instability relative to pups allowed to develop without interference.


Asunto(s)
Bombas de Infusión Implantables , Contracción Muscular/fisiología , Músculo Esquelético/fisiología , Mecánica Respiratoria/fisiología , Lengua/fisiología , Animales , Femenino , Humanos , Recién Nacido , Masculino , Miniaturización , Implantación de Prótesis , Ratas , Ratas Sprague-Dawley
18.
Respir Physiol Neurobiol ; 208: 45-50, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25596542

RESUMEN

Neonates at risk for sudden infant death syndrome (SIDS) are hospitalized for cardiorespiratory monitoring however, monitoring is costly and generates large quantities of averaged data that serve as poor predictors of infant risk. In this study we used a traditional autocorrelation function (ACF) testing its suitability as a tool to detect subtle alterations in respiratory patterning in vivo. We applied the ACF to chest wall motion tracings obtained from rat pups in the period corresponding to the mid-to-end of the third trimester of human pregnancy. Pups were drawn from two groups: nicotine-exposed and saline-exposed at each age (i.e., P7, P8, P9, and P10). Respiratory-related motions of the chest wall were recorded in room air and in response to an arousal stimulus (FIO2 14%). The autocorrelation function was used to determine measures of breathing rate and respiratory patterning. Unlike alternative tools such as Poincare plots that depict an averaged difference in a measure breath to breath, the ACF when applied to a digitized chest wall trace yields an instantaneous sample of data points that can be used to compare (data) points at the same time in the next breath or in any subsequent number of breaths. The moment-to-moment evaluation of chest wall motion detected subtle differences in respiratory pattern in rat pups exposed to nicotine in utero and aged matched saline-exposed peers. The ACF can be applied online as well as to existing data sets and requires comparatively short sampling windows (∼2 min). As shown here, the ACF could be used to identify factors that precipitate or minimize instability and thus, offers a quantitative measure of risk in vulnerable populations.


Asunto(s)
Envejecimiento/efectos de los fármacos , Barbitúricos/farmacología , Nicotina/farmacología , Agonistas Nicotínicos/farmacología , Respiración/efectos de los fármacos , Factores de Edad , Animales , Animales Recién Nacidos , Oscilación de la Pared Torácica/métodos , Femenino , Masculino , Ratas , Ratas Sprague-Dawley
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