RESUMEN
With a view to producing peptides capable of inducing a protective immune response against Schistosoma mansoni and Fasciola hepatica, the sequence and structure of the protective antigens Sm14 and Fh15 were analyzed. Their C-termini showed a high level of sequence conservation which, together with models for their three-dimensional structures, aided in peptide selection. Vaccination trials in Swiss mice challenged with S. mansoni cercaria or F. hepatica metacercaria showed that peptides which included the sequences VTVGDVTA or EKNSESKLTQ were capable of inducing levels of protection equivalent to the recombinant form of Sm14. These peptides may represent an alternative to r-Sm14 for the development of a bivalent anti-helminth vaccine.
Asunto(s)
Fascioliasis/inmunología , Fascioliasis/prevención & control , Esquistosomiasis mansoni/inmunología , Esquistosomiasis mansoni/prevención & control , Vacunas Combinadas/inmunología , Vacunas de Subunidad/inmunología , Secuencia de Aminoácidos , Animales , Anticuerpos Antihelmínticos/análisis , Anticuerpos Antihelmínticos/biosíntesis , Diseño de Fármacos , Fasciola hepatica/inmunología , Femenino , Inmunización , Ratones , Datos de Secuencia Molecular , Péptidos/síntesis química , Péptidos/inmunología , Conformación Proteica , Schistosoma mansoni/inmunologíaRESUMEN
UNLABELLED: A web-based software suite, SABIA (System for Automated Bacterial Integrated Annotation), is described that provides a comprehensive computational support for the assembly and annotation of whole bacterial genomes from the data derived from sequencing projects. AVAILABILITY: Both SABIA and supplementary materials are available at http://www.sabia.lncc.br