Asunto(s)
Anticuerpos Antibacterianos/análisis , Orientia tsutsugamushi/inmunología , Tifus por Ácaros/inmunología , Complemento C3/análisis , Humanos , Inmunoglobulina A/análisis , Inmunoglobulina G/análisis , Inmunoglobulina M/análisis , Recuento de Leucocitos , Activación de Linfocitos , Masculino , Linfocitos T/inmunología , Taiwán , Factores de TiempoRESUMEN
The Unicam Spectrophotometer, the IL 282 CO-Oximeter, and the Hemoscan were used to measure the in vitro P50 using 1, 20--45, and 50% hematocrit values of red blood cells suspended in autologous plasma or phosphate buffered saline at pH of 6.7--7.7. The relation between pH and P50 was a linear correlation for washed red blood cells by all three methods whereas the relation between pH and P50 was a log correlation for red blood cells in plasma. Multiple units of red blood cells with low 2,3 diphosphoglycerate (2,3 DPG) and increased affinity for oxygen, with normal 2,3 DPG and normal affinity for oxygen, and above normal 2,3 DPG and decreased affinity for oxygen, were frozen in 10 ml aliquots with 40% w/v glycerol and maintained at -80 degrees C. One tube of each level of 2.3 DPG was thawed, washed, and suspended in buffer daily to yield P50 values of 17.5 +/- 0.67, 29.7 +/- 0.92, or 41.2 +/- 0.72 torr at pH 7.2 in order to have a control of both instrumentation and personnel.
Asunto(s)
Eritrocitos , Oxígeno/sangre , Ácidos Difosfoglicéricos/sangre , Congelación , Hematócrito , Humanos , Concentración de Iones de Hidrógeno , Oxihemoglobinas/metabolismo , Presión ParcialRESUMEN
The intracellular distribution of radioactivity was studied in normal and sickle erythrocytes labeled with sodium 51Cr chromate. Both types of cells had a higher fraction of 51Cr bound to hemoglobin when labeled in the presence of ACD at a pH of 7.09 than when labeled in the presence of CPD at a pH of 5.96. Citrate at a pH of 5.96 or less entered the red blood cells and decreased the 51Cr binding hemoglobin. Binding of 51Cr to hemoglobin within the red cells was also reduced when the ATP and DPG levels in the red blood cells were elevated. Studies with hemoglobin solution showed that 51Cr binding to hemoglobin was influenced by 2,3-DPG, ATP and citrate.
Asunto(s)
Compuestos de Cromo , Radioisótopos de Cromo , Ácido Cítrico , Cloruros/metabolismo , Cromatografía en Gel , Cromatografía en Capa Delgada , Cromo/metabolismo , Citratos/farmacología , Envejecimiento Eritrocítico , Glucosa/análogos & derivados , Glucosa/farmacología , Glucofosfatos/farmacología , Hemólisis , Humanos , Rasgo Drepanocítico/sangreRESUMEN
Four anticoagulant solutions were added to baboon red blood cells prior to labeling with 51Cr to determine how each would influence the distribution of 51Cr within the red blood cells, the loss of 51Cr from the red blood cells after transfusion, and the calculated red cell survival value. The 51Cr label was detected in the hemoglobin and in the low molecular weight compounds within the red blood cells. The elution of 51Cr from labeled baboon red blood cells following transfusion could not be explained by the distribution of 51Cr between hemoglobin and low molecular weight compounds within the red blood cells.