[The comparison of tests for qualitative evaluation of Ureaplasma parvum, Mycoplasma hominis: "Mycoplasma duo", "Ureaplasma microtest", "Mycoplasma microtest" and "AmpliSens-Florocenosis-mycoplasma-FL"].

The genital mycoplasma is an opportunistic bacteria and its detection is to be implemented in qualitative format. The study was organized to compare reagents kits "Mycoplasma Duo", "Ureaplasma Microtest", "Mycoplasma microtest" and "AmpliSens-Florocenosis-Mycoplasma-FL". The study resulted in high indicators of diagnostic sensitivity and diagnostic specificity for all kits. At that, the lowest indicators were registered under application of "Mycoplasma Duo" kit. The study reveled correlation of qualitative values detected by using cultural analysis and polymerase chain reaction. The reproducibility of qualitative values of cultural method occurred significantly lower in comparison with "AmpliSens-Florocenosis-Mycoplasma-FL " kit.

Glucocorticoid regulation of phospholipid turnover and protein kinase C activity in mouse hepatoma 22 cells.

Glucocorticoids induce growth inhibition in certain sensitive hepatoma cells. To investigate how glucocorticoids interact with growth-factor-dependent pathways, we studied the effects of dexamethasone (Dex) on the DNA synthesis, protein kinase C (PKC) activity and phospholipid turnover in mouse hepatoma 22 cells. Dex was found to reduce DNA synthesis in slowly growing hepatoma cells, whereas exponentially growing cells were Dex-insensitive. Direct measurements of PKC activity in the hormone-sensitive hepatoma 22 cells showed a rapid inhibition (within 30 min) when treated with Dex. Dex addition to hormone-sensitive but not to hormone-insensitive hepatoma 22 cells for 30 min caused a significant decrease of 32P-incorporation into the major cellular phospholipids: phosphatidylcholine, phosphatidylglycerol and phosphoinositides. At the same time, the analysis of the correlation between changes in PKC activity and phospholipid turnover showed that synthesis of phosphatidylcholine and phosphatidylglycerol was under positive control of PKC activity. The data suggest that suppression of phospholipid turnover in hormone-sensitive hepatoma 22 cells is one of the early events caused by glucocorticoids, whereas the decrease of PKC activity induced by the hormone is mediated, probably, via changes in phospholipid metabolism.

[The evaluation of the diagnostic value of PCR test systems according to the results of state trials].

The method for the diagnostic value evaluation of preparations, based on the calculation of the accuracy of test results and taking into account the spread of the diagnosed disease (the comparison of the PCR test systems for the diagnostics of hepatitis B and ureaplasmosis) is proposed. As shown in this work, evaluations obtained with the use of this method coincide with those obtained on the basis of prognostic value, but are more convenient in use and provide additional information.

[Cloning and analysis of genetic determinants determining the production of microcin B2 and B27]. / Klonirovanie i analiz geneticheskikh determinantov, opredeliaiushchikh produktsiiu mikrotsinov B2 i B27.

Cloning of plasmid genes for the synthesis of two peptide broad-spectrum antibiotics-microcins B2 and B27- and for host cell immunity to their action was performed. Recombinant plasmids containing these genes were designated pBE108 and pVB27, respectively. Deletional derivatives of plasmid pBE108 and mutant plasmids were obtained via transposon Tn5 insertions, which did not determine production of microcin B2 and immunity to it. Phenotypic study and physical mapping of these plasmids demonstrated that a 4.2-kb DNA fragment is responsible for B2 microcin production; immunity is provided by a 1.4-kb DNA fragment. A 5-kb DNA fragment is necessary for microcin B27 synthesis and expression of immunity to its action. Homology between these fragments and with plasmid DNA for the synthesis of microcin B17 and immunity to it was found. Homology between plasmid genes determining synthesis of type B and C microcins and host cell immunity to them was not observed. The production of B27 microcin is controlled by the product of the ompR gene; B2 microcin synthesis does not depend on this product. The mutations recA and lexA increase the susceptibility of Escherichia coli cells to the action of microcins B2 and B27 but not microcin C51.

[Identification and expression of plasmid genes participating in the synthesis of microcin C51]. / Identifikatsiia i ékspressiia plazmidnykh genov, uchastvuiushchikh v sinteze mikrotsina C51.

A structural gene of heptapeptide, which is a component of the microcin C51 molecule, was identified by hybridization of plasmid DNA fragments and a mixture of synthesized oligonucleotides with the sequence corresponding to that of amino acids in the peptide. Sequence analysis of the structural gene of microcin peptide and its promoter region was performed. The data obtained indicate that the peptide of microcin C51 is synthesized on ribosomes. Four polypeptides of 67, 39, 16, and 14 kDa were identified using the system of minicells. These polypeptides are specified by a DNA fragment responsible for microcin synthesis and immunity in a producer cell. Apparently, three of these polypeptides with molecular masses of 67, 39, and 16 kDa are responsible for microcin production and immunity. The 67 kDa polypeptide is involved in the expression of immunity to microcin and, probably, in microcin production.

[Comparative evaluation of diagnostic preparations]. / Sravnitel'naia otsenka diagnosticheskikh preparatov.

The diagnostic value of preparations is commonly characterized by sensitivity and specificity. But not all these characteristics make it possible to decide unequivocally which of the preparations to be compared is superior to the other one with respect to its diagnostic value. It is proposed that in the choice of a diagnostic preparation its capacity to provide data for exact diagnosis should be considered, i.e. the additional characteristic indicating the spread of the disease under study. As an example, the comparison of the diagnostic value of conventional methods and the polymerase chain reaction in the diagnostics of helicobacteriosis is presented. The described method for the evaluation of the diagnostic value of the preparation is well-grounded, simple and obvious.

[Systemic inflammatory response syndrome and sepsis in maxillofacial surgery clinic]. / Sindrom sistemnogo vospalitel'nogo otveta i sepsis v klinike cheliustno-litsevoi khirurgii.

A total of 106 patients with surgical infection in maxillofacial region were studied. Clinical and laboratory diagnostic data on surgical infection and sepsis in maxillofacial surgery are presented. Integral indices of blood circulation, respiration, brain and liver metabolism, regarding severity of surgical infection were determined. Pathogenetically substantiated therapy of sepsis is proposed.

[The status of research in dentistry]. / Sostoianie nauchnykh issledovanii v stomatologii.

Discusses the problems of market relationships in Russian dentistry and insurance medicine: various forms of intellectual property, the market of equipment and materials for dentistry, such forms as lease contract or working place lease. The authors emphasize the contribution of the intellectual potential to the development of novel equipment and materials and clinical methods.

[Use of a nasolabial flap for correcting defects of the hard palate]. / Ispol'zovanie noso-gubnogo loskuta dlia zameshcheniia defektov tverdogo neba.

A method of surgical elimination of postoperative great defects of the hard palate is described. The method is based on using a nasolabial flap. The method was successfully used in 15 patients with this pathology.

[Stress on development of glossalgia and validation of treatment]. / Rol' stressa v vozniknovenii glossalgii i obosnovanie lecheniia.

Study of psychoemotional and neurological status of 58 patients with glossalgia showed that stress plays the leading role in development of this abnormality. Neurophysiological analysis of electroencephalograms of patients with glossalgia revealed pronounced generalized restructuring of rhythmic activity of the brain. Ultrasonic dopplerography of the tongue vessels and laser Doppler flowmetry of capillary bloodflow in the tongue confirmed the presence of short-term ischemia in the tongue at the moment of painful attack. Use of modern anxiolytics and antidepressants in combination with ozone therapy in combined therapy of patients with glossalgia is validated.

[Hydroxyapatite as a substrate for osteoplasty: the theoretical and practical aspects of the problem]. / Gidroksiapatit kak substrat dlia kostnoi plastiki: teoreticheskie i prakticheskie aspekty problemy.

Discusses the state of the art in the use of hydroxyapatite-based materials for osteoplasty. Analysis of published data and their own findings brought the authors to a conclusion that various types of hydroxyapatite cause different biological effects. Use of these materials requires a strictly differentiated approach, depending on the tasks to solve.

[The surgical treatment of patients with extensive defects of the soft palate]. / Khirurgicheskoe lechenie bol'nykh s obshirnymi defektami miagkogo neba.

A modified technique of velopharyngoplasty is described, involving preliminary epithelialization of the total wound surface of the musculomucous flap and the newly formed wound defect on the posterior wall of the pharynx by submerged skin transplantation. The results of treatment of 12 patients with various types of postoperative extensive defects of the soft palate demonstrate a high efficacy of the developed technique.

[The classification of diseases of the temporomandibular joint]. / Klassifikatsiia zabolevanii visochno-nizhnecheliustnogo sustava.

There were 2040 patients with temporomandibular joint (TMJ) diseases in the investigation including clinical, physiological, X-ray methods of research, contrast arthrotomography, computer arthrotomography and magnetic resonance tomography. The changes of structure in different lesions were estimated. The result of received data was elaboration of classification of TMJ internal derangements with estimation of clinical forms in according with structural changes and characteristics of syndromes.

[Results and perspectives of development in dental implantation in Russia]. / Rezul'taty i perspektivy issledovaniia problem dental'noi implantologii v Rossii.

The basic trends of scientific research carried out at the implantation department of CRID (Central Research Institute of Dentistry), referred to the fundamental problems in medicine and biology including a variety of not only experiments and clinical study but also a complex of technical aspects. Our long-term research and valuable clinical material collected resulted in confirming the necessity and importance of complex approaches developed by the Institute in dental implantation.

[Medical rehabilitation of patients with atrophic jaws]. / Meditsinskaia reabilitatsiia bol'nykh so znachitel'noi atrofiei cheliustei.

The results of this study indicate that subperiosteal implants extend the potentialities of treatment for patients with significant maxillary bone atrophy fitted with permanent dentures.

[Gun-shot wounds of the face under conditions of local and boundary conflicts]. / Ognestrel'nye raneniia litsa v usloviiakh lokal'nykh i prigranichnykh konfliktov.

Analysis of the incidence, types, and severity of maxillofacial wounds inflicted during local and boundary conflicts showed increased incidence of the most severe wounds with injuries to both jaws. The efficiency of medical care rendered to victims with facial wounds is determined by the level of specialized training of nurses and physician's assistants.

[X-ray assessment of the size of the nasopharynx]. / Rentgenologicheskaia otsenka razmerov nosoglotki.

A new method for x-ray diagnosis of nasopharyngeal size is based on the use of long-distance roentgenography in the lateral projection and of skeletal markers to estimate the cephalometric parameters. The authors draw attention to the usefulness of measuring the size of bone base, soft-tissue structures, and nasopharyngeal air column when assessing the function of the nasopharynx and throat.

[An x-ray method for determining the position of the tongue in the oral cavity]. / Rentgenologicheskii sposob opredeleniia polozheniia iazyka v polosti rta.

The suggested modified technique for assessing the tongue position in the oral cavity is based on teleroentgenography in the lateral projection and estimation of cephalometric parameters. Theoretical rationale is given for the use of skeletal check points in the mandibular area and cervical portion of the spine and of the mandibular and spinal reference lines. Indications for the use of this method are defined and its advantages described.

[Anesthesia in cranio-facial surgery]. / Anesteziia v kraniofatsial'noi khirurgii.

Reconstructive operations on the facial skeleton and on the skull have been widely introduced in surgery. Increment of indications and augmentation of traumaticity from surgical interventions pose to anesthesiologist a set of such problems, as tracheal intubation, antinociceptive protection, prevention of hepatocerebral encephalopathy. The peculiarities of cerebral and hepatic circulation and metabolism as well as approaches in anesthesia and craniofacial surgery are discussed.

[The use of titanium plates and endoprostheses for the mandibular condyles in reconstructive surgery of the mandible]. / Ispol'zovanie titanovykh plastin i éndoprotezov myshchelkovykh otrostkov v rekonstruktivnoi khirurgii nizhnei cheliusti.

Potentialities of titanium plates and endoprostheses of the condyle processes in replacement of the postoperative defects of mandibular bone tissue are discussed. A recently developed Russian kit of titanium plates and instruments and specificities of plasty to repair bone defects using this kit are described. The results of surgical repair in 5 patients subjected to resection of the mandible with exarticulation for tumors or osteomyelitis aftereffects were good: the oval of the face and mandibular function were repaired.