Determination of antifungal efficacy and phytotoxicity of a unique silica coated porous zinc oxide nanocomposite medium for slow-release agrochemicals.

AIMS: In this study, the antifungal efficacy and phytotoxicity of silica coated porous zinc oxide nanoparticle (SZNP) were analyzed as this nanocomposite was observed to be a suitable platform for slow release fungicides and has the promise to bring down the dosage of other agrochemicals as well. METHODS AND RESULTS: Loading and release kinetics of tricyclazole, a potent fungicide, were analyzed by measuring surface area (SBET) using Brunauer-Emmett-Teller (BET) isotherm and liquid chromatography tandem mass spectrometry (LC-MS/MS), respectively. The antifungal efficacy of ZnO nanoparticle (ZNP) and SZNP was investigated on two phytopathogenic fungi (Alternaria solani and Aspergillus niger). The morphological changes to the fungal structure due to ZNP and SZNP treatment were studied by field emission-scanning electron microscopy. Nanoparticle mediated elevation of reactive oxygen species (ROS) in fungal samples was detected by analyzing the levels of superoxide dismutase, catalase, thiol content, lipid peroxidation, and by 2,7-dichlorofluorescin diacetate assay. The phytotoxicity of these two nanostructures was assessed in rice plants by measuring primary plant growth parameters. Further, the translocation of the nanocomposite in the same plant model system was examined by checking the presence of fluorescein isothiocyanate tagged SZNP within the plant tissue. CONCLUSIONS: ZNP had superior antifungal efficacy than SZNP and caused the generation of more ROS in the fungal samples. Even then, SZNP was preferred as an agrochemical delivery vehicle because, unlike ZNP alone, it was not toxic to plant system. Moreover, as silica in nanoform is entomotoxic in nature and nano ZnO has antifungal property, both the cargo (agrochemical) and the carrier system (silica coated porous nano zinc oxide) will have a synergistic effect in crop protection.

Cyanometabolites: molecules with immense antiviral potential.

Cyanometabolites are active compounds derived from cyanobacteria that include small low molecular weight peptides, oligosaccharides, lectins, phenols, fatty acids, and alkaloids. Some of these compounds may pose a threat to human and environment. However, majority of them are known to have various health benefits with antiviral properties against pathogenic viruses including Human immunodeficiency virus (HIV), Ebola virus (EBOV), Herpes simplex virus (HSV), Influenza A virus (IAV) etc. Cyanometabolites classified as lectins include scytovirin (SVN), Oscillatoria agardhii agglutinin (OAAH), cyanovirin-N (CV-N), Microcystis viridis lectin (MVL), and microvirin (MVN) also possess a potent antiviral activity against viral diseases with unique properties to recognize different viral epitopes. Studies showed that a small linear peptide, microginin FR1, isolated from a water bloom of Microcystis species, inhibits angiotensin-converting enzyme (ACE), making it useful for the treatment of coronavirus disease 2019 (COVID-19). Our review provides an overview of the antiviral properties of cyanobacteria from the late 90s till now and emphasizes the significance of their metabolites in combating viral diseases, particularly severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which has received limited attention in previous publications. The enormous medicinal potential of cyanobacteria is also emphasized in this review, which justifies their use as a dietary supplement to fend off pandemics in future.

Emergence and impact of theranostic-nanoformulation of triple therapeutics for combination cancer therapy.

Cancer remains a major global health threat necessitating the multipronged approaches for its prevention and management. Traditional approaches in the form of chemotherapy, surgery, and radiotherapy are often encountered with poor patient outcomes evidenced by high mortality and morbidity, compelling the need for precision medicine for cancer patients to enable personalized and targeted cancer treatment. There has been an emergence of smart multimodal theranostic nanoformulation for triple combination cancer therapy in the last few years, which dramatically enhances the overall safety of the nanoformulation for in vivo and potential clinical applications with minimal toxicity. However, it is imperative to gain insight into the limitations of this system in terms of clinical translation, cost-effectiveness, accessibility, and multidisciplinary collaboration. This review paper aims to highlight and compare the impact of the recent theranostic nanoformulations of triple therapeutics in a single nanocarrier for effective management of cancer and provide a new dimension for diagnostic and treatment simultaneously.

Structural and Surface Properties of pH-Varied Fe2O3 Nanoparticles: Correlation with Antibacterial Properties.

Hematite (Fe2O3) nanoparticles were synthesized using a hydrothermal synthesis route under different pH conditions (pH ∼8,10,11.5) (i.e., different ratios of H+/OH- ions). The sample synthesized at pH 10 had better motility toward the bacterial surface due to having an overall positive charge (ξ-potential = +11.10), leading to a minimal hydrodynamic size (Dτ = 186.6). The results are discussed in light of the relative ratio of H+/OH- that may affect bond formation by influencing the electronic clouds of the participating ions that can modify the structure. This, in turn, modifies crystallinity, strain, disorder, surface termination, and thereby, the surface charge, which has been correlated to the antibacterial properties of the nanoparticles due to the interaction between the respective opposite charges on the nanoparticle surface and bacterial cell wall. The structural modifications were correlated to all of these parameters in this work.

Multifunctional Iridium(III)-Platinum(IV) Conjugates as Potent Anticancer Theranostic Agents.

In this article, we report IriPlatins 1-3, a new class of heterobimetallic Ir(III)-Pt(IV) conjugates as multifunctional potent anticancer theranostic agents. In the designed construction, the octahedral Pt(IV) prodrug is tethered to the cancer cell targeting biotin ligand through one of the axial sites and the other axial site of Pt(IV) center is attached to multifunctional Ir(III) complexes that possess organelle-targeting capabilities with excellent anticancer and imaging properties. The conjugates preferentially accumulate within the mitochondria of cancer cells, and subsequently, Pt(IV) is reduced to Pt(II) species that concomitantly releases both the Ir(III) complex and biotin from its axial sites. The IriPlatin conjugates demonstrate potent anticancer activity in various 2D monolayer cancer cells, including the cisplatin-resistant cells in the nanomolar concentrations and 3D multicellular tumor spheroids. The mechanistic investigation of conjugates suggests that the loss of MMP, generation of ROS, and caspase-3-mediated apoptosis are responsible for cell death.

Studying the structural organization of non-membranous protein hemoglobin in a lipid environment after reconstitution.

In our current work we have developed a supported 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayer with embedded hemoglobin, reconstituted via detergent-mediated method. Microscopic studies revealed that the hemoglobin molecules could be visualized without any labelling agents. The reconstituted proteins assemble themselves as supramolecular structures to adapt to lipid bilayer environment. The nonionic detergent, n-octyl-ß-d-glucoside (NOG) used for insertion of hemoglobin played an important role in formation of these structures. When concentrations of lipid, protein and detergent were raised by four folds, we observed phase separation by protein molecules within bilayer via protein-protein assembly. This phase separation process exhibited extremely slow kinetics to form large stable domains with correlation times in the order of minutes. Confocal Z-scanning images showed that these supramolecular structures generated membrane deformities. UV-Vis, Fluorescence and Circular Dichroism (CD) measurement indicated minor structural change to expose the hydrophobic regions of the protein to adjust the hydrophobic stress of the lipid environment whilst Small Angle Neutron Scattering (SANS) results indicated that the hemoglobin molecules retained their overall tetrameric form in the system. In conclusion, we state that this investigation allowed us to closely inspect some rare but noteworthy phenomena like the formation of supramolecular structures, large domain formation and membrane deformation etc.

Formation of Self-Assembled Nanowires from Copper Nanoparticles Synthesized by the Electro-Explosion of Wires Technique-Study of the Time-Dependent Structural and Functional Evolution.

We report here the formation of Cu nanowires (CuNWs) from Cu nanoparticles (CuNPs) by a self-assembly process. The CuNPs were synthesized by the electro-explosion of wire (EEW) technique that included nonequilibrium processes for the synthesis. Structural evolution in terms of aggregation or nanowire formation in the samples was observed when the CuNPs were kept for a month after synthesis in a glass vial without the application of any external driving force. The emergence of tangled CuNWs was noticed at the bottom of the vials only when no agitation or aeration was allowed. The nanowires were characterized using transmission electron microscopy (TEM) and X-ray diffraction (XRD). Thermal oxidation of the nanowire samples implied that they could convert into rod-shaped structures. Loss of functionality was also observed in the hemoglobin precipitation study conducted to compare the activity of freshly prepared CuNPs and CuNWs. From the above observations, we conclude that the CuNP, after synthesis, possesses a huge amount of energy, and attainment of equilibrium occurs through either aggregation (clustering) or ordered self-assembly, depending on the conditions applied.

A Comprehensive Review on Anti-Inflammatory Response of Flavonoids in Experimentally-Induced Epileptic Seizures.

Flavonoids, a group of natural compounds with phenolic structure, are becoming popular as alternative medicines obtained from plants. These compounds are reported to have various pharmacological properties, including attenuation of inflammatory responses in multiple health issues. Epilepsy is a disorder of the central nervous system implicated with the activation of the inflammatory cascade in the brain. The aim of the present study was to summarize the role of various neuroinflammatory mediators in the onset and progression of epilepsy, and, thereafter, to discuss the flavonoids and their classes, including their biological properties. Further, we highlighted the modulation of anti-inflammatory responses achieved by these substances in different forms of epilepsy, as evident from preclinical studies executed on multiple epilepsy models. Overall, the review summarizes the available evidence of the anti-inflammatory potential of various flavonoids in epilepsy.

Sugar osmolyte inhibits and attenuates the fibrillogenesis in RNase A: An in vitro and in silico characterizations.

Mechanisms of protein aggregation are of immense interest in therapeutic biology and neurodegenerative medicine. Biochemical processes within the living cell occur in a highly crowded environment. The phenomenon of macromolecular crowding affects the diffusional and conformational dynamics of proteins and modulates their folding. Macromolecular crowding is reported to cause protein aggregation in some cases, so it is a cause of concern as it leads to a plethora of neurodegenerative disorders and systemic amyloidosis. To divulge the mechanism of aggregation, it is imperative to study aggregation in well-characterized model proteins in the presence of macromolecular crowder. One such protein is ribonuclease A (RNase A), which deciphers neurotoxic function in humans; therefore we decided to explore the amyloid fibrillogenesis of this thermodynamically stable protein. To elucidate the impact of crowder, dextran-70 and its monomer glucose on the aggregation profile of RNase-A various techniques such as Absorbance, Fluorescence, Fourier Transforms Infrared, Dynamic Light Scattering and circular Dichroism spectroscopies along with imaging techniques like Atomic Force Microscopy and Transmission Electron Microscopy were employed. Thermal aggregation and fibrillation were further promoted by dextran-70 while glucose counteracted the effect of the crowding agent in a concentration-dependent manner. This study shows that glucose provides stability to the protein and prevents fibrillation. Intending to combat aggregation, which is the hallmark of numerous late-onset neurological disorders and systemic amyloidosis, this investigation unveils that naturally occurring osmolytes or other co-solutes can be further exploited in novel drug design strategies.

Green synthesis of silver nanoparticles using Ocimum sanctum Linn. and its antibacterial activity against multidrug resistant Acinetobacter baumannii.

The biosynthesis of nanoparticles using the green route is an effective strategy in nanotechnology that provides a cost-effective and environmentally friendly alternative to physical and chemical methods. This study aims to prepare an aqueous extract of Ocimum sanctum (O. sanctum)-based silver nanoparticles (AgNPs) through the green route and test their antibacterial activity. The biosynthesized silver nanoparticles were characterised by colour change, UV spectrometric analysis, FTIR, and particle shape and size morphology by SEM and TEM images. The nanoparticles are almost spherical to oval or rod-shaped with smooth surfaces and have a mean particle size in the range of 55 nm with a zeta potential of -2.7 mV. The antibacterial activities of AgNPs evaluated against clinically isolated multidrug-resistant Acinetobacter baumannii (A. baumannii) showed that the AgNPs from O. sanctum are effective in inhibiting A. baumannii growth with a zone of inhibition of 15 mm in the agar well diffusion method and MIC and MBC of 32 µg/mL and 64 µg/mL, respectively. The SEM images of A. baumannii treated with AgNPs revealed damage and rupture in bacterial cells. The time-killing assay by spectrophotometry revealed the time- and dose-dependent killing action of AgNPs against A. baumannii, and the assay at various concentrations and time intervals indicated a statistically significant result in comparison with the positive control colistin at 2 µg/mL (P < 0.05). The cytotoxicity test using the MTT assay protocol showed that prepared nanoparticles of O. sanctum are less toxic against human cell A549. This study opens up a ray of hope to explore the further research in this area and to improve the antimicrobial activities against multidrug resistant bacteria.

Antibiotic-Loaded Smart Platelet: A Highly Effective Invisible Mode of Killing Both Antibiotic-Sensitive and -Resistant Bacteria.

Microbial pathogenesis is considered one of the most critical health challenges worldwide. Although several antibiotics have been procured and used, the microbes often manage to escape and become resistant to antibiotics. Thus, the discovery of new antibiotics and designing smart approaches toward their delivery are of great importance. In many cases, the delivery agents using foreign chemicals like lipids or polymers induce immunogenic responses of varying degrees and are limited to a shorter circulatory time and burst release. In the current work, we have designed a novel antibiotic delivery system where the antibiotic is encapsulated into a blood component-platelet. Platelets have been previously reported as efficient drug delivery vehicles for targeting cancer cells. On the other hand, during platelet-bacterial interaction, platelets can act as covercytes. Keeping this in mind, smart antibiotic-loaded platelets have been used for killing bacterial cells. The loading of the antibiotic was done using its typical nature of engulfing surrounding small molecules. The water-soluble antibiotics were loaded directly into the platelet, whereas the hydrophobic antibiotics were preloaded in polycaprolactone (FDA-approved polymer)-based nanovesicles to make them solubilized prior to loading inside the platelets. The antibiotic-loaded platelets (containing hydrophilic antibiotics or hydrophobic antibiotic -encapsulated polymer nanoparticles) were found to be stable when studied through platelet aggregometry. The carrier showed bactericidal effects at a significantly lower concentration at which the free antibiotic has negligible efficacy. This could be attributed to the molecular confinement of the antibiotics inside the platelets, therefore causing localization of the drug and leading to efficient activity against bacteria. Interestingly, the smart antibiotic-loaded platelets were capable of killing the resistant strains too at the same lower concentration regime. Therefore, the antibiotic-loaded platelet could emerge as a potential strategy for efficient delivery of antibiotics with a significant reduction of the dose required to achieve the intended antibacterial efficacy. Moreover, this antibiotic delivery method can be very useful to minimize immunogenic responses due to antibiotic administration and to avoid the development of drug resistance due to the invisible mode of delivery.

Natural Occurrence of Carbon Dots during In Vitro Nonenzymatic Glycosylation of Hemoglobin A0.

Carbon dots, the nanostructures of carbon, have excellent optical and chemical properties and find a range of applications in various fields of biology and medicine. In the current study, carbon dots are synthesized using in vitro nonenzymatic glycosylation at 37 °C, which is the conventional method for the synthesis of Advanced Glycosylation End products. While comparing the physicochemical properties using a series of physical and chemical analyses including light absorption, fluorescence, photoluminescence, chemical composition, functional group analysis, and in vitro imaging, striking similarities are found among Carbon dots and Advanced Glycosylation End products. Based on the evident resemblance between the two, we propose either the presence of a common structural backbone or the coexistence of the two individual chemical entities. Thus, the formation of carbon dots at physiological temperatures raises health concerns as nonenzymatic glycosylation is a physiological process in humans and the rate of which is elevated during diabetes. The Advanced Glycosylation End products are known to have a detrimental effect in diabetic patients, and the chemical similarity between the two questions the widely studied biocompatibility of carbon dots.

Recent Advances in Cancer Vaccines: Challenges, Achievements, and Futuristic Prospects.

Cancer is a chronic disease, and it can be lethal due to limited therapeutic options. The conventional treatment options for cancer have numerous challenges, such as a low blood circulation time as well as poor solubility of anticancer drugs. Therapeutic cancer vaccines emerged to try to improve anticancer drugs' efficiency and to deliver them to the target site. Cancer vaccines are considered a viable therapeutic technique for most solid tumors. Vaccines boost antitumor immunity by delivering tumor antigens, nucleic acids, entire cells, and peptides. Cancer vaccines are designed to induce long-term antitumor memory, causing tumor regression, eradicate minimal residual illness, and prevent non-specific or unpleasant effects. These vaccines can assist in the elimination of cancer cells from various organs or organ systems in the body, with minimal risk of tumor recurrence or metastasis. Vaccines and antigens for anticancer therapy are discussed in this review, including current vaccine adjuvants and mechanisms of action for various types of vaccines, such as DNA- or mRNA-based cancer vaccines. Potential applications of these vaccines focusing on their clinical use for better therapeutic efficacy are also discussed along with the latest research available in this field.

Drug Delivery Strategies and Biomedical Significance of Hydrogels: Translational Considerations.

Hydrogels are a promising and attractive option as polymeric gel networks, which have immensely fascinated researchers across the globe because of their outstanding characteristics such as elevated swellability, the permeability of oxygen at a high rate, good biocompatibility, easy loading, and drug release. Hydrogels have been extensively used for several purposes in the biomedical sector using versatile polymers of synthetic and natural origin. This review focuses on functional polymeric materials for the fabrication of hydrogels, evaluation of different parameters of biocompatibility and stability, and their application as carriers for drugs delivery, tissue engineering and other therapeutic purposes. The outcome of various studies on the use of hydrogels in different segments and how they have been appropriately altered in numerous ways to attain the desired targeted delivery of therapeutic agents is summarized. Patents and clinical trials conducted on hydrogel-based products, along with scale-up translation, are also mentioned in detail. Finally, the potential of the hydrogel in the biomedical sector is discussed, along with its further possibilities for improvement for the development of sophisticated smart hydrogels with pivotal biomedical functions.

Sunlight mediated enhanced photocatalytic activity of TiO2 nanoparticles functionalized CuO-Cu2O nanorods for removal of methylene blue and oxytetracycline hydrochloride.

Metal free heterojunctions have shown promising applicability as potential photocatalyst materials. Like the commonly explored metal-non metal heterojunctions, semiconductor-semiconductor junctions are also capable of facilitating charge separation and improved lifetimes, leading to augmented surface reaction efficacy. However, unlike the metal carrying heterojunctions, they are much economical and easier to fabricate and tune. Through this study, we present a facile one step hydrothermal route to synthesize CuO-Cu2O nanorods/TiO2 nanoparticles heterostructures (CTHS) with their potential application as a low cost photocatalytic alternative. The average size of the synthesized heterojunction components, as estimated from transmission electron microscopy (TEM) evaluation was 13 and 5 nm respectively for the nanorod length and width, while the functionalizing TiO2 nanoparticles were averaged around 10 nm. Heterojunction formation was confirmed using Raman spectroscopy, X-ray diffraction, high resolution TEM, and elemental mapping. X-ray photoelectron spectroscopy data marked with presence of Cu+ and Cu+2 state of CuO in CuXO-TiO2 also supported junction formation. Optical characteristics of the heterojunction were studied using UV-vis diffuse reflectance spectroscopy and photoluminescence spectroscopy. Compared to TiO2 nanoparticles, CTHS exhibited superior sunlight-induced photodegradation activity. CuXO/TiO2 heterojunction could also remediate toxic waste water containing model antibiotic residue (Oxytetracycline hydrochloride, 0.4 mg/mL) and organic pollutant (methylene blue, 10 µM) in 20 and 60 min respectively. Ultra-fast degradation using a nonmetal heterojunction nanohybrid, like ours, finds negligible mention in literature. Improved visible light absorption and reduction in recombination rate for CuXO-TiO2 nanohybrids were ascribed as major contributing factors towards their enhanced photocatalytic potential. The charge separation mechanism for nanohybrids has been studied and elaborated in detail.

Current Insights and Advancements in Head and Neck Cancer: Emerging Biomarkers and Therapeutics with Cues from Single Cell and 3D Model Omics Profiling.

Head and neck cancer (HNC) is among the ten leading malignancies worldwide, with India solely contributing one-third of global oral cancer cases. The current focus of all cutting-edge strategies against this global malignancy are directed towards the heterogeneous tumor microenvironment that obstructs most treatment blueprints. Subsequent to the portrayal of established information, the review details the application of single cell technology, organoids and spheroid technology in relevance to head and neck cancer and the tumor microenvironment acknowledging the resistance pattern of the heterogeneous cell population in HNC. Bioinformatic tools are used for study of differentially expressed genes and further omics data analysis. However, these tools have several challenges and limitations when analyzing single-cell gene expression data that are discussed briefly. The review further examines the omics of HNC, through comprehensive analyses of genomics, transcriptomics, proteomics, metabolomics, and epigenomics profiles. Patterns of alterations vary between patients, thus heterogeneity and molecular alterations between patients have driven the clinical significance of molecular targeted therapies. The analyses of potential molecular targets in HNC are discussed with connotation to the alteration of key pathways in HNC followed by a comprehensive study of protein kinases as novel drug targets including its ATPase and additional binding pockets, non-catalytic domains and single residues. We herein review, the therapeutic agents targeting the potential biomarkers in light of new molecular targeted therapies. In the final analysis, this review suggests that the development of improved target-specific personalized therapies can combat HNC's global plight.

An in vitro evaluation of zinc silicate fortified chitosan scaffolds for bone tissue engineering.

Tissue engineering aims at replacement, repair, and regeneration of tissues by a combination of scaffolds, growth factors, and cells. In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications. The scaffolds consisted of chitosan (CS) incorporated with mZS at 0.1, 0.2, 0.3, and 0.5 wt%. The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM). The incorporation of mZS was confirmed using Energy dispersive x-ray (EDS) analysis. Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds. The scaffolds also displayed antibacterial action against Staphylococcus aureus. Cellular attachment within the scaffold network established biocompatibility of the material. Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization. Therefore, fabricated scaffolds exhibit exceptional features and have the potential to serve as an implant for BTE applications.

Nanomaterial based gene delivery: a promising method for plant genome engineering.

Nanomaterials have attracted considerable attention from researchers in recent years due to their unique architecture and small dimensions. Significant progress has been made in the therapeutics, diagnostics, and delivery of biomolecules in animal cells. However, nanotechnology is still in its infancy in plant science. Nanotechnology offers tremendous opportunities for crop improvement and would make significant contributions to increase agricultural productivity. There are several reports where nanomaterial-induced improvement of the agronomic traits has been successfully achieved. However, very little is known about the interactions of nanomaterials with plant cells and the mechanism of internalization and delivery of biomolecules using nanoparticles as a carrier. Due to the presence of the cell wall, the delivery of biomolecules such as nucleic acids is a major challenge, which limits the application of nanomaterials in genetic engineering-mediated crop improvement. However, in recent years, the use of various nanomaterials like carbon nanotubes, magnetic nanoparticles, mesoporous silica nanoparticles, etc. for nucleic acid delivery in plant cells has been reported as proof of concept. Here, we intend to update researchers about the use of various nanomaterials as a novel gene delivery tool for plant genetic engineering. This review also explores the progress made in nanoparticle-mediated nucleic acid delivery in plant cells and their role in plant genome engineering.

UV Lithography-Assisted Fabrication of Low-Cost Copper Electrodes Modified with Gold Nanostructures for Improved Analyte Detection.

An in-house UV lithography setup has been optimized to fabricate low-cost disposable electrochemical sensing Cu electrodes using a copper clad board. In view of the high oxidation probability of copper, the low-cost electrodes were modified using different gold nanostructures and a conducing polymer PEDOT:PSS to attain maximal signal output and improved shelf-life. Zero-dimensional (0D) gold nanoparticles (∼40 nm) and three-dimensional (3D) gold nanoflowers (∼38 nm) mixed with PEDOT:PSS were used as signal-enhancing conductors for the ultrasensitive detection of our model contaminant, methylene blue dye (MB). The bare copper electrode was sensitive to MB, linearly within the range of 4-100 µM, with a limit of detection of 3.49 µM. While for gold nanoparticle-PEDOT:PSS-modified electrode, the sensitivity of the electrode was found to increase linearly in the range of 0.01-0.1 µM, and for gold nanoflowers-PEDOT:PSS, the sensitivity achieved was 0.01-0.1 µM with the LOD as 0.0022 µM. For a PEDOT:PSS-modified Cu electrode, used as a comparative to study the contributing role of gold nanostructures towards improved sensitivity, the linearity was found to be in the range of 0.1-1.9 µM with the LOD as 0.0228 µM. A 6 times improvement in signal sensitivity for the nanoflower-PEDOT:PSS electrode compared to the nanoparticle-PEDOT:PSS-modified electrode indicates the influence of nanoparticle shape on the electrode efficiency. 3D gold nanoflowers with a large surface area-to-volume ratio and a high catalytic activity prove to be a superior choice for electrode modification.

Growth Kinetics of Gold Nanoparticle Formation from Glycated Hemoglobin.

Gold nanostructures have always been a subject of interest to physicists, chemists, and material scientists. Despite the extensive research associated with gold nanoparticles, their actual formation mechanism is still debatable. The nanoscale rearrangements leading to the formation of gold nanostructures of definite size and shape are contradictory. The study presented in here details out a mechanism for gold nanoparticle formation in the presence of a biological template. The kinetics of gold nanostructure formation was studied using glycated hemoglobin as a biological template as well as the reducing agent. Particle formation was studied in a time- and temperature-dependent manner using different biophysical techniques. Here, we report for the first time spontaneous formation of gold nanoflowers which gradually dissociates to form smaller spherical particles. In addition, our experiments conclusively substantiate the existing postulations on gold nanoparticle formation from relatively larger precursor structures of gold and contradict with the popular nucleation growth mechanism.