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INTRODUCTION: Oxytropis racemosa Turcz is an important minority medicine that is used mainly to improve children's indigestion, especially in inner Mongolia and Tibet. Previous studies indicated that the characteristic constituents of this plant are acylated flavonoids. OBJECTIVE: Rapidly identify the characteristic chemical constituents of O. racemosa by high-performance liquid chromatography-diode array detection-electrospray ionisation/multi-stage mass spectrometry (HPLC-DAD-ESI/MS(n) ) and suggest a useful method to control the quality of this medicinal plant. METHODS: In the HPLC fingerprint, 32 flavonoids were tentatively identified by a detailed analysis of their mass spectra, UV spectra and retention times. Furthermore, 13 flavonoids were confirmed by comparison with previously isolated compounds obtained from O. racemosa. RESULTS: In total, 32 flavonoids, including 13 flavonoids with 3-hydroxy-3-methylglutaric acid (HMG) moieties and four flavonoids with 3-malonyl moieties, were identified in the extract of O. racemosa. Among the compounds identified, 10 were characterised as new compounds for their particular acylated sugar moieties. CONCLUSIONS: The method described is effective for obtaining a comprehensive phytochemical profile of plants containing unstable acylated flavonoids. The method is also useful for constructing the chromatographic fingerprint of the minority medicine -O. racemosa Turcz for quality control.
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Cromatografía Líquida de Alta Presión/métodos , Flavonoides/análisis , Glucósidos/análisis , Oxytropis/química , Espectrometría de Masa por Ionización de Electrospray/métodos , China , Flavonoides/química , Flavonoides/aislamiento & purificación , Glucósidos/química , Glucósidos/aislamiento & purificación , Estructura Molecular , Plantas Medicinales/química , Reproducibilidad de los Resultados , Espectrofotometría UltravioletaRESUMEN
Potentilin A (1), a rare diflavonol ester of mu-truxinic acid and a new normonoterpenoid, 2,6-dimethyl-2,3-dihydro-4-oxo-4H-pyran-2-acetic acid (2), was isolated from Potentilla anserina, together with 19 known flavonol glycosides (3-21) and 2 known monterpenoids (22,23). Their structures were elucidated by means of UV, IR, HR-ESI-MS, 1D and 2D NMR spectroscopic data.
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Flavonoles/aislamiento & purificación , Glicósidos/aislamiento & purificación , Monoterpenos/aislamiento & purificación , Potentilla/química , Flavonoles/química , Glicósidos/química , Estructura Molecular , Monoterpenos/química , Resonancia Magnética Nuclear Biomolecular , Rizoma/químicaRESUMEN
OBJECTIVE: To study the HLA-DRB1 genetic polymorphism in the Ewenki of Inner mongolia. METHODS: HLA-DRB1 allele polymorphisms in 84 normal Ewenki were determined by PCR with sequencing based typing (SBT). RESULTS: Twenty-five HLA-DRB1 alleles were observed. The allele frequency of HLA-DRB1*03011(14.88%) is the highest; the allele frequencies of HLA -DRB1*09012 (13.69%), DRB1*07011(8.92%), DRB1*04011(9.52%) and DRB1*12011(8.33%) are lower. CONCLUSION: The distribution of HLA-DRB1 alleles frequencies in normal Ewenki from Inner Mongolia exhibits a unique profile, which is of important reference value for studies on anthropology and related illnesses in Ewenki population.
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Pueblo Asiatico/genética , Antígenos HLA-DR/genética , Polimorfismo Genético , China/etnología , Etnicidad , Cadenas HLA-DRB1 , HumanosRESUMEN
OBJECTIVE: To clarify the distribution of genetic polymorphism of D3S1358, D13S317, D5S818, D6S1043, D2S1772, D7S3048 loci of the Mongolian population in Ximeng pastoral area and construct the relevant genetic database. METHODS: Multiplex PCR and polyacrylamide gel electrophoresis were used to investigate the polymorphism of 6 short tandem repeat (STR) loci in 286 individuals of the Mongolian population. RESULTS: In this study, 6, 9, 8, 11, 14, 11 alleles were observed at the 6 STR loci respectively. The genotypes distributions in Mongolian population were in accordance with Hardy-Weinberg equilibrium (P>0.05), the cumulative expected heterozygosities (H), discriminating probability (DP) and the polymorphism information contents (PIC) for the 6 loci were 0.9998, 09999, 0.9998 respectively. These data were compared with those of the Han population. The results showed there were significant difference in D3S1358, D13S317, D5S818, D2S1772, D7S3048 loci between the Mongolian population and Han population (P<0.05). However, no significant difference in D6S1043 locus was seen between the two populations (P>0.05). CONCLUSION: The results demonstrate that these 6 STR loci can serve as genetic marks and provide valuable data which are beneficial to studying the population genetics and ethnology.
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Repeticiones de Microsatélite/genética , Polimorfismo Genético , China , Humanos , Mongolia/etnología , Reacción en Cadena de la PolimerasaRESUMEN
OBJECTIVE: To understand the allele structure and genetic polymorphism at D3S1358, D13S317, D5S818 short tandem repeats (STRs) loci in Nongqu Mongolian of China, and to construct a preliminary database. METHODS: The allele frequencies of the three STRs loci in 291 unrelated individuals from Nongqu Mongolian were analyzed by polymerase chain reaction and polyacrylamide gel electrophoresis. RESULTS: Six, ten, and eight alleles were observed at D3S1358, D13S317, D5S818, respectively, and all 3 loci met Hardy-Weinberg equilibrium. The statistical analysis of 3 STR loci showed the heterozygosity >or=0.7332, the polymorphic information content >or=0.6884; the combined discrimination power and the probabilities of paternity exclusion were 0.9991 and 0.9806 respectively. CONCLUSION: All three of the loci in this study were found to have high heterozygosity and polymorphic information content, so they could provide useful markers for genetic purposes. These results could serve as valuable data to enrich the Mongolian genetic database and play an important role in Chinese population genetic application.