Effect of chronic FSH administration on ovarian follicular development, ovulation rate and corpora lutea formation in sheep.

This study in ewes examined the effects on ovarian function of a pulsatile regimen of ovine FSH (NIADDK-oFSH-17) administered over a 24- to 28-day period beginning on day 1 of the oestrous cycle (day 0 = oestrus). The FSH (1.66 micrograms or 5.00 micrograms) was administered i.v. over a 1-min interval once every hour throughout the treatment period. In other ewes ovine LH (NIDDK-oLH-23) was administered (10 micrograms once every 2 h) for 24-28 days together with oFSH (1.66 micrograms/h). Compared with untreated controls (n = 19 ewes), FSH alone at both doses (n = 10 ewes/dose) as well as the FSH + LH treatment (n = 10) led to significant increases in the plasma concentrations of FSH (P < 0.01), ovarian weight (P < 0.05) and ovulation rate (P < 0.01) but there was no change in the mean weight of individual corpora lutea (CL). Exogenous FSH at the high but not the low dose alone or with LH stimulated a significant overall increase in plasma inhibin concentrations (P < 0.05). The geometric mean (and 95% confidence limits) ovulation rates in the high FSH (i.e. 5.00 micrograms/h), low FSH (i.e. 1.66 micrograms/h), low FSH + LH, and control treatment groups were 15.3 (9.3, 24.8), 3.7 (2.1, 6.0), 3.7 (2.5, 5.8) and 1.4 (1.2, 1.7) respectively. The FSH or FSH + LH treatments did not alter the total numbers of antral follicles (> or = 1 mm diameter). However, the high but not the low FSH or low FSH + LH treatment led to significant increases in the mean numbers of large follicles (i.e. > 4.5 mm diameter; P < 0.01) and a higher proportion of non-atretic antral follicles. Highly significant linear relationships were found between the mean plasma concentrations of FSH or inhibin and the ovulation rate (FSH: r = 0.74, P < 0.0001; inhibin: r = 0.93, P < 0.0001). Highly significant linear relationships were also found between the plasma concentrations of FSH or inhibin and the number of large follicles (i.e. > 4.5 mm diameter; FSH, r = 0.78, P < 0.0001; inhibin, r = 0.80, P < 0.0001) and between the plasma concentrations of inhibin and the number of granulosa cells in large follicles (r = 0.78, P < 0.0001). After the high FSH but not the low FSH treatment there were significant increases in both FSH- and LH-induced responsiveness in granulosa cells with respect to cyclic AMP synthesis in vitro.(ABSTRACT TRUNCATED AT 400 WORDS)

[Congenital cholesteatoma of the middle ear]. / Colesteatoma congénito de oído medio.

The AA. inform about 8 patients suffering from congenital cholesteatoma of the tympanomastoidal cleft. They underline the difficulties for achieving a correct diagnosis when the eardrum remains intact and remark the need to keep in mind this process for differential diagnosis of the conductive hypoacusis in childhood. As a matter of fact the AA. emphasize the few cases of recurrences after surgery, in contrast with that of relapses in child's acquired cholesteatoma.

[Cytogenetic study in otosclerosis]. / Estudio citogenético en la otoesclerosis.

In 50 patients (32 females and 18 males) with otosclerosis who underwent stapedectomy, a cytogenetic study of chromosomal abnormalities was made. No description of G banding in patients with otosclerosis was found in the literature. No chromosomal abnormalities were found in the karyotype using this technique.

[Laryngeal paraganglioma: a case report]. / Paraganglioma laríngeo: a propósito de un caso.

A case of laryngeal paraganglioma in a 25-year-old female is reported. The site of the tumor was the left aryepiglotic fold and ventricular band. The diagnosis was made by histology and immunohistochemistry medial thyrotomy was performed in order to remove a bilateral carotid paraganglioma. We analyzed 76 cases reported in the world literature, clinical, histological, prognostic, and therapeutic factors in laryngeal paraglioma.

Plasma gonadotropin concentrations and ovarian characteristics in Inverdale ewes that are heterozygous for a major gene (FecX1) on the X chromosome that influences ovulation rate.

Ewes heterozygous (I+) for the Inverdale prolificacy gene (FecX1) located on the X chromosome have ovulation rates approximately 1.0 times higher than noncarriers (++). The aims of this study were to examine, in I+ and ++ ewes, the peripheral plasma concentrations and/or ovarian secretion rates of FSH, LH, inhibin, estradiol, and progesterone during anestrus and the estrous cycle and after ovariectomy. Also examined were aspects of ovarian morphology and functions of granulosa cells in vitro. No FecX1-specific differences were noted for the ovarian hormones or for FSH or LH. However, I+ animals contained significantly more ovarian antral follicles (p < 0.05) and their granulosa cells had a higher mean LH responsiveness in vitro with respect to cAMP synthesis at smaller follicular diameters relative to ++ ewes (I+ = > 2.5 mm; ++ = > 4.5 mm). Moreover, nonatretic follicles in I+ animals compared to the ++ genotype contained fewer granulosa cells and smaller CL. Although the I+ animals had a higher ovulation rate than the controls (p < 0.05), the total weight of CL was not different between the genotypes. These findings suggest that the FecX1 gene affects ovarian function without altering ovarian hormone secretion. The findings also suggest that there are no FecX1-specific differences in the mean concentrations of gonadotropins, although further studies on temporal changes in gonadotropin secretion are warranted.

Indicadores de actividad amilasa en la enfermedad parotídea. Parotiditis crónica recurrente infantil / Amylase activity markers for parotid disease: infant recurrent chronic parotiditis

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