[Restoration of root canal treated teeth using carbon fiber posts]. / Gyökérkezelt fogak rekonstrukciója szénszálas müanyag csapok alkalmazásával.

The restoration of root canal treated teeth--because of the significant loss of tooth structure--is often achieved with post and core. However, post may generate stresses which lead to vertical root fracture and loss of the tooth. Since post design, material used and the post space preparation has significant influence on vertical fracture prevalence, broad investigation is in progress to find out the optimum procedure. During the last decade new prefabricated passive posts were introduced for postendodontic restorations. In order to collect information clinical trials have been performed on the reconstruction of root canal treated teeth using carbon fibe posts (COMPOSIPOST, RTD, France; C-POST, Bisco, USA). Adhesive technique was applied to cement posts in the root canal and for composite core reconstruction. The physical properties of carbon fiber posts and the composite core are very close to those of dentin. Post application is simple, does not require special skill and for the patients means minimum hazard. The position of the posts was controlled by radiography. During the 24 months observation period no failure was registered in patients treated (n = 55). Hence, we attribute our good results to the homogeneous reconstruction of the teeth. This procedure seems to be a good alternative to traditional cast metal dowel/cores or metal prefabricated posts.

Comparison of commercially available target enrichment methods for next-generation sequencing.

Isolating high-priority segments of genomes greatly enhances the efficiency of next-generation sequencing (NGS) by allowing researchers to focus on their regions of interest. For the 2010-11 DNA Sequencing Research Group (DSRG) study, we compared outcomes from two leading companies, Agilent Technologies (Santa Clara, CA, USA) and Roche NimbleGen (Madison, WI, USA), which offer custom-targeted genomic enrichment methods. Both companies were provided with the same genomic sample and challenged to capture identical genomic locations for DNA NGS. The target region totaled 3.5 Mb and included 31 individual genes and a 2-Mb contiguous interval. Each company was asked to design its best assay, perform the capture in replicates, and return the captured material to the DSRG-participating laboratories. Sequencing was performed in two different laboratories on Genome Analyzer IIx systems (Illumina, San Diego, CA, USA). Sequencing data were analyzed for sensitivity, specificity, and coverage of the desired regions. The success of the enrichment was highly dependent on the design of the capture probes. Overall, coverage variability was higher for the Agilent samples. As variant discovery is the ultimate goal for a typical targeted sequencing project, we compared samples for their ability to sequence single-nucleotide polymorphisms (SNPs) as a test of the ability to capture both chromosomes from the sample. In the targeted regions, we detected 2546 SNPs with the NimbleGen samples and 2071 with Agilent's. When limited to the regions that both companies included as baits, the number of SNPs was ∼1000 for each, with Agilent and NimbleGen finding a small number of unique SNPs not found by the other.