RESUMEN
This work investigated the feasibility of using feed-forward neural networks for estimation of a state variable in a process with highly non-linear characteristics. A biochemical process was considered where the microorganism Saccharomyces cerevisiae, a yeast, grows in a chemostat on a glucose substrate and produces ethanol as a product of primary energy metabolism. Three state variables for the process are the microbial concentration, substrate concentration and product concentration. The Levenberg-Marquardt Method was used to train the neural networks by minimising the sum of squares of the residuals. The inputs to the networks were the measured variable (product concentration) and the control variable (dilution rate). The output of the network was an estimate for the microbial concentration. Earlier work had shown that system identification of this biochemical process could be performed quite well using feed-forward neural networks. This work demonstrated that state estimation can also be performed successfully using feed-forward neural networks. Knowledge of the process model is not required. The method is simple, reliable and accurate enough for engineering purposes. It can save a lot of expense on sensors, their installation and maintenance.