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Physiol Chem Phys Med NMR ; 22(2): 63-72, 1990.
Artículo en Inglés | MEDLINE | ID: mdl-2100006

RESUMEN

The trifunctional aziridine XAMA-7 (CAS 57116-45-7) has been used to form crosslinks between a deep red-violet copper cluster of the type Cu(I)8Cu(II)6pen12Cl5- (pen=penicillamine) and molecules with biological activity such as d-biotin and proteins. A complex containing biotin, bovine serum albumin and the copper cluster displayed activity toward affinity columns of avidin on Agarose, and the red-violet pigment was immobilized on the gel. This interaction was completely blocked in gels which had been pretreated with d-biotin carboxylic acid. The free and biologically active versions of the cluster have some potential for biomedical applications. For example, the short-lived positron emitter 64Cu (suitable for positron tomography) may be carried in the cluster's structure. The cluster is paramagnetic, but it is a relatively weak effector of water proton spin-lattice relaxation. Other members of this structural group of inorganic compounds may have better magnetic properties, and the crosslinking reaction with aziridines appears to be generally applicable to the group.


Asunto(s)
Biotina , Cobre/química , Penicilamina/química , Aziridinas , Reactivos de Enlaces Cruzados , Portadores de Fármacos , Humanos , Cinética , Imagen por Resonancia Magnética , Penicilamina/síntesis química , Unión Proteica , Albúmina Sérica , Albúmina Sérica Bovina
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