RESUMEN
This immunohistochemical study evaluated the inflammatory infiltrate with its cluster differentiation markers (CD 4, CD 8, CD 20, and CD 68) in aggressive and chronic periodontitis gingival tissues in order to identify the specific cell distribution. Twenty-seven human gingival biopsies were obtained and analyzed. Fourteen patients were suffering from chronic periodontitis and six from aggressive periodontitis; seven patients with healthy gingiva were included as the control group. The specimens were immunohistochemically stained for anti-CD 4 (T helper cells), anti-CD 8 (T cytotoxic/suppressor), anti CD-20 (B plasma cells) and anti CD-68 (macrophages). Chronic periodontitis samples were mainly dominated by CD 4 and CD 8+ cells. On the contrary, in aggressive periodontitis patients all four cell types (CD 4, CD 8, CD 20 and CD 68 + cells, respectively) were remarkably increased. CD 20+ cells were significantly (p < 0.05) more prevalent in aggressive versus chronic periodontitis. The control samples expressed lower CD 4, CD 8, CD 20 and CD 68+ cells confirming a none inflammatory state. The present study demonstrates prevalence for CD 20+ cells in aggressive periodontitis lesions. However, further studies need to be performed to confirm and identify a clear pattern of inflammatory cells and hereafter the mechanisms sustaining the disease.
Asunto(s)
Periodontitis Agresiva/inmunología , Periodontitis Crónica/inmunología , Mediadores de Inflamación/química , Subgrupos Linfocitarios/inmunología , Macrófagos/inmunología , Adulto , Anciano , Antígenos CD/inmunología , Antígenos CD20/inmunología , Antígenos de Diferenciación Mielomonocítica/inmunología , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD8-positivos/inmunología , Estudios de Casos y Controles , Femenino , Humanos , Técnicas para Inmunoenzimas , Masculino , Persona de Mediana Edad , Células Plasmáticas/inmunología , Estadísticas no Paramétricas , Adulto JovenRESUMEN
O principal parâmetro para avaliação do sucesso dos implantes osseointegrados é baseado no percentual do contato do tecido ósseo à superfície do implante. Este processo depende de vários fatores que vão desde a inserção do implante no leito cirúrgico e a formação do coágulo sanguíneo a todos os eventos celulares relacionados à biologia óssea peri-implantar. Logo, o objetivo deste estudo foi avaliar, de maneira sistemática, a sequência de eventos celulares assim como os fatores que influenciam a qualidade e a quantidade de contato do tecido ósseo humano a superfície do implante e suas consequências clínicas.