Genes coding for histone proteins in man are located on the distal end of the long arm of chromosome 7.

Tritium-labeled complementary RNA's to two cloned sea urchin DNA sequences, one coding for histones H1, H2B, and H4 and the other for H2A and H3, were hybridized in situ to high resolution human chromosomes. Evidence is presented showing that the histone genes in man are localized in bands q32-36 on the long arm of chromosome 7.

High-resolution chromosome analysis in clinical medicine.

The field of human cytogenetics has expanded considerably in the past few years due, to a large extent, to the application of the metaphase banding techniques. Among many advances, the chromosomal abnormalities involved in over thirty newly discovered syndromes have been defined and consistent chromosome defects have been observed in several neoplasias. Very recent improvements in culture techniques through cell synchronization and in chromosome treatments, such as the use of minimal exposure to colcemid and/or the use of agents that inhibit chromosome condensation, allow for the routine utilization of highly elongated and finely banded prophase chromosomes. These new techniques are being used clinically to uncover previously undetectable chromosome defects, to localize the exact break points involved in numerous duplication-deficiencies known in man, and to establish possible phenotype-genotype relationships at a refined level.

Self-regulation and external reinforcement in problem-solving strategies of black adolescents.

Examined the relationships among self-regulatory behaviors, perceptions of social reinforcement from significant persons, and the problem-solving performance of black adolescents (N = 33). The components of self-regulatory processes--self-reinforcement, self-evaluation and self-monitoring--were interrelated highly. Ss' perception of neither positive nor negative social reinforcement was related significantly to problem-solving performance. Discussion centered around the effects of self-responsibility and affective factors in the achievement orientation of minority children.

Two new Escherichia coli o antigens, o162 and o163, and one new h antigen, h56. withdrawal of h antigen h50.

Three E. coli strains representing strains from faeces of normal persons were established as antigenic test strains of two new O antigens, O162 and O163, and one new H antigen, H56.

The isolation of Escherichia coli from a poultry packing station and an abattoir.

The distribution and serotype of strains of Escherichia coli from a poultry packing station and an abattoir are described. The results indicated that animal faecal strains contaminated the environment and the animal carcasses.Using 150 O antisera, a high proportion of the E. coli strains were non-typable. This suggests that the serotype distribution of E. coli in animals is different from that in man.Strains with single antigenic differences were isolated, and the possibility of genetic transfer of these antigenic structures is suggested.

Escherichia coli serotype distribution in man and animals.

The serotype distributions of Escherichia coli isolated from animal faeces, human faeces and from meat have been compared. There were marked differences in serotype distribution in strains from man and animals. The meat strains generally resembled animal strains. The results suggest either that animal strains of E. coli are not reaching the general human population outside hospital to any great extent, or, if they do so, are failing to implant in the bowel.

Further studies of Escherichia coli in babies after normal delivery.

Previous work showed that on the basis of O serotyping alone of Escherichia coli, the majority of babies acquired the same O serotype as was found in the stools of their respective mothers. Further characterization of the E. coli by H serotyping, determination of their antibiotic resistance and ability to ferment six carbohydrates showed that in the majority of cases the previous results were confirmed. In a minority of cases this further testing showed that the strains were not identical. In some instances a number of strains isolated from the same pair showed different combinations of the markers used.

Spread of Escherichia coli colonizing newborn babies and their mothers.

Most babies are colonized by the predominant strains of Escherichia coli present in their own mother's faecal flora. Those babies who did not acquire their maternal faecal flora acquired strains of E. coli belonging to a small number of the possible serotypes. Moreover, the same serotypes were found in several babies and other mothers, suggesting spread within the ward. These few strains included some of the O groups which had previously commonly been found as urinary pathogens. These strains may have increased potentialities for colonization of human bowel. Antigenic and biochemical variation was observed among the strains.