RESUMEN
INTRODUCTION: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESULTS: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples. CONCLUSIONS: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.
Asunto(s)
Artemisininas/farmacología , Resistencia a Medicamentos/genética , Malaria Falciparum/parasitología , Mutación/genética , Plasmodium falciparum/genética , Proteínas Protozoarias/genética , Genotipo , Humanos , Fenotipo , Plasmodium falciparum/efectos de los fármacosRESUMEN
INTRODUCTION: The mosquito Aedes aegypti has evolved resistance to pyrethroid insecticides. The present study evaluated Ae. aegypti from Goiânia for the resistant phenotype and for mutations associated with resistance. METHODS: Insecticide dose-response bioassays were conducted on mosquitoes descended from field-collected eggs, and polymerase chain reaction (PCR) was used to genotype 90 individuals at sites implicated in pyrethroid resistance. RESULTS: All mosquito populations displayed high levels of resistance to deltamethrin, as well as high frequencies of the 1016Ile kdr and 1534Cys kdrmutations. CONCLUSIONS: Aedes aegypti populations in the Goiânia area are highly resistant to deltamethrin, presumably due to high frequencies of kdr(knockdown-resistance) mutations.
Asunto(s)
Aedes/efectos de los fármacos , Aedes/genética , Resistencia a los Insecticidas/genética , Insecticidas , Mutación/genética , Nitrilos , Piretrinas , Aedes/clasificación , Animales , Bioensayo , Brasil , Femenino , Genotipo , Fenotipo , Reacción en Cadena de la PolimerasaRESUMEN
Abstract INTRODUCTION Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESULTS: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples. CONCLUSIONS: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.