RESUMEN
It has been shown that most of Drosophila melanogaster mutant lines obtained as a result of X-rays irradiation (XI) as well as of the combined action of XI and some chemical agents are characterized by decreased indexes of average (7-40 %) and maximal (1-35 %) life span. Insertion-excision processes at the instable genes white and cut are among the reasons of decreased vitality and shortened life span in induced mutants. Collection of neurodegenerative mutants has been obtained under the influence of ENU. Fast dying of flies and decreased vitality correlated with time point of neurodegenerations in brain structure.
Asunto(s)
Drosophila melanogaster , Longevidad , Mutación , Animales , Encéfalo/efectos de los fármacos , Encéfalo/patología , Encéfalo/efectos de la radiación , Cafeína/toxicidad , Drosophila melanogaster/efectos de los fármacos , Drosophila melanogaster/genética , Drosophila melanogaster/crecimiento & desarrollo , Drosophila melanogaster/efectos de la radiación , Etilnitrosourea/toxicidad , Genes de Insecto/efectos de los fármacos , Genes de Insecto/efectos de la radiación , Dosificación Letal Mediana , Longevidad/efectos de los fármacos , Longevidad/genética , Longevidad/efectos de la radiación , Cromosoma X/efectos de los fármacos , Cromosoma X/genética , Cromosoma X/efectos de la radiación , Rayos X/efectos adversosRESUMEN
The genetic instability of Drosophila melanogaster genes induced by the mobile genetic elements is reviewed. The main attention is paid to genetic instability depended on types of crossing. Data on the possibility of genetic instability induction by the chemical and physical (X-rays, heat-shock) agents and their complex effect are cited. It was shown that a number of agents which cause mutagenic effect realize their action by involving of mobile genetic elements.
Asunto(s)
Drosophila melanogaster/genética , Genes de Insecto/genética , Variación Genética/genética , Secuencias Repetitivas Esparcidas/genética , Animales , Intercambio Genético/efectos de los fármacos , Intercambio Genético/genética , Intercambio Genético/efectos de la radiación , Drosophila melanogaster/efectos de los fármacos , Drosophila melanogaster/efectos de la radiación , Genes de Insecto/efectos de los fármacos , Genes de Insecto/efectos de la radiación , Variación Genética/efectos de los fármacos , Variación Genética/efectos de la radiación , Secuencias Repetitivas Esparcidas/efectos de los fármacos , Secuencias Repetitivas Esparcidas/efectos de la radiaciónRESUMEN
The influence of mitomycin C on the genetic stability of Drosophila laboratory lines was investigated. This mutagen caused one-locus and multi-loci mutations with high frequencies (approximately 10(-2), 10(-3)) in the first and the second generations. It is locus-specific mutagenesis. In situ hybridization experiments indicated the existence of primary and new sites of location of mobile genetic elements mdg 1, mdg 2, mdg 4. This instability was caused by activation of transposition and recombination.
Asunto(s)
Drosophila melanogaster/efectos de los fármacos , Drosophila melanogaster/genética , Mitomicina/farmacología , Mutación , Animales , ADN/genética , Elementos Transponibles de ADN/efectos de los fármacos , Elementos Transponibles de ADN/genética , Femenino , Genotipo , Hibridación in Situ , Masculino , Cromosoma X/efectos de los fármacos , Cromosoma X/genéticaRESUMEN
Under the influence of ethyl methanesulfonate the series of both morphological and structural mutants with different types of brain changes has been obtained in Drosophila melanogaster Oregon R strain. In the future, this collection of mutants will be used in the investigations of genetic control of brain degeneration and possible ways of brain regeneration.