RESUMEN
In the realm of mmWave communication and connectivity, integrating chips and antennas into a cohesive system is paramount. Given this, planar antenna arrays have become indispensable. In this article, we introduce a novel antenna array tailored for mmWave applications, characterized by its high directivity. Distinctively, this new array employs a flat-panel radiator, ensuring an augmented gain without necessitating additional superstrate layers. To validate its potency, a 4 × 4 flat-panel array with dimensions of 3.74 λ0 × 3.74 λ0 × 0.106 λ0 at 28 GHz including a ground plane was designed and tested for n257 band. The standalone array element exhibited a bandwidth of 20.6%, centered at 28.5 GHz. Furthermore, a 1 × 16 mmWave feed network was designed and amalgamated with the array elements to assess the comprehensive antenna performance. The measured peak gain of 21.3 dBi at 28.5 GHz was observed with the measured half power beamwidth of 15° while the gain variation within the operation band was less than 3 dB.
RESUMEN
Existing methods to enrich target regions of genomic DNA based on PCR, hybridization capture, or molecular inversion probes have various drawbacks, including long experiment times and low throughput and/or enrichment quality. We developed CRISPR-Cap, a simple and scalable CRISPR-based method to enrich target regions of dsDNA, requiring only two short experimental procedures that can be completed within two hours. We used CRISPR-Cap to enrich 10 target genes 355.7-fold on average from Escherichia coli genomic DNA with a maximum on-target ratio of 81% and high enrichment uniformity. We also used CRISPR-Cap to measure gene copy numbers and detect rare alleles with frequencies as low as 1%. Finally, we enriched coding sequence regions of 20 genes from the human genome. We envision that CRISPR-Cap can be used as an alternative to other widely used target-enrichment methods, which will broaden the scope of CRISPR applications to the field of target enrichment field.