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VITAS, a sensitive in vivo selection assay to discover enzymes producing antiviral natural products.
Chem Commun (Camb)
; 59(36): 5419-5422, 2023 May 02.
Artículo
en Inglés
| MEDLINE
| ID: mdl-37066494
RESUMEN
To discover new broad-spectrum antiviral nucleotide analogues from natural resources or through protein engineering, we have developed a sensitive in vivo selection assay named Viral polymerase-Inhibition Toxin-Associated Selection (VITAS). We show that the assay works with enzymes from three Kingdoms of life.