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EMBO J ; 38(11)2019 06 03.
Artículo en Inglés | MEDLINE | ID: mdl-31000523

RESUMEN

Myeloid-derived suppressor cells (MDSCs) are immature myeloid cells with strong immunosuppressive activity that promote tumor growth. In this study, we describe a mechanism by which cancer cells control MDSCs in human cancers by upregulating TRF2, a protein required for telomere stability. Specifically, we showed that the TRF2 upregulation in cancer cells has extratelomeric roles in activating the expression of a network of genes involved in the biosynthesis of heparan sulfate proteoglycan, leading to profound changes in glycocalyx length and stiffness, as revealed by atomic force microscopy. This TRF2-dependent regulation facilitated the recruitment of MDSCs, their activation via the TLR2/MyD88/IL-6/STAT3 pathway leading to the inhibition of natural killer recruitment and cytotoxicity, and ultimately tumor progression and metastasis. The clinical relevance of these findings is supported by our analysis of cancer cohorts, which showed a correlation between high TRF2 expression and MDSC infiltration, which was inversely correlated with overall patient survival.


Asunto(s)
Glicocálix/metabolismo , Neoplasias/inmunología , Neoplasias/patología , Proteína 2 de Unión a Repeticiones Teloméricas/fisiología , Escape del Tumor/fisiología , Animales , Células Cultivadas , Femenino , Regulación Neoplásica de la Expresión Génica , Glicocálix/genética , Células HEK293 , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Desnudos , Células Supresoras de Origen Mieloide/metabolismo , Células Supresoras de Origen Mieloide/fisiología , Células 3T3 NIH , Neoplasias/genética , Neoplasias/mortalidad , Telómero/metabolismo , Proteína 2 de Unión a Repeticiones Teloméricas/genética , Escape del Tumor/genética
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