Sensitive sepiolite-carbon nanotubes based disposable electrodes for direct detection of DNA and anticancer drug-DNA interactions.

A new surface based on the natural clay mineral sepiolite and a single-walled carbon nanotubes-modified graphite electrode was developed for the electrochemical detection of DNA, and also for anticancer drug-DNA interactions.

A comparative study of qualitative and quantitative methods for the assessment of adhesive remnant after bracket debonding.

The amount of the residual adhesive after bracket debonding is frequently assessed in a qualitative manner, utilizing the adhesive remnant index (ARI). This study aimed to investigate whether quantitative assessment of the adhesive remnant yields more precise results compared to qualitative methods utilizing the 4- and 5-point ARI scales. Twenty debonded brackets were selected. Evaluation and scoring of the adhesive remnant on bracket bases were made consecutively using: 1. qualitative assessment (visual scoring) and 2. quantitative measurement (image analysis) on digital photographs. Image analysis was made on scanning electron micrographs (SEM) and high-precision elemental maps of the adhesive remnant as determined by energy dispersed X-ray spectrometry. Evaluations were made in accordance with the original 4-point and the modified 5-point ARI scales. Intra-class correlation coefficients (ICCs) were calculated, and the data were evaluated using Friedman test followed by Wilcoxon signed ranks test with Bonferroni correction. ICC statistics indicated high levels of agreement for qualitative visual scoring among examiners. The 4-point ARI scale was compliant with the SEM assessments but indicated significantly less adhesive remnant compared to the results of quantitative elemental mapping. When the 5-point scale was used, both quantitative techniques yielded similar results with those obtained qualitatively. These results indicate that qualitative visual scoring using the ARI is capable of generating similar results with those assessed by quantitative image analysis techniques. In particular, visual scoring with the 5-point ARI scale can yield similar results with both the SEM analysis and elemental mapping.

WDS versus silicon drift detector EDS: a case report for the comparison of quantitative chemical analyses of natural silicate minerals.

Electron probe microanalysis (EPMA) is an essential analytical approach to determine elemental concentrations of various solid specimens quantitatively in mineralogical, petrological and materials research. Either wavelength dispersive X-ray (WDS) or energy dispersive X-ray (EDS) spectrometric techniques can collect the characteristic X-rays generated from each element in the specimen by an incident electron beam in order to define chemical constituents. Although WDS has been the preferred technique because of its higher spectral resolution and ability to detect trace elements, new generation EDS systems with silicon drift detectors (SDD), equipped with thin windows and integrated digital processing electronics, are claimed to approach the WDS throughput. In this study, we compared the analytical capability of a SDD EDS system with respect to WDS equipped systems on natural silicate minerals. For this purpose, natural rock samples, in which the silicate minerals present had already been analysed by various WDS systems, were chosen to compare these results with the ones acquired with a SDD EDS system. SDD EDS yielded satisfactory results for major elements (Na, Mg, Al, Si, K, Ca, Ti, Mn and Fe) compared with the results of the same minerals obtained by various WDS systems.

A rhinocerotid skull cooked-to-death in a 9.2 Ma-old ignimbrite flow of Turkey.

BACKGROUND: Preservation of fossil vertebrates in volcanic rocks is extremely rare. An articulated skull (cranium and mandible) of a rhinoceros was found in a 9.2±0.1 Ma-old ignimbrite of Cappadocia, Central Turkey. The unusual aspect of the preserved hard tissues of the skull (rough bone surface and brittle dentine) allows suspecting a peri-mortem exposure to a heating source. METHODOLOGY/PRINCIPAL FINDINGS: Here we describe and identify the skull as belonging to the large two-horned rhinocerotine Ceratotherium neumayri, well-known in the late Miocene of the Eastern Mediterranean Province. Gross structural features and microscopic changes of hard tissues (bones and teeth) are then monitored and compared to the results of forensic and archaeological studies and experiments focusing on heating effects, in order to reconstruct the hypothetical peri-mortem conditions. Macroscopic and microscopic structural changes on compact bones (canaliculi and lamellae vanished), as well as partial dentine/cementum disintegration, drastic enamel-dentine disjunctions or microscopic cracks affecting all hard dental tissues (enamel, cementum, and dentine) point to continued exposures to temperatures around 400-450°C. Comparison to other cases of preservation of fossil vertebrates within volcanic rocks points unambiguously to some similarity with the 79 AD Plinian eruption of the Vesuvius, in Italy. CONCLUSIONS/SIGNIFICANCE: A 9.2±0.1 Ma-old pyroclastic density current, sourced from the Çardak caldera, likely provoked the instant death of the Karacasar rhino, before the body of the latter experienced severe dehydration (leading to the wide and sustainable opening of the mouth), was then dismembered within the pyroclastic flow of subaerial origin, the skull being separated from the remnant body and baked under a temperature approximating 400°C, then transported northward, rolled, and trapped in disarray into that pyroclastic flow forming the pinkish Kavak-4 ignimbrite ∼30 km North from the upper Miocene vent.

Surface porosity of hand-mixed, syringe-mixed and encapsulated set endodontic sealers.

OBJECTIVE: The objective of this study was to investigate the surface porosity of hand-mixed, syringe-mixed and encapsulated set endodontic sealers using image analysis. STUDY DESIGN: Eight different root canal sealers (AD Seal/Meta Biomed; AH Plus/Dentsply; MM Seal/Micromega; AH Plus Jet/Dentsply; EndoREZ/Ultradent; Epiphany/Pentron; GuttaFlow/Coltène; RealSeal/Sybron Endo) were prepared in accordance with the manufacturer's recommendations and dispensed into plastic rings (n = 10/group). Following set of the specimens, scanning electron photomicrographs of the sealer surfaces were taken at standard magnification. The area fraction and the minimum-maximum diameters of surface pores were calculated on the micrographs using ImageJ open-source image analysis software. Statistical comparisons were made between the sealers with respect to the area fractions and number of pores (Kruskal-Wallis test, P < .05). RESULTS: The endodontic sealers differed significantly in both the percentage of area fraction and pore count (P < .001). The surface porosity of the methacrylate-based sealers Epiphany and RealSeal were significantly lower than those of resin- and silicone-based sealers. With the exception of AD Seal, hand-mixed sealers displayed significantly higher surface porosity than those of syringe-mixed ones (P < .001). The encapsulated sealer GuttaFlow exhibited the highest surface area fraction of pores. CONCLUSION: The mixing method influences the surface porosity of set endodontic sealers.