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Br J Nutr ; 112(4): 573-82, 2014 Aug 28.
Artículo en Inglés | MEDLINE | ID: mdl-24870697

RESUMEN

The consumption of lipids and simple sugars induces an inflammatory response whose exact molecular trigger remains elusive. The aims of the present study were to investigate (1) whether inflammation induced by a single high-energy, high-fat meal (HFM) is associated with endoplasmic reticulum stress (ERS) in peripheral blood mononuclear cells (PBMC) and (2) whether these inflammatory and ERS responses could be prevented by the chemical chaperone ursodeoxycholic acid (UDCA). A total of ten healthy lean men were recruited to a randomised, blind, cross-over trial. Subjects were given two doses of placebo (lactose) or UDCA before the consumption of a HFM (6151 kJ; 47·4 % lipids). Blood was collected at baseline and 4 h after the HFM challenge. Cell populations and their activation were analysed using flow cytometry, and plasma levels of inflammatory cytokines were assessed by ELISA and Luminex technology. Gene expression levels of inflammatory and ERS markers were analysed in CD14⁺ and CD14⁻ PBMC using quantitative RT-PCR. The HFM induced an increase in the mRNA expression levels of pro-inflammatory cytokines (IL-1ß, 2·1-fold; IL-8, 2·4-fold; TNF-α, 1·4-fold; monocyte chemoattractant protein 1, 2·1-fold) and a decrease in the expression levels of miR181 (0·8-fold) in CD14⁺ monocytes. The HFM challenge did not up-regulate the expression of ERS markers (XBP1, HSPA5, EDEM1, DNAJC3 and ATF4) in either CD14⁺ or CD14⁻ cell populations, except for ATF3 (2·3-fold). The administration of UDCA before the consumption of the HFM did not alter the HFM-induced change in the expression levels of ERS or inflammatory markers. In conclusion, HFM-induced inflammation detectable on the level of gene expression in PBMC was not associated with the concomitant increase in the expression levels of ERS markers and could not be prevented by UDCA.


Asunto(s)
Inmunidad Adaptativa , Dieta Alta en Grasa/efectos adversos , Estrés del Retículo Endoplásmico , Hiperfagia/inmunología , Inmunidad Innata , Leucocitos Mononucleares/inmunología , Comidas , Inmunidad Adaptativa/efectos de los fármacos , Adulto , Antiinflamatorios/farmacología , Biomarcadores/sangre , Biomarcadores/metabolismo , Colagogos y Coleréticos/farmacología , Estudios Cruzados , Citocinas/sangre , Citocinas/genética , Citocinas/metabolismo , Chaperón BiP del Retículo Endoplásmico , Estrés del Retículo Endoplásmico/efectos de los fármacos , Ingestión de Energía , Regulación de la Expresión Génica/efectos de los fármacos , Humanos , Hiperfagia/sangre , Hiperfagia/metabolismo , Inmunidad Innata/efectos de los fármacos , Leucocitos Mononucleares/efectos de los fármacos , Leucocitos Mononucleares/metabolismo , Receptores de Lipopolisacáridos/sangre , Receptores de Lipopolisacáridos/metabolismo , Masculino , MicroARNs/metabolismo , Periodo Posprandial , Ácido Ursodesoxicólico/farmacología
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