RESUMEN
OBJECTIVE: To investigate the effect of aqueous extract of Helicteres angustifolia (AEHA) on rats with ulcerative colitis (UC) and its mechanism. METHODS: SD rats were randomly divided into 6 groups normal control group, the model group, SASP group and 21.6, 10.8, 5.4 g/kg AEHA group with 10 rats in each group. UC was induced by clyster with 2, 4,6-trinitrobenzesulphonic acid (TN-BS) in rats. Rats were given AEHA by gavage for 3 weeks, and sacrificed at day 22nd to determine the expressions of IL-10, IL-6 and TNF-alpha in blood. Colon tissue pathological changes were investigated. RESULTS: Compared with the control group, the expression of IL-10 was increased, the expressions of IL-6 and TNF-alpha were decreased in AEHA treatment groups. The colon tissue damages and the symptoms of UC rats were improved. CONCLUSION: AEHA can keep balance of inflammatory factors in blood of UC rats, and improve it's colon tissue damages and symptoms.
Asunto(s)
Antiinflamatorios no Esteroideos/uso terapéutico , Colitis Ulcerosa/tratamiento farmacológico , Medicamentos Herbarios Chinos/uso terapéutico , Malvaceae/química , Animales , Antiinflamatorios no Esteroideos/administración & dosificación , Colitis Ulcerosa/inducido químicamente , Colitis Ulcerosa/metabolismo , Colon/efectos de los fármacos , Colon/metabolismo , Colon/patología , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Femenino , Interleucina-10/sangre , Interleucina-10/metabolismo , Interleucina-6/sangre , Interleucina-6/metabolismo , Masculino , Fitoterapia , Raíces de Plantas/química , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Factor de Necrosis Tumoral alfa/sangre , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
Colorectal cancer (CRC) is one of the most common malignancies and most frequent cause of cancer death worldwide. The activation of both NF-κB and STAT3 signaling and the crosstalk between them play an important role in colorectal tumor. Helicteres angustifolia L. is a type of commonly used Chinese medicinal herb and possesses a wide variety of biological activities. In the present study, we investigate the effects of three triterpenes from H. angustifolia (HT) such as helicteric acid (HA), oleanic acid (OA), and betulinic acid (BA), on inhibiting CRC progression. Our results showed that HT extracts could decrease proliferation and induce apoptosis in HT-29 colorectal cancer cells. Moreover, HT extracts could suppress LPS-triggered phosphorylation of IKK, IκB, and NF-κB, attenuate IL-6-induced phosphorylation of JAK2 and STAT3, and suppress the expression of c-Myc, cyclin-D1, and BCL-xL, the downstream gene targets of NF-κB and STAT3. Therefore, HT extracts showed potent therapeutic and antitumor effects on CRC via inhibiting NF-κB and STAT3 signaling.