Immunofluorescent IgG and IgM antibodies in human sera by enterovirus infections.

IgG and IgM antibodies to selected enterovirus serotypes (P2, CB 1-5, E2, 4, 20), to prototype collection strains and to fresh isolates were examined by immunofluorescence in paired sera of 11 children and 4 adults in whom enterovirus had been isolated, or a rise of specific antibodies had been proved by neutralization test. One six-month- and one seven-month-old child had antibodies of both classes to the isolated virus strain and to poliovirus only. Children from eight month onwards and adults had antibodies to the majority of enterovirus serotypes tested. Among all enteroviruses tested, heterologous reactions were observed not only with IgG but also with IgM class antibodies.

Effect of 60amino-6-deoxy-D-glucose hydrochloride on influenza infection in mice.

A prophylactic effect of 6-amino-6-deoxy-D-glucose hydrochloride (AG) in influenza-infected mice was demonstrated. A dose of 500 mg AG per kg body-weight given intranasally 2000 mouse intranasal LD50) protected 32-40 per cent of the animals from death. The virus titres in the lungs and serum were lower in treated mice than in untreated ones. After AG administration, low interferon titres were demonstrated in the lungs and serum. The group of infected mice, untreated with AG, was examined electron microscopically. Virus was demonstrated on the surface of granular pneumocytes and in the lumen of lung alveoli.

[The clinical picture and etiology of meningoencephalitis in hospitalized patients in 1986]. / Klinický obraz a etiologie meningoencefalitid u hospitalizovaných pacientu v roce 1986.

In 1986 in the CSR 978 cases of acute meningoencephalitis were notified, incl. 184 in the capital. In a group of 129 patients aged 1 to 63 years hospitalized at the First Clinic for Infectious Diseases in Prague-Bulovka (Paediatric Faculty Charles University) in 1986 the authors investigated the clinical course of acute meningoencephalitis in relation to the causal agent of the disease. The aetiological agent was assessed by isolation from cerebrospinal fluid or by isolation from faeces with the corresponding antibody response, or by serological examination only. In children under 15 years of age the etiological agent was assessed in 36%, in adults in 65%. In the investigated group, contrary to previous years (3), tick-borne encephalitis was involved which accounted for the majority of severe cases of the disease. The severe clinical course, manifested at first by symptoms of cerebral oedema with impaired consciousness, is more frequent with advancing age. In adults tick-borne encephalitis had a severe course in one third of the patients. In the other age groups the development was favourable, in particular in the group of children aged 4-10 years. As compared with previous years, there was no fatal case, no permanent paresis as a complication of the disease (2).

[Neurotic symptoms in obese women]. / Neurotické príznaky u obézních zen.

In a group of 129 obese women with a body mass index (BMI) above 25 by means of questionnaire N5 the frequency and intensity of neurotic symptoms was examined. It was revealed that in the sub-group of 61 probands with pathological obesity (BMI above 30) both values were highly significantly higher (p less than 0.01) than in 68 women with simple obesity (BMI 25-30). Among ten most frequent neurotic symptoms in obese women above all depressive and vegetative symptoms predominated.

[Virologic and morphologic study in sudden and unexpected death in children]. / Virologická a morfologická studie u náhle a neocekávane zemrelých detí.

The authors examined a group of 47 children who died at the age of 1-9 months, incl. 19 female and 28 male children. In 23 the death was described as sudden and in 24 as unexpected. From 19 dead children a virus was isolated: in 14 one or two serotypes of enterovirus were found, in two the virus of parainfluenza 3 in two adenoviruses (type 2, 7). A total of 46 viral strains were isolated. By immunofluorescence in tissues antigens of the parainfluenza virus 3 and adenovirus were detected. Immune complexes were not detected in tissues. The distribution of vaccination polioviruses in organs and tissues after a known interval following vaccination was recognized. In some of the infants a possible causal relationship between the virological and morphological findings was found and the authors discuss the possible pathogenetic role of the revealed changes in sudden death.