RESUMEN
The ADMA-like pre-eclampsia in pregnant rats was modeled by daily introduction of L-NAME in a dose of 25 mg/kg for 7 days. L-arginine (200 mg/kg) prevented the development of arterial hypertension and a decrease in placentary microcirculation and microalbuminuria. The possibility of using L-arginine for the prevention of competitive eNOS inhibition by ADMA is discussed.
Asunto(s)
Arginina/administración & dosificación , Endotelio/efectos de los fármacos , Hipertensión/prevención & control , Placenta/efectos de los fármacos , Preeclampsia/tratamiento farmacológico , Albuminuria/prevención & control , Animales , Arginina/uso terapéutico , Presión Sanguínea/efectos de los fármacos , Endotelio/fisiología , Femenino , Humanos , Inyecciones Intraperitoneales , Microcirculación/efectos de los fármacos , Microcirculación/fisiología , NG-Nitroarginina Metil Éster/administración & dosificación , NG-Nitroarginina Metil Éster/efectos adversos , Óxido Nítrico Sintasa de Tipo III/metabolismo , Placenta/irrigación sanguínea , Preeclampsia/inducido químicamente , Preeclampsia/metabolismo , Embarazo , RatasRESUMEN
L-arginine (200 mg/kg), vitamin B(6) (2 mg/kg), and folic acid (0.2 mg/kg) exert a protective effect on endothelial function in L-NAME-induced NO deficiency in male and pregnant female Wistar rats. Combined administration of these agents effectively prevented the development of endothelial dysfunction and L-NAME-induced preeclampsia.
Asunto(s)
Arginina/farmacología , Endotelio/fisiopatología , Ácido Fólico/farmacología , Óxido Nítrico/deficiencia , Placenta/irrigación sanguínea , Vitamina B 6/farmacología , Animales , Arginina/uso terapéutico , Quimioterapia Combinada , Endotelio/efectos de los fármacos , Endotelio/metabolismo , Femenino , Ácido Fólico/uso terapéutico , Masculino , Microcirculación/efectos de los fármacos , NG-Nitroarginina Metil Éster , Óxido Nítrico Sintasa de Tipo III/metabolismo , Placenta/efectos de los fármacos , Placenta/metabolismo , Preeclampsia/inducido químicamente , Preeclampsia/tratamiento farmacológico , Preeclampsia/metabolismo , Embarazo , Ratas , Ratas Wistar , Vitamina B 6/uso terapéuticoRESUMEN
Intragastric methionine (3 g/kg daily for 7 days) elevates homocysteine concentration and increases the endothelial dysfunction coefficient. This protocol of methionine treatment is an adequate model of hyperhomocysteine-induced endothelial dysfunction and can be used for studies of the endothelio- and cardioprotective effects of drugs.
Asunto(s)
Endotelio Vascular/patología , Hiperhomocisteinemia/fisiopatología , Enfermedades Vasculares/fisiopatología , Animales , Endotelio Vascular/efectos de los fármacos , Hiperhomocisteinemia/complicaciones , Hiperhomocisteinemia/tratamiento farmacológico , Metionina/uso terapéutico , Ratas , Enfermedades Vasculares/tratamiento farmacológico , Enfermedades Vasculares/etiologíaRESUMEN
Experimental NO deficiency induced by L-NAME injection led to the development of arterial hypertension, endothelial dysfunction, and cardiomyocyte hypertrophy and reduced blood content of nitrates and nitrites. Impaza, NO donors, activators of NO-synthase, antioxidants, and antihypertensive preparations produced endothelium-protective effect of different degree.
Asunto(s)
Anticuerpos/uso terapéutico , Endotelio Vascular/efectos de los fármacos , Óxido Nítrico/deficiencia , Animales , Antihipertensivos/uso terapéutico , Antioxidantes/uso terapéutico , Hipertensión/inducido químicamente , Hipertensión/tratamiento farmacológico , Masculino , NG-Nitroarginina Metil Éster/farmacología , Nitratos/metabolismo , Donantes de Óxido Nítrico/uso terapéutico , Nitritos/metabolismo , Ratas , Ratas Wistar , Resveratrol , Estilbenos/uso terapéuticoRESUMEN
We studied the effects of antioxidants resveratrol and pQ510 on physiological parameters and the state of endothelial NO-synthase as a marker of the regulatory function of the endothelium in the aorta of rats with modeled arterial hypertension. The antioxidants promoted recovery of stable NO metabolites in rat serum and maintained expression of endothelial NO-synthase at a normal level. These effects were confirmed by correction of blood pressure and endothelium-dependent vascular dilation assessed by endothelial dysfunction coefficient.