RESUMEN
A pattern recognition software PlatProm, which takes into consideration both sequence-specific and structure-specific features in the genetic environment of the promoter sites and identifies transcription start points with a very high accuracy was used to reveal potentially transcribed regions in the genomes of two bacterial species. Along with the expected promoters located upstream from coding sequences PlatProm identified several hundred of very similar signals in other intergenic regions and within coding sequences. Homologous genes of Escherichia coli and Salmonella typhimurium, containing potential promoters on the template strand are suggested as putative targets for regulations by antisense RNA-products (aRNAs).
Asunto(s)
Algoritmos , Mapeo Cromosómico/métodos , Escherichia coli/genética , Genoma Bacteriano/genética , Regiones Promotoras Genéticas/genética , ARN sin Sentido/genética , Salmonella typhimurium/genética , Inteligencia Artificial , Secuencia de Bases , Datos de Secuencia Molecular , Reconocimiento de Normas Patrones Automatizadas/métodos , Análisis de Secuencia de ARN/métodos , Programas InformáticosRESUMEN
A charge-coupled device (CCD) was used to quantitatively detect isotope-labeled ligands bound to a protein microarray. Protein microarrays with protein dots, 10-50 microm in diameter, were fabricated on an aluminized Mylar film using an electrospray deposition technique. Proteins in dots were immobilized by cross-linking in glutaraldehyde vapor. After contact with solutions of isotope-labeled metabolites, the protein microarrays were washed, dried and placed face down onto the surface of a standard B/W video CCD chip with the protective window removed. We show here that such a simple inexpensive CCD detector can be used to quantify distribution of 14C and other radioactive isotopes on microarrays.