RESUMEN
The triallelic pattern of short tandem repeat (STR) is rare; especially, the case where this pattern exists at 4 loci has not been reported. Here, we report the type 1 triallelic patterns at D5S818, D18S51, D6S1043, and FGA from a Chinese family, which were observed during our routine chimerism assays. Before hematopoietic stem cell transplantation, the blood sample of the certain patient was analyzed by performing chimerism analysis. A preliminary STR analysis was also performed on the samples of the patient's parents. STR signal data illustrated that the sum of the peak chart areas of the two types inherited from the father was basically the same as that of the mother, belonging to the type 1 triallelic pattern. In addition, the patient's elder sister's STR result appeared to be normal. Altogether, we presented a pedigree, in which the triallelic pattern was linked by inheritance in the family. This is the first reported case of the triallelic pattern at D5S818, D18S51, D6S1043, and FGA all around the world. We hope that in the future there will be any tools to achieve accurate verification against this possibility.
Asunto(s)
Repeticiones de Microsatélite , Anciano , Frecuencia de los Genes , Humanos , Repeticiones de Microsatélite/genéticaRESUMEN
BACKGROUND: The killer cell immunoglobulin-like receptor (KIR), which mediates the killing function of NK cells, is an attractive candidate for adoptive cellular therapy. The ethnic distribution for China provides a unique opportunity to investigate KIR gene distribution. AIM: The aim of this study was to explore the relationship between population history and the rapidly evolving KIR genetic diversity. SUBJECTS AND METHODS: 8050 Chinese donors from 184 hospitals were included to analyse frequency, haplotype, and B-content data of 16 KIR genes, by PCR-SSP for KIR genotyping. RESULTS: KIR gene carrier frequencies were found similar to those observed in other studies on Han, but different from Thais, Japanese, Africans, and populations of West Eurasian ancestry. High-frequency KIR genotype profiles found in the present population were consistent with other studies on Han populations but different from those conducted on other cohorts. The majority of our cohort carried group A KIR gene motifs. Additionally, populations with similar geographic locations in China were shown clustered together, while Hainan and Xinjiang provinces were slightly separated from these. CONCLUSION: The distribution of KIR genes varies by geographic region, and different ethnic groups may be a confounding factor of KIR diversity.
Asunto(s)
Frecuencia de los Genes , Haplotipos , Receptores KIR/genética , China , Estudios de Cohortes , Heterocigoto , HumanosRESUMEN
Several genes involved in the pathogenesis have been identified, with the human leukocyte antigen (HLA) system playing an essential role. However, the relationship between HLA and a cluster of hematological diseases has received little attention in China. Blood samples (n = 123913) from 43568 patients and 80345 individuals without known pathology were genotyped for HLA class I and II using sequencing-based typing. We discovered that HLA-A*11:01, B*40:01, C*01:02, DQB1*03:01, and DRB1*09:01 were prevalent in China. Furthermore, three high-frequency alleles (DQB1*03:01, DQB1*06:02, and DRB1*15:01) were found to be hazardous in malignant hematologic diseases when compared to controls. In addition, for benign hematologic disorders, 7 high-frequency risk alleles (A*01:01, B*46:01, C*01:02, DQB1*03:03, DQB1*05:02, DRB1*09:01, and DRB1*14:54) and 8 high-frequency susceptible genotypes (A*11:01-A*11:01, B*46:01-B*58:01, B*46:01-B*46:01, C*01:02-C*03:04, DQB1*03:01-DQB1*05:02, DQB1*03:03-DQB1*06:01, DRB1*09:01-DRB1*15:01, and DRB1*14:54-DRB1*15:01) were observed. To summarize, our findings indicate the association between HLA alleles/genotypes and a variety of hematological disorders, which is critical for disease surveillance.
Asunto(s)
Enfermedades Hematológicas , Antígenos de Histocompatibilidad Clase I , Humanos , Frecuencia de los Genes , Alelos , Cadenas beta de HLA-DQ/genética , Cadenas HLA-DRB1/genética , Genotipo , Antígenos de Histocompatibilidad Clase I/genética , Enfermedades Hematológicas/genética , Haplotipos , Predisposición Genética a la EnfermedadRESUMEN
HLA-DQB1*06:475 differs from HLA-DQB1*06:35 by one nucleotide in exon 2.
RESUMEN
HLA-A*30:01:24 differs from HLA-A*30:01:01:01 by one nucleotide in exon 3.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-A , Humanos , Alelos , Análisis de Secuencia de ADN , Antígenos HLA-A/genética , NucleótidosRESUMEN
HLA-C*04:490 differs from HLA-C*04:01:01:01 by one nucleotide in exon 3.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Alelos , Secuencia de Bases , NucleótidosRESUMEN
HLA-C*03:04:74 differs from HLA-C*03:04:01:01 by one nucleotide in exon 6.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Alelos , NucleótidosRESUMEN
HLA-B*35:563 differs from HLA-B*35:03:01:01 by one nucleotide in exon 4.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-B , Humanos , Alelos , Antígenos HLA-B/genética , NucleótidosRESUMEN
HLA-B*15:638 differs from HLA-B*15:01:01:01 by one nucleotide in exon 2.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-B , Humanos , Alelos , Antígenos HLA-B/genética , NucleótidosRESUMEN
HLA-DQB1*03:03:29 differs from HLA-DQB1*03:03:02:01 by one nucleotide in exon 2.
Asunto(s)
Cadenas beta de HLA-DQ , Humanos , Alelos , Secuencia de Bases , Pueblos del Este de Asia , Cadenas beta de HLA-DQ/genética , NucleótidosRESUMEN
HLA-C*15:02:58 differs from HLA-C*15:02:01:01 by one nucleotide in exon 1.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Alelos , Secuencia de Bases , NucleótidosRESUMEN
HLA-A*02:1075 differs from HLA-A*02:07:01:01 by one nucleotide in exon 5.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-A , Humanos , Alelos , Análisis de Secuencia de ADN , NucleótidosRESUMEN
HLA-C*07:1029 differs from HLA-C*07:02:01:01 by one nucleotide in exon 5.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Alelos , Secuencia de Bases , NucleótidosRESUMEN
HLA-B*35:568 differs from HLA-B*35:03:01:01 by one nucleotide in exon 4.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-B , Humanos , Alelos , Análisis de Secuencia de ADN , Antígenos HLA-B/genética , NucleótidosRESUMEN
HLA-A*02:07:22 differs from HLA-A*02:07:01:01 by one nucleotide in exon 3.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-A , Humanos , Alelos , Análisis de Secuencia de ADN , NucleótidosRESUMEN
HLA-DRB1*12:01:12 differs from HLA-DRB1*12:01:01:01 by one nucleotide in exon 2.
Asunto(s)
Pueblos del Este de Asia , Cadenas HLA-DRB1 , Humanos , Alelos , Secuencia de Bases , Cadenas HLA-DRB1/genética , NucleótidosRESUMEN
HLA-C*01:239 differs from HLA-C*01:02:01:01 by one nucleotide in exon 4.
Asunto(s)
Antígenos HLA-C , Humanos , Alelos , Pueblos del Este de Asia , Antígenos HLA-C/genética , Nucleótidos , Análisis de Secuencia de ADNRESUMEN
HLA-B*13:176 differs from HLA-B*13:02:01:01 by one nucleotide in exon 1.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-B , Antígeno HLA-B13 , Humanos , Alelos , Antígenos HLA-B/genética , Nucleótidos , Análisis de Secuencia de ADN , Antígeno HLA-B13/genéticaRESUMEN
HLA-C*04:01:152 differs from HLA-C*04:01:01:01 by one nucleotide in exon 1.
Asunto(s)
Pueblos del Este de Asia , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Alelos , Secuencia de Bases , Nucleótidos , Análisis de Secuencia de ADNRESUMEN
HLA-A*68:302 differs from HLA-A*68:01:02:01 by one nucleotide in exon 4.