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1.
Acta Radiol ; 61(9): 1228-1239, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-31986895

RESUMEN

BACKGROUND: Presurgical grading is particularly important for selecting the best therapeutic strategy for meningioma patients. PURPOSE: To investigate the value of histogram analysis of diffusion kurtosis imaging (DKI) maps in the differentiation of grades and histological subtypes of meningiomas. MATERIAL AND METHODS: A total of 172 patients with histopathologically proven meningiomas underwent preoperative magnetic resonance imaging (MRI) and were classified into low-grade and high-grade groups. Mean kurtosis (MK), fractional anisotropy (FA), and mean diffusivity (MD) histograms were generated based on solid components of the whole tumor. The following parameters of each histogram were obtained: 10th, 25th, 75th, and 90th percentiles, mean, median, maximum, minimum, and kurtosis, skewness, and variance. Comparisons of different grades and subtypes were made by Mann-Whitney U test, Kruskal-Wallis test, ROC curves analysis, and multiple logistic regression. Pearson correlation was used to evaluate correlations between histogram parameters and the Ki-67 labeling index. RESULTS: Significantly higher maximum, skewness, and variance of MD, mean, median, maximum, variance, 10th, 25th, 75th, and 90th percentiles of MK were found in high-grade than low-grade meningiomas (all P < 0.05). DKI histogram parameters differentiated 7/10 pairs of subtype pairs. The 90th percentile of MK yielded the highest AUC of 0.870 and was the only independent indicator for grading meningiomas. Various DKI histogram parameters were correlated with the Ki-67 labeling index (P < 0.05). CONCLUSION: The histogram analysis of DKI is useful for differentiating meningioma grades and subtypes. The 90th percentile of MK may serve as an optimal parameter for predicting the grade of meningiomas.


Asunto(s)
Imagen de Difusión Tensora/métodos , Neoplasias Meníngeas/diagnóstico por imagen , Meningioma/diagnóstico por imagen , Anciano , Anisotropía , Medios de Contraste , Femenino , Humanos , Masculino , Neoplasias Meníngeas/patología , Meningioma/patología , Persona de Mediana Edad , Clasificación del Tumor , Valor Predictivo de las Pruebas , Estudios Prospectivos
2.
Int J Mol Sci ; 20(8)2019 Apr 24.
Artículo en Inglés | MEDLINE | ID: mdl-31022906

RESUMEN

An effective and rapid way to detect thiophanate-methyl residue on mulberry fruit is important for providing consumers with quality and safe of mulberry fruit. Chemical methods are complex, time-consuming, and costly, and can result in sample contamination. Rapid detection of thiophanate-methyl residue on mulberry fruit was studied using laser-induced breakdown spectroscopy (LIBS) and hyperspectral imaging (HSI) techniques. Principal component analysis (PCA) and partial least square regression (PLSR) were used to qualitatively and quantitatively analyze the data obtained by using LIBS and HSI on mulberry fruit samples with different thiophanate-methyl residues. The competitive adaptive reweighted sampling algorithm was used to select optimal variables. The results of model calibration were compared. The best result was given by the PLSR model that used the optimal preprocessed LIBS-HSI variables, with a correlation coefficient of 0.921 for the prediction set. The results of this research confirmed the feasibility of using LIBS and HSI for the rapid detection of thiophanate-methyl residue on mulberry fruit.


Asunto(s)
Contaminación de Alimentos/análisis , Frutas/química , Morus/química , Plaguicidas/análisis , Tiofanato/análisis , Diseño de Equipo , Estudios de Factibilidad , Rayos Láser , Análisis de los Mínimos Cuadrados , Análisis de Componente Principal , Espectroscopía Infrarroja Corta/instrumentación , Espectroscopía Infrarroja Corta/métodos
3.
Sensors (Basel) ; 18(7)2018 Jun 28.
Artículo en Inglés | MEDLINE | ID: mdl-29958467

RESUMEN

Mulberry trees are an important crop for sericulture. Pests can affect the yield and quality of mulberry leaves. This study aims to develop a hyperspectral imaging system in visible and near-infrared (NIR) region (400⁻1700 nm) for the rapid identification of Diaphania pyloalis larvae and its damage. The extracted spectra of five region of interests (ROI), namely leaf vein, healthy mesophyll, slight damage, serious damage, and Diaphania pyloalis larva at 400⁻1000 nm (visible range) and 900⁻1700 nm (NIR range), were used to establish a partial least squares discriminant analysis (PLS-DA) and least-squares support vector machines (LS-SVM) models. Successive projections algorithm (SPA), uninformation variable elimination (UVE), UVE-SPA, and competitive adaptive reweighted sampling were used for variable selection. The best models in distinguishing between leaf vein, healthy mesophyll, slight damage and serious damage, leaf vein, healthy mesophyll, and larva, slight damage, serious damage, and larva were all the SPA-LS-SVM models, based on the NIR range data, and their correct rate of prediction (CRP) were all 100.00%. The best model for the identification of all five ROIs was the UVE-SPA-LS-SVM model, based on visible range data, which had the CRP value of 97.30%. In summary, visible and near infrared hyperspectral imaging could distinguish Diaphania pyloalis larvae and their damage from leaf vein and healthy mesophyll in a rapid and non-destructive way.


Asunto(s)
Larva/fisiología , Morus/parasitología , Mariposas Nocturnas/fisiología , Hojas de la Planta/parasitología , Espectroscopía Infrarroja Corta/métodos , Análisis Espectral/métodos , Animales , Análisis de los Mínimos Cuadrados , Modelos Teóricos , Máquina de Vectores de Soporte
4.
Phys Chem Chem Phys ; 18(15): 9918-21, 2016 Apr 21.
Artículo en Inglés | MEDLINE | ID: mdl-27032062

RESUMEN

We have examined the catalytic activity of four different iron-based oxides doped with foreign elements using three common driving forces. The data clearly demonstrate that their water oxidation catalytic activity differ widely under different driving forces.

5.
Phys Chem Chem Phys ; 17(16): 10648-55, 2015 Apr 28.
Artículo en Inglés | MEDLINE | ID: mdl-25804787

RESUMEN

CuFe2O4 nanocrystallines with cubic jacobsite structure have been obtained by heat treatment of the coprecipitation product, which were synthesized by the reaction of Cu(2+) ions and Fe(3+) ions under alkaline conditions. Reported here is the first copper-based catalyst for photocatalytic water oxidation using [Ru(bpy)3]Cl2 as the photosensitizer and Na2S2O8 as the sacrificial electron acceptor, respectively. An apparent TOF value of 1.2 µmol s(-1) m(-2) and an oxygen yield of 72.8% were obtained with CuFe2O4. The apparent TOF value with CuFe2O4 (1.2 µmol s(-1) m(-2)) is the highest value among all heterogeneous photocatalytic water oxidation systems. CuFe2O4 can be easily separated from reaction solution by magnetic separation while maintaining excellent water oxidation activity in the fourth and fifth runs. The surface conditions of CuFe2O4 are slightly absent after examination by X-ray photoelectron spectroscopy (XPS) before and after the photocatalytic reaction.

6.
Dalton Trans ; 52(6): 1797-1805, 2023 Feb 07.
Artículo en Inglés | MEDLINE | ID: mdl-36656043

RESUMEN

Urea splitting is a highly promising technology for hydrogen production to cope with the fossil energy crisis, which requires the development of catalysts with high electrocatalytic activity. In this article, Crx-FeCo2P/NF catalysts were synthesized by hydrothermal and low-temperature phosphorylation and used in the overall urea splitting process. Cr0.15-FeCo2P/NF and Cr0.1-FeCo2P/NF exhibited excellent urea oxidation reaction (UOR) activity (potential of 1.355 V at 100 mA cm-2) and hydrogen evolution reaction (HER) activity (overpotential of 173 mV at 10 mA cm-2) in 0.5 M urea solution containing 1 M KOH. In the assembled Cr0.15-FeCo2P/NF//Cr0.1-FeCo2P/NF electrolytic cell, only a small voltage of 1.50 V is needed to reach 10 mA cm-2. Density functional theory (DFT) calculation results demonstrate that an appropriate amount of Cr doping accelerates the kinetic performance of hydrogen production as well as improving the metallic properties of the electrode.

7.
Dalton Trans ; 52(3): 763-773, 2023 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-36594242

RESUMEN

Here, we synthesized a series of Ni3S2/MxSy-NiCo LDH materials (M = Cu, Fe, V, Ce, and Bi) by a two-step hydrothermal method for the first time, which display excellent oxygen evolution reaction (OER) and urea oxidation reaction (UOR) properties. M (M = Cu, Fe, V, Ce, and Bi) ions were firstly doped into NiCo LDH to change the original electronic structure and enhance the activity of the LDH. Then, Ni3S2 and MxSy were introduced by sulfurization of the Ni support and doping cations, and the combination of Ni3S2, MxSy and NiCo-LDH improved the electron transfer rate and activity of the original material. With Ni3S2/Bi2S3-NiCo LDH/NF as anode and Ni3S2/CuS-NiCo LDH as cathode, an electrolytic cell can reach 10 mA cm-2 at 1.622 V with outstanding durability for overall water splitting. In addition, with Ni3S2/Bi2S3-NiCo LDH/NF as both electrodes, it can reach 10 mA cm-2 at 1.56 V with outstanding durability for overall urea splitting, which is better than that of the overall water splitting. Density functional theory (DFT) calculation shows that the superior electrocatalytic activity can be explained by the water adsorption energy being optimized and enhanced conductivity. This study provides a new idea for improving the catalytic activity and stability of non-noble metals instead of noble metals.

8.
J Colloid Interface Sci ; 630(Pt A): 512-524, 2023 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-36270172

RESUMEN

Electrocatalytic water splitting has become one of the most promising and effective ways to solve energy crises and environmental pollution.In this work, a series of M-doped Co3O4 materials with foreign ions (M = Ce, Ni and Fe) were grown on a Ni foam substrate through classical hydrothermal and calcination methods.It is worth noting that not all doping of foreign ions can promote the electrocatalytic performance of intrinsic materials.The Ce-doped Co3O4 material shows excellent oxidation properties of water (overpotential of 320 mV at 50 mA cm-2) and urea (potential of 1.39 V at 50 mA cm-2).However, the doping of Ni reduces the water oxidation performance of the intrinsic material (overpotential of 410 mV@ 50 mA cm-2), and the doping of Fe reduces the urea oxidation performance of the inherent material (potential of 1.45 V@ 50 mA cm-2).A series of experimental results indicate that the improved activity of the Ce-Co3O4 electrode is attributed to faster electron transport capacity, higher exposure to active sites, and improved conductivity due to doping of Ce. It is noteworthy that the doping of these ions does alter the rate-determination step for the water oxidation reaction. The stability test demonstrated that the current density of water oxidation and urea oxidation of the catalyst had no significant attenuation after a long electrocatalytic activity test. This work provides a new idea for improving the electrocatalytic activity of catalysts by a doping strategy.

9.
Dalton Trans ; 52(25): 8811-8817, 2023 Jun 27.
Artículo en Inglés | MEDLINE | ID: mdl-37323005

RESUMEN

The development of efficient and stable non-precious-metal-based electrocatalysts is essential for practical water splitting applications. The electrolysis of water for hydrogen production is a green and efficient method, while urea electrolysis can improve energy conversion efficiency. In this paper, W-Ni3S2/NiS catalysts with heterogeneous structures were synthesized via a one-step hydrothermal method using a W-doping-induced phase transition strategy. The doping of W modulates the morphology of the catalyst, which can form uniform nanorod arrays and improve the activity of the electrocatalyst. In an alkaline solution of 1 M KOH and 0.5 M urea, W-Ni3S2/NiS requires a potential of only 1.309 V to achieve a current density of 10 mA cm-2. An electrolyzer containing urea with W-Ni3S2/NiS as both the cathode and anode can drive a current density of 10 mA cm-2 with a potential of only 1.569 V and has relatively good stability after testing for 20 h. Experimental results show that the improvement in the catalytic activity is due to the rapid charge transfer, exposure of more active sites and better conductivity. Density functional theory calculations show that the W-Ni3S2 material exhibits higher urea adsorption energy, indicating that urea is preferentially adsorbed on its surface. The NiS material shows more state density near the Fermi level, indicating that the introduction of this material enhances the conductivity of the W-Ni3S2/NiS material. The synergistic catalysis of the two materials promoted the improvement of the catalytic activity. This work provides new ideas for the development of highly efficient and stable catalysts by means of doping and interface construction.

10.
Dalton Trans ; 52(45): 16943-16950, 2023 Nov 21.
Artículo en Inglés | MEDLINE | ID: mdl-37929706

RESUMEN

The shortage of freshwater resources in the world today has limited the development of water splitting, and our eyes have turned to the abundant seawater. The development of relatively low-toxicity and high-efficiency catalysts is the most important area in seawater electrolysis. In this paper, the preparation of NiS2@Co4S3@FeS via a hydrothermal method on nickel foam has been studied for the first time. In the process of vulcanization, Fe will first generate FeS by virtue of its high affinity for vulcanization. Once Fe is vulcanized, the residual sulfur will be used to generate NiS2, while the vulcanization of Co requires a higher sulfur concentration and reaction temperature; thus, Co4S3 will be generated last. NiS2@Co4S3@FeS is confirmed to have excellent hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) catalytic properties in alkaline seawater. Its unique structure allows it to expose more reaction centres, and the synergies between the multiple metals optimize the charge distribution of the material and accelerate the OER and HER kinetics. NiS2@Co4S3@FeS requires overpotentials of only 122 mV and 68 mV for the OER and HER when reaching 10 mA cm-2, which is superior to most catalysts reported to date for seawater electrolysis, and the material displays acceptable stability. In an electrolytic cell composed of both positive and negative electrodes, when the current density is 10 mA cm-2, the NiS2@Co4S3@FeS material displays a low overpotential of only 357 mV for seawater splitting. Density functional theory shows that the FeS electrode has the optimum Gibbs free energy of H to accelerate reaction kinetics, and the synergistic catalysis of the NiS2, Co4S3 and FeS materials promotes the hydrogen production activity of the NiS2@Co4S3@FeS electrode. This work proposes a novel idea for designing environmentally friendly seawater splitting catalysts.

11.
Dalton Trans ; 52(30): 10499-10506, 2023 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-37448260

RESUMEN

Water electrolysis is relatively an environmentally friendly hydrogen production technology, but due to the slow transfer of four electrons in the anodic oxidation reaction, it needs a theoretical voltage of up to 1.23 V. Therefore, in this experiment, a series of transition metal oxides, ACo2O4 (A = Fe, Cu, Zn, Ni), was synthesized on Ni foam current collectors by a hydrothermal and calcination method, and the material was applied in urea electrolysis to produce hydrogen. What is noteworthy is that the CuCo2O4 electrode has a unique flower-like nanoneedle structure, and has a larger electrochemical active area, more reactive active sites, and a faster charge transfer rate. In 1.0 M KOH and 0.5 M urea solution, CuCo2O4 provides a potential of only 1.268 V at a current density of 10 mA cm-2 during the urea oxidation reaction (UOR), while in 1.0 M KOH solution, with the same current density, the oxygen evolution reaction (OER) is required to provide a potential of 1.53 V, indicating that the UOR can effectively replace the OER. Density functional theory calculations show that the CuCo2O4 material exhibits Gibbs free energy of the hydrogen closest to zero, thus promoting the electrochemistry performance of the electrode. In a cell composed of CuCo2O4//CuCo2O4, the current density of 10 mA cm-2 can be achieved by providing a potential of only 1.509 V. This work offers a novel scheme for reducing energy consumption of the OER and improving catalytic performance of the UOR.

12.
Dalton Trans ; 52(37): 13161-13168, 2023 Sep 26.
Artículo en Inglés | MEDLINE | ID: mdl-37656128

RESUMEN

Urea electrolysis can be used to treat wastewater containing urea and alleviate the energy crisis, so it is one of the best ways to solve environmental and energy problems. This paper reports the synthesis of M doped NiVS (M = Co, Ce and Cr) composites by a simple hydrothermal process for the first time. What is noteworthy is that the Ce-NiVS material as a catalytic electrode requires only 141 mV overpotential for the hydrogen evolution reaction (HER) and 1.291 V potential for the urea oxidation reaction (UOR) at a current density of 10 mA cm-2 in 1.0 M KOH and 0.5 M urea mixed alkaline solution. Using Ce-NiVS/NF as both the anode and cathode for urea electrolysis, a current density of 10 mA cm-2 is driven by a voltage of only 1.55 V, which is better than most previous catalysts. Experimental results demonstrate that the excellent catalytic activity of Ce-NiVS materials is due to the formation of a large number of active sites and the improvement of conductivity due to doping with Ce. Density functional theory calculation shows that the VS4 material has a small Gibbs free energy of hydrogen adsorption, which plays a major role in the hydrogen production process, and Ce-NiS has a higher density of states (DOS) near the Fermi level, indicating that Ce-NiS has better electronic conductivity. The synergistic catalysis of VS4 and Ce-NiS promoted the hydrogen production performance of the Ce-NiVS material. This work provides guidance for the optimization and design of low-cost electrocatalysts to replace expensive precious metal-based electrocatalysts for overall urea electrolysis.

13.
Dalton Trans ; 52(18): 6052-6060, 2023 May 09.
Artículo en Inglés | MEDLINE | ID: mdl-37057411

RESUMEN

Electrochemical water splitting as an important means of obtaining high purity hydrogen fuel has attracted great interest. In this study, the structural engineering of complex WNiM-WNi LDH (M = Se, S, or P) was firstly developed by in situ growth on Ni foam for use in overall water splitting and the urea oxidation reaction. These WNiM-WNi LDH (M = Se, S, or P) catalysts exhibit outstanding electrocatalytic performance in the hydrogen evolution reaction (HER), oxygen evolution reaction (OER) and urea oxidation reaction (UOR), respectively. An overpotential of only 64 mV of OER is required for WNiS-WNi LDH and 126 mV of HER is required for WNiP-WNi LDH to achieve 10 mA cm-2. The WNiSe-WNi LDH materials display a particularly outstanding performance for UOR, requiring a potential of 1.25 V to drive 10 mA cm-2. Moreover, the optimized WNiS-WNi LDH as an anode and WNiP-WNi LDH as a cathode can achieve 10 mA cm-2 at a low cell voltage of 1.45 V in 1 M KOH solution for overall water splitting. The density functional theory calculations show that the introduction of the NiP2 and WP material greatly reduces the Gibbs free energy of the hydrogen adsorption of the material.

14.
Dalton Trans ; 51(21): 8240-8248, 2022 May 31.
Artículo en Inglés | MEDLINE | ID: mdl-35578808

RESUMEN

Atomic doping and surface engineering are regarded as a promising method for improving their electrochemistry performance toward the water oxidation reaction and urea oxidation reaction (UOR) for layered double hydroxides (LDHs) containing low cost transition metals. However, the mechanism of how foreign ion doping and interface construction enhance catalytic activity remains unclear. In this work, NiFe LDH is reported as an example where Ce (Ce-NiFe LDH) is doped or the interface is constructed with Ce(OH)3(Ce(OH)3@NiFe LDH), and water oxidation and urea oxidation reaction are used as probe reactions. The Ce(OH)3@NiFe LDH material shows superior electrocatalytic performance for the water oxidation reaction (at an overpotential of 220 mV@10 mA cm-2) and urea oxidation reaction (at a potential of 1.40 V@10 mA cm-2), which is one of the best electrocatalytic performances reported so far. After a long time of stability testing, it was found that the catalytic current had significant attenuation, and further characterization showed that the surface of the electrode would be oxidized to oxyhydroxide, which is the true active species. The experimental results demonstrate that foreign Ce and Fe atom doping and interface construction improve the exposure of active centres, enhance the electron transfer rate and reduce the impedance of the NiFe LDH material. It is worth noting that this work provides new ideas for designing efficient, stable and environmentally friendly catalysts for water splitting and urea oxidation by means of doping and interface construction.

15.
Dalton Trans ; 51(35): 13255-13262, 2022 Sep 13.
Artículo en Inglés | MEDLINE | ID: mdl-35979877

RESUMEN

Urea electrolysis is of great interest for energy-related applications, but it is limited by a complex six-electron transfer process with slow kinetics. Herein, the in situ growth of Cr-doped Co2P homogeneous nanoneedle arrays on nickel foam substrates (Cr-Co2P/NF) was reported for the first time by a typical hydrothermal and low-temperature phosphorization process. The appropriate amount of Cr doping was found to promote the electronic modulation of active centers and the expansion of the specific active surface area, resulting in the superior performance of the urea oxidation reaction (UOR). It is noteworthy that Cr0.4-Co2P/NF exhibited a superior performance of the UOR at an onset potential of 1.290 V and a cell voltage of 1.333 V at 50 mA cm-2 in 1 M KOH containing 0.5 M urea, which is one of the best catalytic activities reported so far. The experimental results demonstrate that the enhanced catalytic activity can be attributed to favorable electronic regulation, an improved charge transfer rate and increased exposure to active sites. Density functional theory (DFT) calculation indicates that the appropriate doping of Cr effectively regulates and controls the adsorption energy of urea and the conductivity of the Co2P material itself. This work provides new ideas for the development of robust catalysts for the electrolysis of urea through doping strategies.

16.
Dalton Trans ; 51(6): 2444-2451, 2022 Feb 08.
Artículo en Inglés | MEDLINE | ID: mdl-35048936

RESUMEN

At present, global resources are nearly exhausted and environmental pollution is becoming more and more serious, so it is urgent to develop efficient catalysts for hydrogen production. Herein, nitrogen and iron co-doped Ni3S2 and NiP2 heterostructures with high efficiency oxygen evolution reaction (OER) and urea oxidation reaction (UOR) performances were firstly successfully prepared on nickel foam by hydrothermal and high-temperature calcination methods. Benefiting from the hierarchical structure, the exposure of more active sites and the doping effect of N and Fe, the N-Fe-Ni3S2@NiP2/NF material showed excellent electrocatalytic activity for the OER and UOR. The N-Fe-Ni3S2@NiP2/NF material displays excellent catalytic OER performance; the overpotential is only 251 mV to drive 100 mA cm-2 current density, while for the UOR, the potential is only 1.353 V to drive 100 mA cm-2 current density, which is one of the best catalytic activities reported so far. It is worth noting that scanning electron microscopy showed that the surface of N-Fe-Ni3S2@NiP2/NF is rough and has some mesopores, which may have resulted in an increase of active sites during the electrocatalytic process. The N-Fe-Ni3S2@NiP2/NF electrode couple also has relatively long-term durability in alkaline solutions, maintaining a stable current density for 15 h at 1.35 V. The density functional theory (DFT) calculation shows that the in situ generated Fe doped nanooxides exhibit strong water adsorption energy, which may be one of the reasons for the good catalytic activity. Our work is conducive to the rational design of electrocatalysts for efficient hydrogen production from water splitting and wastewater treatment.

17.
Dalton Trans ; 51(4): 1594-1602, 2022 Jan 25.
Artículo en Inglés | MEDLINE | ID: mdl-34994364

RESUMEN

Transition metal materials with high efficiency and durable electrocatalytic water splitting activity have attracted widespread attention among scientists. In this work, two cation co-doped Ni3S4 nanoarrays grown on a Ni foam support were firstly synthesized through a typical two step hydrothermal process. Cu and Fe co-doping can regulate the internal electron configuration of the material, thus reducing the activation energy of the active species. Moreover, density functional theory calculations demonstrate that a low Ni2+ amount improves the adsorption energy of H2O, which facilitates the formation and reaction of intermediate species in the water splitting process. The experimental results indicate that the Cu and Fe co-doped Ni3S4 material has superior electrochemical activity for water oxidation reaction to pure Ni3S4, Fe doped Ni3S4 and Cu doped Ni3S4. The Fe-Cu-Ni3S4 material displays a significantly enhanced electrocatalytic performance with low overpotentials of 230 mV at 50 mA cm-2 and 260 mV at 100 mA cm-2 for the oxygen evolution reaction under alkaline conditions. It's worth noting that when Fe-Cu-Ni3S4 was used as the anode and cathode, a small cell voltage of 1.59 V at 10 mA cm-2 was obtained to achieve stable overall water splitting. Our work will afford a novel view and guidance for the preparation and application of efficient and environmentally friendly water splitting catalysts.

18.
ISA Trans ; 129(Pt B): 309-320, 2022 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-35305817

RESUMEN

Infrared thermal technology plays a vital role in the health condition monitoring of gearbox. In the traditional infrared thermal technology-based methods, Gaussian pyramid is applied as the feature extraction approach, which has disadvantages of noise influence and information missing. Focus on such disadvantages, an improved multi-scale decomposition method combined with convolutional neural network is proposed to extract the fault features of the multi-scale infrared images in this paper. It can enlarge the data length at large scales, and thus reduce the fluctuations of feature values and reserve the fault information. The effectiveness of the proposed method is validated using the experiment infrared data of one industrial gearbox. Results demonstrate that our proposed method has the best performance comparing with five methods.

19.
Methods Mol Biol ; 2475: 79-96, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35451750

RESUMEN

Vascular endothelial growth factor (VEGF) plays a vital role in angiogenesis, and is also involved in tumor cell growth and immunosuppression, showing very complex roles. VEGF-exosomes are released by tumor endothelial cells (ECs) following anti-angiogenesis therapies (AATs). Transwell assays enable the detection of migration and invasion capacities of tumor cells. Matrigel assays are used to evaluate the angiogenesis capacities of ECs. Here we describe the detection of VEGF content in exosomes by nano-flow cytometry, enzyme-linked immunosorbent assay (ELISA), and western blotting, and demonstrate the procedure for detection of the colon formation of tumor cells induced by exosomes, the angiogenesis of tumor cells co-cultured with ECs, the angiogenesis of tumor cells induced by exosomes in Matrigel assay in vitro and tumor xenografts.


Asunto(s)
Exosomas , Neoplasias , Animales , Movimiento Celular , Proliferación Celular , Células Endoteliales/metabolismo , Exosomas/metabolismo , Humanos , Neoplasias/metabolismo , Neovascularización Patológica/metabolismo , Factor A de Crecimiento Endotelial Vascular/metabolismo
20.
Front Surg ; 9: 1089930, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36684273

RESUMEN

Background: Neoadjuvant therapy following minimally invasive esophagectomy is recommended as the standard treatment for locally advanced esophageal squamous carcinoma cells (ESCC). Postoperative atrial fibrillation (POAF) after esophagectomy is common. We aimed to determine the risk factors and construct a nomogram model to predict the incidence of POAF among patients receiving neoadjuvant therapy. Methods: We retrospectively included patients with ESCC receiving neoadjuvant chemotherapy (nCT), neoadjuvant chemoradiotherapy (nCRT), or neoadjuvant immunochemotherapy (nICT) following minimally invasive esophagectomy (MIE) for analysis. Patients without a history of AF who did not have any AF before surgery and who developed new AF after surgery, were defined as having POAF. We applied a LASSO regression analysis to avoid the collinearity of variables and screen the risk factors. We then applied a multivariate regression analysis to select independent risk factors and constructed a nomogram model to predict POAF. We used the receiver operating characteristic (ROC) curve, calibration curve, and decision curve analysis (DCA) curve to evaluate the nomogram model. Results: A total of 202 patients were included for analysis, with 35 patients receiving nCRT, 88 patients receiving nCT, and 79 patients receiving nICT. POAF occurred in 34 (16.83%) patients. There was no significant difference in the distribution of neoadjuvant types between the POAF group and the no POAF group. There was a significant increase in postoperative hospital stay (p = 0.04), hospital expenses (p = 0.01), and comprehensive complication index (p < 0.001). The LASSO analysis screened the following as risk factors: blood loss; ejection fraction (EF); forced expiratory volume in 1 s; preoperative albumin (Alb); postoperative hemoglobin (Hb); preoperative Hb; hypertension; time to surgery; age; and left atrial (LA) diameter. Further, preoperative Alb ≤41.2 g/L (p < 0.001), preoperative Hb >149 g/L (p = 0.01), EF >67.61% (p = 0.008), and LA diameter >32.9 mm (p = 0.03) were determined as independent risk factors of POAF in the multivariate logistic analysis. The nomogram had an area under the curve (AUC) of 0.77. The Briser score of the calibration curve was 0.12. The DCA confirmed good clinical value. Conclusions: Preoperative Alb ≤41.2 g/L, LA diameter >32.9 mm, preoperative Hb >149 g/L, and EF >67.61% were determined as the risk factors for POAF among patients with ESCC. A novel and valuable nomogram was constructed and validated to help clinicians evaluate the risk of POAF and take personalized treatment plans.

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