Phytotoxic effect of the insecticide imidacloprid in Phaseolus vulgaris L. plant and evaluation of its bioaccumulation and translocation by electrochemical methods.

The objective of this work is to study the toxicological effect of the imidacloprid (IMD) on common bean plants (Phaseolus vulgaris L) when used at high doses and its quantification by electrochemical method. Common bean plants were exposed to increasing concentrations of IMD and the different plant tissues were subjected to various analyses. The IMD detection in different tissues of the bean plant was performed after extraction on the metallic silver electrode using square wave voltammetry. The analytical and calibration parameters (Slope, correlation coefficient, linear range, detection limit and relative standard deviation) were calculated for the different plant tissues. The effect of different doses (5.0 × 10-3 to 5.0 × 10-2 mol L-1) of IMD was evaluated on germination, seedling (vigour, growth) and photosynthetic pigments in the bean plant. The results indicate that germination rate and seed vigour index reduced significantly (p ≤ 0.05) only in the applied concentrations above the recommended dose. A similar effect of IMD was observed on seedling development in term of roots length, plant length, number of leaves and number of nods. Concerning pigments content, chlorophyll a, b and total chlorophyll maximally decreased by 95.26%, 80.44% and 82.15% respectively at high applied dose. The bioaccumulation and translocation behaviour of IMD in bean plant was investigated, revealing that the IMD can be bioaccumulated in roots and can easily be translocated into stems and leaves.

Electrochemical detection of ornidazole in commercial milk and water samples using an electrode based on green synthesis of silver nanoparticles using cellulose separated from Phoenix dactylifera seed.

The widespread use of antibiotics has contributed to the control of disease and the nutritional well-being of livestock. Antibiotics reach the environment via excretions (urine and feces) from human and domestic animals, through non proper disposal or handling of unused drugs. The present study describes a green method for the synthesis of silver nanoparticle (AgNPs) using cellulose extracted from Phoenix dactylifera seed powder via mechanical stirrer method for the electroanalytical determination of ornidazole (ODZ) in milk and water samples. The cellulose extract is used as the reducing and stabilizer agent for the synthesis of AgNPs. The obtained AgNPs were characterized by UV-Vis, SEM and EDX, presenting a spherical shape and an average size of 48.6 nm. The electrochemical sensor (AgNPs/CPE) was fabricated by dipping a carbon paste electrode (CPE) in the AgNPs colloidal solution. The sensor shows acceptable linearity with ODZ concentration in the linear range from 1.0 × 10-5 to 1.0 × 10-3 M with a limit of detection (LOD =3S/P) and quantification (LOQ =10S/P) of 7.58 × 10-7 M and 2.08 × 10-6 M respectively.

Recent trends on electrochemical determination of antibiotic Ciprofloxacin in biological fluids, pharmaceutical formulations, environmental resources and foodstuffs: Direct and indirect approaches.

In the last few decades, pharmaceuticals, credited with saving millions of lives, have emerged as a new class of environmental contaminants. These compounds can have both chronic and acute harmful effects on aquatic ecosystems and consequently on human health. Therefore, there is an urgent need for the development of extremely sensitive, portable, and low-cost devices to perform analysis. In the present review article, recent reports on the application of various voltammetric and photo-electrochemical techniques using different electrode materials for the determination of antibiotic Ciprofloxacin (CIPRO) are reported. This review provides an insight into direct and indirect electrochemical approaches as well as the photoelectrochemical methods used for the determination of CIPRO. Emphasis is put on the applications of unmodified and modified carbon-based electrodes considering the modifier, supporting electrolytes, analytical method, concentration range, limit of detection, and real matrices. Carbon-based electrodes are the most used materials attributed to their commercial availability, reduced cost, high chemical stability, and non-toxicity.