Acquired dysfibrinogenemia caused by monoclonal production of immunoglobulin lambda light chain.

Disorders of fibrinogen are usually caused by genetic mutations that result in low protein levels (hypofibrinogenemia) or an abnormal molecule (dysfibrinogenemia). However, environmental and plasma factors can have an acquired effect on its expression or function. For example, antibodies can bind fibrinogen and/or fibrin to interfere with polymerization and inhibit coagulation. The objective here was to determine the cause of dysfibrinogenemia in a 63-year-old man. Despite a low functional fibrinogen concentration and prolonged thrombin time, no inherited fibrinogen abnormality could be detected after extensive protein analysis and gene sequencing. Thus, electrophoresis methods and fibrinogen binding studies were used to establish the cause of the acquired dysfibrinogenemia. An immunoglobulin lambda light chain was found to bind fibrinogen as a monomer. It had no significant effect on fibrinopeptide release, but caused substantial defects in all other stages of thrombin-catalyzed fibrin polymerization. Binding to fibrinogen also seemed to prevent the light chain from being filtered through the kidneys, causing only low levels of it in the urine. Once in the urine, the lambda chain lost its anti-fibrinogen activity, apparently due to dimerization. The 63-year-old patient acquired dysfibrinogenemia from a monoclonal production of lambda light chain that bound and inhibited the function of fibrinogen. At age 64.5 he was diagnosed with monoclonal gammopathy of undetermined significance, explaining the abnormal immunoglobulin chain production. This case was particularly unusual in that the inhibition of fibrin polymerization was caused by a single immunoglobulin light chain, rather than by a whole antibody molecule.

High-dose intravenous human immunoglobulin in chronic inflammatory demyelinating polyneuropathy.

We treated nine consecutive patients with chronic inflammatory demyelinating polyneuropathy (CIDP) with high-dose intravenous human immunoglobulin (HIG), and clinical recovery rapidly followed. Disability that had persisted for months or years was often reversed in days. There were no major adverse reactions to HIG infusions.

Blood lead levels in Dunedin 11 year old children.

Blood lead levels were determined for 579 eleven year old children. The range of blood lead levels was from 0.19 to 2.41 mumol/l with a geometric mean of 0.49 mumol/l (geometric SD 0.07) and an arithmetic mean of 0.54 mumol/l (arithmetic SD 0.24). Two children had levels above 1.45 mumol/l. There was no significant correlation between blood lead levels and socio-economic status. Ten children with elevated blood lead levels (greater than 1.11 mumol/l) were reassessed and the results from all but one child had returned to a lower level. In nine out of ten of these cases recent inside paint stripping activities had been carried out in the child's home.

Non-steroidal anti-inflammatory drugs and platelet function.

The effect of some newer non steroidal inflammatory drugs on platelet function has been assessed using platelet malondialdehyde production as a measure of cyclooxygenase activity. After single doses of the short acting NSAID's diflunisal, naproxen and sulindac, platelet malondialdehyde production was substantially diminished at one and three hours. It had largely recovered by 24 hours and had returned to normal by 48 hours. After aspirin malondialdehyde production was markedly reduced and was still down at 72 hours. After the long acting NSAID piroxicam, in doses providing blood levels comparable with those in long term treatment, malondialdehyde production was still down at 72 hours.

Cytomegalovirus infection and blood transfusion.

The incidence of cytomegalovirus antibody positivity has been determined in populations of blood donors in centres in the North and South Islands of New Zealand. No difference in incidence was found. Rates of antibody detection increased from approximately 30% in younger donors to approximately 65% in donors between the ages of 56 and 65 years. No sex difference in incidence was observed. The implications for policies governing the supply of cytomegalovirus antibody-free blood for transfusion are discussed.

Post-infectious encephalopathy simulating functional psychosis.

Three cases of post-infectious encephalopathy are presented in which behaviour changes occurred in the absence of neurological abnormality during convalescence from an influenza-like illness. The symptoms included aggresive behaviour, sleep reversal, sexual disinbition and a catatonic schizophrenia-like state. The prognosis was good.

Breast feeding and some reasons for electing to wean the infant: a report from the Dunedin Multidisciplinary Child Development Study.

Data are presented on the prevalence of breast feeding among 1037 Dunedin mothers who gave birth to infants between April 1972 and March 1973 and their reasons for weaning. Although breast feeding was becoming more common at that time, more than two-thirds of the mothers weaned their babies as a result of the difficulties they encountered. One of the most significant factors in lactation failure is the mismanagement of breast feeding by health professionals.

Dietary iron intakes and biochemical iron status of 15-49 year old women in New Zealand: is there a cause for concern?

AIM: To assess dietary iron intakes and biochemical iron status of a nationally representative sample of nonpregnant 15-49 year old women (n=1,751) in New Zealand. METHODS: A cross-sectional national survey was conducted in 1996/97. Women were selected via a multistage stratified cluster sampling procedure with increased sampling of Maori and Pacific women. Dietary iron intakes were estimated using a 24-hour diet recall. Biochemical iron status was assessed on a non-fasting venipuncture blood sample (n=1,047) via haemoglobin, mean cell volume, erythrocyte zinc protoporphyrin, transferrin receptors and serum ferritin. RESULTS: Average daily dietary iron intakes ranged from 9.6 mg/day among Pacific women to 10.5 mg/day among Maori women; 41% of 20-49 year olds and 45% of adolescents were at risk of low dietary iron intakes. The estimated percentage of 15-49 year old women with iron deficiency anaemia ranged from 1.4-5.5%, and for iron deficiency without anaemia from 0.7-12.6% depending on the age group and criteria used. CONCLUSIONS: The overall estimated prevalence of suboptimal biochemical iron status among 15-49 year old women in New Zealand ranged from 7-13%, which compared favourably with premenopausal women living in other western countries. This situation is, however, a public health concern given the potential negative functional consequences associated with even mild iron deficiency.

Molecular analysis of HLA-H gene mutations in New Zealand patients with haemochromatosis.

AIM: To determine the frequency of HLA-H gene mutations in New Zealand patients with haemochromatosis. METHODS: The Cys282Tyr and His63Asp mutations in the HLA-H gene were analyzed by polymerase chain reaction, restriction enzyme digestion and electrophoresis in two separate patient groups. The first was a group of 20 Christchurch patients with a definite clinical diagnosis of haemochromatosis. The second group consisted of 33 patients, with a provisional diagnosis of haemochromatosis, attending Dunedin Hospital for therapeutic venesection. RESULTS: All 20 Christchurch patients and 25 of the 33 (76%) Dunedin patients were homozygous for the Cys282Tyr mutation. After review of the clinical data, histology and response to venesection a diagnosis of haemochromatosis could be confidently excluded in six of the remaining eight patients. Despite atypical features, a diagnosis of haemochromatosis could not be excluded in the final two patients, one of whom was a compound heterozygote for the two mutations. CONCLUSIONS: Homozygosity for the Cys282Tyr mutation is closely associated with haemochromatosis in New Zealand patients. Molecular analysis of the HLA-H gene is indicated in the assessment of patients with iron overload including those currently being treated by venesection.

The performance of multiple joint procedures at one operation in a patient with haemophilia.

A haemophiliac patient with crippling degenerative joint disease is described in whom arthrodeses of the left knee and right ankle and total replacement of the left ankle were performed in the course of one operation. The reasons for this and for using a total ankle replacement are discussed.

Blood lead, intelligence, reading attainment, and behaviour in eleven year old children in Dunedin, New Zealand.

A study of blood lead levels and intelligence, reading, and behaviour problems was carried out using a sample of 579 Dunedin 11-yr-old children. The results suggested that when account was taken of social, environmental, and background factors, raised blood lead is associated with a small but statistically significant increase in children's general behaviour problems as reported by both parents and teachers. These results applied especially to the more specific problems of inattention and hyperactivity.

Fibrinogen Otago: a major alpha chain truncation associated with severe hypofibrinogenaemia and recurrent miscarriage.

A woman with a preliminary diagnosis of afibrinogenaemia was later found to have a functional fibrinogen of 0.06 mg/ml and markedly prolonged thrombin and reptilase times. The stoichiometry of fibrinopeptide release was normal but there was a gross delay in the polymerization of purified fibrin. Plasma protein electrophoresis showed an absence of normal fibrinogen and a novel anodal component which was confirmed as fibrinogen by immunofixation. Western blots of non-reducing SDS-PAGE gels indicated a molecular weight of 270 kD, compared to 340 kD for normal fibrinogen and similar analysis of reducing gels showed that the expected 67 kD A alpha chain was missing and replaced by a 30 kD band. This aberrant chain was not detected by the monoclonal antibody F-103, which recognizes the epitope formed by residues 259-276 of the A alpha chain. Cycle sequencing of the DNA encoding the F-103 epitope revealed the homozygous insertion of cytosine at position 4133 of the gene sequence. Predictably this translates as three new amino acids (268Gln-Glu-Pro) before termination at a new (TAG) stop codon. No abnormal A alpha chains could be detected in plasma from the woman's heterozygous son. The hypofibrinogenaemia observed is likely to be the result of diminished assembly and/or secretion of the truncated A alpha chains rather than enhanced extracellular degradation.

Hypofibrinogenemia in an individual with 2 coding (gamma82 A-->G and Bbeta235 P-->L) and 2 noncoding mutations.

We investigated the molecular basis of hypofibrinogenemia in a man with a normal thrombin clotting time. Protein analysis indicated equal plasma expression of 2 different Bbeta alleles, and DNA sequencing confirmed heterozygosity for a new Bbeta235 P-->L mutation. Protein analysis also revealed a novel gamma(D) chain, present at a ratio of 1:2 relative to the gamma(A) chain. Mass spectrometry indicated a 14 d decrease in the gamma(D)-chain mass, and DNA sequencing showed this was caused by a novel gamma82 A-->G substitution. DNA sequencing established heterozygosity for 2 further mutations: T-->C in intron 4 of the Aalpha gene and A-->C in the 3' noncoding region of the Bbeta gene. Studies on the man's daughter, together with plasma expression levels, discounted both the Aalpha and Bbeta mutations as the cause of the low fibrinogen, suggesting that the gamma82 mutation caused the hypofibrinogenemia. This was supported by analysis of 31 normal controls in whom the Bbeta mutations were found at polymorphic levels, with an allelic frequency of 5% for the Bbeta235 mutation and 42% for the Bbeta 3' untranslated mutation. The gamma82 mutation was, however, unique to the propositus. Residue gamma82 is located in the triple helix that separates the E and D domains, and aberrant packing of the helices may explain the decreased fibrinogen concentration. (Blood. 2000;95:1709-1713)

Soluble HLA antigens not detected in human immunoglobulin preparations.

Immunoglobulin concentrates prepared from subjects of known HLA specificity were assayed for the presence of soluble HLA antigens by the inhibition of cytotoxic HLA alloantisera using a microabsorption procedure. The immunoglobulin preparations inhibited alloantisera of all specificities tested, regardless of the HLA type of the immunoglobulin donor, indicating that the observed inhibition was non-specific. Addition of extra complement reduced the inhibitory effects of the immunoglobulin concentrates. It is concluded that the inhibition of HLA alloantisera demonstrated by this technique is due to the anticomplementary activity of the immunoglobulin preparations, rather than their content of specific soluble HLA antigens.