RESUMEN
The gastrointestinal (GI) tract plays a central role in the absorption, distribution, metabolism, and excretion of flavonoids, which ultimately define the health effects of these bioactives. These aspects are modulated by the interactions of flavonoids with other dietary components, environmental factors, the host, and the GI microbiota. Flavonoid can target molecules in the luminal content, the different GI tract cell types, and the microbiota. Importantly, flavonoid actions at the GI tract can have an impact systemically, e.g. on glucose homeostasis, lipid and energy metabolism, or cardiovascular risk factors. The beneficial actions of flavonoids at the GI include their capacity to: i) protect the intestinal epithelium against pharmacological insults and food toxins; ii) modulate the activity of enzymes involved in lipid and carbohydrate absorption; iii) maintain the intestinal barrier integrity; iv) modulate the secretion of gut hormones; v) modulate the GI tract immune system; vi) exert potential anti-colorectal cancer activity; and vii) shape microbiota composition and function. Further understanding of the mechanisms mediating the effects of flavonoids on the intestine (and its microbiota) is of critical importance given the relevance of the GI tract on sustaining overall health and of the widespread recommendations of increasing the intake of plant bioactives.
Asunto(s)
Flavonoides/metabolismo , Tracto Gastrointestinal/metabolismo , Enfermedades Gastrointestinales/metabolismo , Enfermedades Gastrointestinales/microbiología , Enfermedades Gastrointestinales/patología , Microbioma Gastrointestinal , Tracto Gastrointestinal/microbiología , Tracto Gastrointestinal/patología , Salud , HumanosRESUMEN
The content of [alpha]-tocopherol ([alpha]T) in isolated soybean (Glycine max, var Hood) embryonic axes was measured upon germination. Dry, high-vigor axes contained 1.2 [plus or minus] 0.1, nmol/axis and after an increase during the initial 6 h of imbibition, there was a decline to 1.0 [plus or minus] 0.1 nmol/axis at 24 h of incubation. Incubation in the presence of the redox-cycling agent paraquat (4 mM) for 24 h increased the [alpha]T content to 1.9 [plus or minus] 0.2 nmol/axis. When the incubation medium was supplemented with 500 [mu]M Fe-EDTA over 24 h, the content of [alpha]T increased to 1.8 [plus or minus] 0.1 nmol/axis. Isolated axes from soybean seeds stored for 56 months contained 6.5 [plus or minus] 0.3 nmol of [alpha]T/axis after 24 h of imbibition as compared to 1.0 [plus or minus] 0.1 nmol of [alpha]T/axis in axes from soybean seeds stored for 8 months. In all of these experimental situations, oxidant production as assessed in vivo by a fluorometric assay was increased by 4 mM paraquat (8-fold), 500 [mu]M iron (2-fold), and 56 months of storage (4-fold) after 24 h of imbibition. The data presented here suggest that the cellular content of [alpha]T is physiologically adjusted as a response to conditions of oxidative stress.
RESUMEN
Angiotensin II can induce oxidant stress by stimulating vascular superoxide production. Hypertension promotes mitochondrial function decline in brain, liver and heart. The aim of this study was to investigate whether a) hypertension is associated to kidney mitochondrial dysfunction, and b) angiotensin II blockade can reverse potential mitochondrial changes in hypertension. Four-month-old male spontaneously hypertensive rats (SHR) received drinking water containing candesartan (7.5 mg/kg/day, SHR+Cand), or no additions (SHR) for 4-months. Eight-month-old Wistar-Kyoto rats (WKY), that received water with no additions, were used as control. Systolic blood pressure, proteinuria, cortical glomerular area, and glomerular and tubulointerstitial alpha-smooth muscle actin labeling, were significantly higher, and creatinine clearance was significantly lower, in SHR relative to WKY and SHR+Cand. In SHR, kidney mitochondria membrane potential, and nitric oxide synthase and cytochrome oxidase activities were significantly lower than in WKY and SHR+Cand. In SHR, mitochondrial hydrogen peroxide production was significantly higher than in WKY and SHR+Cand. The results suggest that, in hypertension, increased mitochondrial oxidant production may mediate kidney mitochondria dysfunction. Candesartan preserved mitochondrial function, probably favoring the maintenance of adequate cellular and tissue function in the kidney. The known renal protective effects of candesartan in hypertension may be related to the improvement of mitochondrial function. This may be an additional or alternative explanation for some of the beneficial effects of AT1 receptor antagonists.
Asunto(s)
Bloqueadores del Receptor Tipo 1 de Angiotensina II/farmacología , Bencimidazoles/farmacología , Hipertensión/fisiopatología , Mitocondrias/efectos de los fármacos , Mitocondrias/fisiología , Tetrazoles/farmacología , Actinas/análisis , Angiotensina II/fisiología , Animales , Compuestos de Bifenilo , Presión Sanguínea/efectos de los fármacos , Creatinina/orina , Complejo IV de Transporte de Electrones/análisis , Peróxido de Hidrógeno/análisis , Inmunohistoquímica , Corteza Renal/efectos de los fármacos , Corteza Renal/patología , Corteza Renal/fisiopatología , Masculino , Potenciales de la Membrana/efectos de los fármacos , Mitocondrias/química , Óxido Nítrico Sintasa/análisis , Estrés Oxidativo/fisiología , Ratas , Ratas Endogámicas SHR , Ratas Endogámicas WKYRESUMEN
Based on the recognized capacity of (+)-catechin (CTCH) to prevent free radical-mediated damage in different biological systems, its role in the protection of human plasma from oxidation was investigated. Samples of human blood plasma were incubated with 50 mM AAPH [2,2'-azobis-(2-amidinopropane) clorhidrate] or AMVN [2,2'-azobis(2,4-valeronitrile)], in the absence or the presence of CTCH (0.01 to 1 mM). Lipid oxidation was evaluated measuring the formation of 2-thiobarbituric acid reactive substances (TBARS). Alpha-tocopherol (AT), beta-carotene (BC), and CTCH were measured by reverse phase HPLC with electrochemical detection. TBARS formation was dependent on incubation time and on the nature of the azocompound, yielding 4.8 +/- 0.9, and 14.9 +/- 3.4 microM MDA, after 4 h, in AAPH and AMVN-exposed plasma, respectively. Plasma AT and BC were extensively depleted under these oxidant conditions. The addition of CTCH prevented or delayed the formation of TBARS, and the depletion of AT and BC in a dose dependent manner. This antioxidant effect was dependent on the concentration of CTCH and on the physical characteristics of the radical initiator. CTCH supplementation modified not only the lag time for the antioxidants depletion, but also the consumption rate. These results indicate that CTCH was an effective antioxidant in human blood plasma, delaying the consumption of endogenous lipid soluble antioxidants (AT and BC) and inhibiting lipid oxidation.
Asunto(s)
Antioxidantes/farmacología , Sangre/metabolismo , Catequina/farmacología , Amidinas/farmacología , Compuestos Azo/farmacología , Sangre/efectos de los fármacos , Catequina/sangre , Cromatografía Líquida de Alta Presión , Humanos , Cinética , Peroxidación de Lípido , Nitrilos/farmacología , Oxidantes/farmacología , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Vitamina E/sangre , beta Caroteno/sangreRESUMEN
A growing amount of scientific evidence supports the participation of oxygen radicals in heart disease and, consequently, a protective effect of vitamin E (VE), beta-carotene (BC), and other antioxidants. The aim of this study was to correlate plasma VE and BC concentration with the clinical course of the acute myocardial infarction (AMI). We evaluated 120 patients that were admitted at the coronary units within 12 h after the development of AMI symptoms. The AMI was diagnosed by clinical and biochemical criteria and by electrocardiography and echocardiography. Plasma VE and BC concentration was determined by high performance liquid chromatography. The patients were separated according to the plasma concentration of VE (group H, VE > 17.5 microM; group L, VE < 17.5 microM). Clinical history of patients, age, sex, associated cardiovascular risk factors, AMI localization, hemodynamic class, and the treatment received were similar between different groups. The blood levels of creatine phosphokinase (CK) evaluated either 24- or 48-h after admittance, were higher in group L than in group H (24 h: H = 436 +/- 31 U/ml vs. L = 642 +/- 84 U/ml; p < .005; 48 h: H = 242 +/- 21 U/ml; L = 423 +/- 82 U/ml, p < 0.005). The number of deflexions in the electrocardiogram at admittance (ECG-D) was significantly higher in group L than in group H (4.7 +/- 0.3 vs. 3.7 +/- 0.2; p < .005). The number of new Q waves in the ECG of release (ECG-Q) was higher in group L than in group H (2.9 +/- 0.3 vs. 2.2 +/- 0.2; p < .05). The number of segments affected in the echocardiograms (EC-S) was: L = 5.3 +/- 0.6 vs. H = 4.4 +/- 0.2; p = 0.11. No significant differences in CK levels, ECG-D, ECG-Q, and EC-S were observed when the patients were separated according their plasma BC levels. These results indicate that a high concentration of plasma VE, but not BC, was associated with a diminution in the creatine phosphokinase release and with the AMI extension.
Asunto(s)
Antioxidantes/uso terapéutico , Lípidos/química , Infarto del Miocardio/tratamiento farmacológico , Vitamina E/uso terapéutico , Electrocardiografía/efectos de los fármacos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Infarto del Miocardio/sangre , Infarto del Miocardio/patología , Solubilidad , Vitamina E/sangreRESUMEN
A quantitative simulation model was developed that utilized present knowledge of lipid peroxidation in biological systems. The simulation model incorporated the following features: peroxidizability of polyunsaturated lipids, activation of inducers and their initiation of lipid peroxidation, concurrent autoxidation, inhibition of lipid peroxidation by vitamin E, reduction of some of the hydroperoxides by glutathione peroxidase, and formation of thiobarbituric acid-reactive substances. Simulation calculations were done using a computer spreadsheet program. When the simulation program was applied to tissue slice and microsomal peroxidizing systems, the results of the stimulation were in agreement with the experimental data.
Asunto(s)
Simulación por Computador , Peroxidación de Lípido , Derivados del Benceno/metabolismo , Sistema Enzimático del Citocromo P-450/metabolismo , Etanol/metabolismo , Hígado/metabolismo , Peróxidos/metabolismoRESUMEN
Liver slices were used to measure lipid peroxidation induced by bromotrichloromethane, tert-butyl hydroperoxide (t-BOOH), or ferrous iron. The responses of liver homogenates and microsomes to oxidative conditions were compared with the response of tissue slices. Lipid peroxidation was evaluated by the production of thiobarbituric acid-reactive substances (TBARS). As was observed in homogenates and microsomes, TBARS production by liver slices depended upon the amount of tissue, the incubation time, inducer, the amount of inducer, and the presence of antioxidant. Control liver slices incubated at 37 degrees C for 2 h produced 19 nmol of TBARS per g of liver. When slices were incubated in the presence of 1 mM BrCCl3, 1 mM t-BOOH, or 50 microM ferrous iron, TBARS production increased 4.6-, 8.2-, or 6.7-fold over the control value, respectively. Comparable induction of TBARS by liver homogenates and microsomes was observed when these preparations were incubated with the same inducers. Addition of 5 microM butylated hydroxytoluene (BHT) prevented the induction of TBARS by 50 microM ferrous iron by liver slices. The results indicate the usefulness of tissue slices to measure lipid peroxidation. The usefulness of tissue slices is emphasized when a number of compounds or tissues are studied and tissue integrity is desired as in toxicological, pharmacological, and nutritional studies where reduced numbers of experimental animals is a relevant issue.
Asunto(s)
Peróxidos Lipídicos/biosíntesis , Hígado/metabolismo , Animales , Hidroxitolueno Butilado/farmacología , Técnicas de Cultivo/métodos , Compuestos Ferrosos/farmacología , Masculino , Microsomas Hepáticos/metabolismo , Ratas , Ratas Endogámicas , TiobarbitúricosRESUMEN
Twenty-seven halogenated compounds were screened as potential inducers of lipid peroxidation in rat liver, kidney, spleen, and testes slices. In addition to the known lipid peroxidation inducers--carbon tetrachloride and bromotrichloromethane--the novel compounds carbon tetrabromide, p-bromobenzyl bromide, and benzyl bromide increased lipid peroxidation in each of the tissues studied. Lipid peroxidation was measured by release of thiobarbituric acid-reactive substances (TBARS) from the tissue slices. The amount of TBARS released from liver slices incubated with bromotrichloromethane, carbon tetrabromide, dichloromethane, bromobenzene, chloroform, bromoform, benzyl chloride, bromochloromethane, and carbon tetrabromide correlated with the lethality of these compounds as evaluated by their oral LD50 in rats. The lethality of a number of the compounds tested did not correlate with their capacity to induce lipid peroxidation.
Asunto(s)
Hidrocarburos Halogenados/farmacología , Riñón/metabolismo , Peróxidos Lipídicos/biosíntesis , Hígado/metabolismo , Bazo/metabolismo , Testículo/metabolismo , Animales , Técnicas In Vitro , Riñón/efectos de los fármacos , Hígado/efectos de los fármacos , Masculino , Ratas , Ratas Endogámicas , Bazo/efectos de los fármacos , Relación Estructura-Actividad , Testículo/efectos de los fármacosRESUMEN
We have characterized the effect of angiotensin converting enzyme (ACE) inhibitors on the activity of CuZn-superoxide dismutase (CuZn-SOD), Mn-superoxide dismutase (Mn-SOD), catalase, and selenium-dependent glutathione peroxidase (Se-GPx). CF1 mice (4-month-old females) were administered water containing enalapril (20 mg/l) or captopril (50 mg/l), during 4 to 11 weeks. After 11 weeks, enalapril treatment caused an increase in the activity of CuZn-SOD, Mn-SOD and Se-GPx, from 19 +/- 4 to 46 +/- 7, 2.1 +/- 0.2 to 3.8 +/- 0.2 units/mg protein and 27 +/- 3 to 54 +/- 3 milliunits/mg protein, respectively. After 11 weeks, captopril treatment increased the activities (P < 0.05) of CuZn-SOD, MnSOD and Se-GPx to 35 +/- 4, 2.9 +/- 0.2 units/mg protein, and 38 +/- 2 milliunits/mg protein, respectively. Catalase activity was not affected by the treatments. These results suggest that ACE inhibitors may protect cell components from oxidative damage by increasing the enzymatic antioxidant defenses.
Asunto(s)
Captopril/farmacología , Enalapril/farmacología , Glutatión Peroxidasa/metabolismo , Hígado/enzimología , Superóxido Dismutasa/metabolismo , Animales , Catalasa/metabolismo , Femenino , Hígado/efectos de los fármacos , RatonesRESUMEN
To determine nonscorbutic effects of moderate vitamin C deficiency we measured immune function and oxidative damage in eight healthy men (25-43 y) who consumed 5-250 mg/d of ascorbic acid over 92 d on a metabolic unit. During ascorbic acid intakes of 5, 10, or 20 mg/d, subjects attained a state of moderate ascorbic acid deficiency as ascorbic acid concentrations in plasma, leucocytes, semen, and buccal cells dropped to less than 50% of baseline with no scorbutic symptoms observed. No changes in cell proliferation, erythrocyte antioxidant enzymes, and DNA strand breaks were observed; however, blood levels of glutathione and NAD(P) decreased during ascorbic acid deficiency, as did delayed hypersensitivity responsiveness. Concentrations of the oxidatively modified DNA base, 8-hydroxydeoxyguanosine in sperm DNA and fecapentaenes, ubiquitous fecal mutagens, were increased during ascorbic acid depletion. Moderate vitamin C deficiency, in the absence of scurvy, results in alteration of antioxidant chemistries and may permit increased oxidative damage.
Asunto(s)
Deficiencia de Ácido Ascórbico/fisiopatología , Inmunocompetencia , Oxidantes/metabolismo , Adulto , Ácido Ascórbico/metabolismo , Deficiencia de Ácido Ascórbico/inmunología , Deficiencia de Ácido Ascórbico/metabolismo , Humanos , Masculino , Polienos/análisisRESUMEN
We previously reported chronic treatment with angiotensin-converting enzyme inhibitors (ACEis) increases antioxidant defenses in mice. In the present study, however, we examined various antioxidant defenses in chronic hemodialysis (HD) patients either treated with enalapril (10 mg/d) for at least 6 months (+ACEi; n = 11) or untreated (-ACEi; n = 11). The relationship between antioxidant status and HD was investigated by determining oxidative stress markers and antioxidant defenses in a group of chronic HD patients (n = 33) and a group of age-matched controls (n = 29). The effect of a single HD session on those parameters was also evaluated. Before an HD session (pre-HD), HD patients had significantly lower levels of red blood cell (RBC) glutathione (GSH), selenium-dependent glutathione peroxidase activity (RBC-Se-GPx), plasma ubiquinol-10, and alpha-tocopherol than controls. In a randomly selected group of patients (n = 19), a single HD session caused an additional decrease in RBC-GSH and plasma ubiquinol-10 levels. Plasma thiobarbituric acid reactive substance (TBARS) levels were significantly greater in pre-HD patients than controls. Post-HD plasma TBARS levels were similar to control values. The cohort of +ACEi HD patients had greater pre-HD RBC-GSH content, RBC-Se-GPx activity, and plasma beta-carotene concentrations than -ACEi patients (RBC-GSH: +ACEi, 3.1 +/- 0.9 micromol/mL packed RBCs [PRBCs]; -ACEi, 1.2 +/- 0.3 micromol/mL PRBCs [P < 0.05 v +ACEi]; RBC-Se-GPx: +ACEi, 5.8 +/- 0.7 U/mL PRBCs; -ACEi, 4.3 +/- 0.2 U/mL PRBCs [P < 0.05 v +ACEi]; plasma beta-carotene: +ACEi, 0.54 +/- 0.16 micromol/L plasma; -ACEi, 0.19 +/- 0.05 micromol/L plasma [P < 0.05 v +ACEi]). Results show profound alterations in the circulating antioxidant systems of chronic HD patients and that additional oxidative stress occurs during the HD procedure. In addition, in +ACEi HD patients, the levels of several antioxidant defenses are greater than in those in -ACEi HD patients.
Asunto(s)
Inhibidores de la Enzima Convertidora de Angiotensina/administración & dosificación , Antioxidantes/metabolismo , Enalapril/administración & dosificación , Fallo Renal Crónico/terapia , Peroxidación de Lípido/efectos de los fármacos , Diálisis Renal , Adulto , Anciano , Inhibidores de la Enzima Convertidora de Angiotensina/efectos adversos , Animales , Catalasa/sangre , Enalapril/efectos adversos , Eritrocitos/enzimología , Femenino , Radicales Libres , Glutatión/sangre , Glutatión Peroxidasa/sangre , Humanos , Fallo Renal Crónico/enzimología , Masculino , Malondialdehído/sangre , Ratones , Persona de Mediana Edad , Especies Reactivas de Oxígeno/metabolismo , Ubiquinona/análogos & derivados , Ubiquinona/sangre , Vitamina E/sangreRESUMEN
Administration of eriodyctiol and (+)-catechin (10 mg/100 g of body weight) to mice inhibited the enhancement of in situ liver chemiluminescence produced by CCl4 (0.5 ml/100 g) by 32 and 38% respectively. 3,4-Dicaffeoylquinic acid was less effective (13%), and cynarin had no effect. Previously, these compounds and other polyphenols were assayed as in vitro antioxidants by their abilities to inhibit the tert-butyl hydroperoxide (t-BOOH)-initiated chemiluminescence of mouse liver homogenates, and the IC50 (microM) values were as follows: (+)-catechin, 3; eriodyctiol, 9; myricetin and 4,2',4'-trihydroxy-6'-metoxychalcone, 15; 3,4-dicaffeoylquinic acid, 20; isochlorogenic acid, 30; caffeic acid, 5,6,3'-trihydroxy-7,4'-dimethoxyflavone and cynarin, 50; chlorogenic acid and apigenin, 150; quercetin, pedalitin, sylimarin and quercetin-3-methyl ester, 200; 7,4'-dihydroxy-5-methoxyflavonone and kaempferol-3,7-dirhamnoside, 500; quercitrin, 900; and galangin-3-methyl ether, genkwanin, hesperidin, ombuoside, phloridzin, quinic acid, rhoifolin, rutin and sophoricoside, greater than 1 mM. The in vitro and in vivo effects of these flavonoids and polyphenols may be related to their antioxidant abilities, making them promising substances to be investigated as water-soluble protectors against lipid peroxidation and other free radical-mediated cell injury.
Asunto(s)
Antioxidantes/farmacología , Flavonoides/farmacología , Hígado/efectos de los fármacos , Animales , Tetracloruro de Carbono/toxicidad , Femenino , Peróxidos Lipídicos/metabolismo , Mediciones Luminiscentes , Ratones , Fenoles/farmacología , Polímeros/farmacología , Relación Estructura-ActividadRESUMEN
Twenty six phenolic substances including representatives of the families, flavanones, flavanols and procyanidins, flavonols, isoflavones, phenolic acids and phenylpropanones were investigated for their effects on lipid oxidation, membrane fluidity and membrane integrity. The incubation of synthetic phosphatidylcholine (PC) liposomes in the presence of these phenolics caused the following effects: (a) flavanols, their related procyanidins and flavonols were the most active preventing 2,2'-azo-bis (2,4-dimethylvaleronitrile) (AMVN)-induced 2-thiobarituric acid-reactive substances (TBARS) formation, inducing lipid ordering at the water-lipid interface, and preventing Triton X-100-induced membrane disruption; (b) all the studied compounds inhibited lipid oxidation induced by the water-soluble oxidant 2,2'-azo-bis (2-amidinopropane) (AAPH), and no family-related effects were observed. The protective effects of the studied phenolics on membranes were mainly associated to the hydrophilicity of the compounds, the degree of flavanol oligomerization, and the number of hydroxyl groups in the molecule. The present results support the hypothesis that the chemical structure of phenolics conditions their interactions with membranes. The interactions of flavonoids with the polar head groups of phospholipids, at the lipid-water interface of membranes, should be considered among the factors that contribute to their antioxidant effects.
Asunto(s)
Antioxidantes/farmacología , Flavonoides/farmacología , Fluidez de la Membrana/efectos de los fármacos , Membranas/efectos de los fármacos , Amidinas/antagonistas & inhibidores , Amidinas/química , Compuestos Azo/antagonistas & inhibidores , Compuestos Azo/química , Flavonoides/química , Membrana Dobles de Lípidos/química , Lípidos/química , Liposomas/química , Membranas/química , Micelas , Nitrilos/antagonistas & inhibidores , Nitrilos/química , Oxidantes/antagonistas & inhibidores , Oxidación-Reducción/efectos de los fármacos , Relación Estructura-Actividad , Sustancias Reactivas al Ácido Tiobarbitúrico/químicaRESUMEN
To assess the effect of chronic ethanol ingestion in the content of the reduced forms of coenzymes Q9 (ubiquinol-9) and Q10 (ubiquinol-10) as a factor contributing to oxidative stress in liver and brain, male Wistar rats were fed ad libitum a basal diet containing either 10 or 2.5 mg alpha-tocopherol/100 g diet (controls), or the same basal diet plus a 32% ethanol-25% sucrose solution. After three months treatment, ethanol chronically-treated rats showed identical growth rates to the isocalorically pair-fed controls, irrespectively of alpha-tocopherol dietary level. Lowering dietary alpha-tocopherol led to a decreased content of this vitamin in the liver and brain of control rats, without changes in that of ubiquinol-9, and increased levels of hepatic ubiquinol-10 and total glutathione (tGSH), accompanied by a decrease in brain tGSH. At the two levels of dietary alpha-tocopherol, ethanol treatment significantly decreased the content of hepatic alpha-tocopherol and ubiquinols 9 and 10. This effect was significantly greater at 10 mg alpha-tocopherol/100 g diet than at 2.5, whereas those of tGSH were significantly elevated by 43% and 9%, respectively. Chronic ethanol intake did not alter the content of brain alpha-tocopherol and tGSH, whereas those of ubiquinol-9 were significantly lowered by 20% and 14% in rats subjected to 10 and 2.5 mg alpha-tocopherol/100 g diet, respectively. It is concluded that chronic ethanol intake at two levels of dietary alpha-tocopherol induces a depletion of hepatic alpha-tocopherol and ubiquinols 9 and 10, thus contributing to ethanol-induced oxidative stress in the liver tissue. This effect of ethanol is dependent upon the dietary level of alpha-tocopherol, involves a compensatory enhancement in hepatic tGSH availability, and is not observed in the brain tissue, probably due to its limited capacity for ethanol biotransformation and glutathione synthesis.
Asunto(s)
Encéfalo/efectos de los fármacos , Etanol/administración & dosificación , Hígado/efectos de los fármacos , Ubiquinona/análogos & derivados , Ubiquinona/metabolismo , Vitamina E/administración & dosificación , Animales , Encéfalo/metabolismo , Dieta , Glutatión/metabolismo , Hígado/metabolismo , Masculino , Oxidación-Reducción , Estrés Oxidativo , Ratas , Ratas WistarRESUMEN
Oxidative stress parameters were evaluated in rat testes after chronic iron intoxication and vitamin E supplementation. Male Wistar rats were fed during 6 weeks with the following diets: C = rat chow; I = C + 25 mg carbonyl-iron/g diet; A = C + 0.2 mg alpha-tocopheryl acetate/g diet; and the combination of I and A (IA). After the treatment, no changes in final body weight, testis weight and protein content were observed. Total iron content in testes from the I group was 33% higher compared to the C group (216 +/- 10 nmol/g of tissue). The content of alpha-tocopherol (alphaT) was 2.5-fold higher in the A and IA groups compared to the C group (12.8 +/- 0.7 nmol/g tissue). The content of ubiquinol-9 (13.0 +/- 1.7 nmol/g tissue) and ubiquinol-10 (3.3 +/- 0.5 nmol/g tissue) was similar among the groups. Superoxide dismutase activity was 13 and 16% lower in the A and IA groups with respect to the C group (12.9 +/- 0.7 U/mg protein). Catalase activity was 26 and 33% lower in the I and IA groups than in the C (0.19 +/- 0.01 pmol/mg protein) and A (0.21 +/- 0.01 pmol/mg protein) groups, respectively. Glutathione peroxidase was 24 and 23% higher in the IA group than in the C (11.4 +/- 0.3 mU/mg protein) and I (11.5 +/- 1.0 mU/mg protein) groups, respectively. The testes content of 2-thiobarbituric acid-reactive substances (TBARS) and protein-associated carbonyl groups were 37 and 16% higher, respectively, in the I group than in the C group. These increased in TBARS and carbonyls, were not observed in the IA group. No diet-associated changes were observed in the steady state levels of 8-oxo-2'-deoxyguanosine in testes DNA (4.2 +/- 0.2 residue/10(5) dG). The present data suggest that this model of chronic iron overload produced a mild oxidative damage in rat testes that was partially prevented by alphaT supplementation.
Asunto(s)
Hierro/envenenamiento , Estrés Oxidativo , Testículo/metabolismo , Vitamina E/farmacología , Animales , ADN/metabolismo , Hierro/metabolismo , Peroxidación de Lípido , Masculino , Oxidación-Reducción , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismoRESUMEN
Excessive dietary aluminum (Al) has been proposed to be a factor contributing to several neurological disorders in humans. Six 8-week-old female Swiss Webster mice were fed for 10 weeks purified diets containing 100 (control, 100 Al), 500 (500 Al) or 1000 (1000 Al) micrograms Al/g diet. Brain and liver lipid peroxidation was determined by evaluating the production of 2-thiobarbituric acid reactive substances (TBARS) in brain and liver homogenates in the presence or absence of 50 microM ferrous iron. TBARS production in the absence of iron in brain homogenates from mice fed the 1000 Al diet was higher (30%) than that in the 100 Al control group (3.1 vs. 2.4 nmol TBARS/mg protein). The addition of ferrous iron increased TBARS production in brain homogenates from all 3 dietary groups. The iron-induced TBARS production was 26% higher in the 1000 Al brain homogenates than in the 100 Al group (4.9 vs. 3.9 nmol TBARS/mg protein). Brain TBARS production in the presence and absence of iron was similar between the 100 and 500 Al groups. TBARS production in liver homogenates measured either with or without iron was similar for the 3 groups. These results show that, in mice, dietary Al intoxication leads to increased brain TBARS production, suggesting that enhanced lipid peroxidation may be one possible mechanism underlying the neurological damage associated with increased tissue Al.
Asunto(s)
Aluminio/toxicidad , Encéfalo/efectos de los fármacos , Peroxidación de Lípido/efectos de los fármacos , Tiobarbitúricos/análisis , Aluminio/administración & dosificación , Animales , Encéfalo/metabolismo , Dieta , Femenino , Hierro/metabolismo , Hígado/efectos de los fármacos , Hígado/metabolismo , Ratones , Tiobarbitúricos/farmacología , Factores de TiempoRESUMEN
Our previous studies have shown that men with low ascorbate intake have markedly increased oxo8dG in the DNA of their sperm. Because cigarette smoke is high in oxidants and depletes plasma and tissue antioxidants, oxidative DNA damage in sperm and tocopherol and ascorbate levels in seminal plasma were determined in smokers and non-smokers. The level in sperm DNA of oxo8dG, an oxidative lesion of guanine, was 50% higher in smokers compared to nonsmokers (p = 0.005). The concentration of alpha-tocopherol in seminal plasma was decreased in smokers by 32% (p = 0.03). Smoking and low antioxidant levels increase oxidative damage to sperm DNA. We discuss the possibility that paternal smoking causes mutations in sperm that lead to cancer, birth defects, and genetic diseases in offspring.
Asunto(s)
Antioxidantes/análisis , Daño del ADN , Desoxiguanosina/análogos & derivados , Fumar/efectos adversos , Espermatozoides/química , 8-Hidroxi-2'-Desoxicoguanosina , Adolescente , Adulto , Argentina , California , Desoxiguanosina/análisis , Humanos , Masculino , Persona de Mediana Edad , Oxidación-Reducción , Semen/químicaRESUMEN
Red blood cell membranes (RBCM) were used to estimate human red blood cell lability to lipid peroxidation in vitro. RBCM were prepared from blood collected from humans fed diets with either 3 or 15% polyunsaturated fatty acids for 80 days. RBCM were isolated by centrifugation, and oxidative stress was induced by in vitro incubation with 0.1 or 0.5 mM tert-butyl hydroperoxide (t-BOOH) in the presence of 0.5 mg added hemoglobin. Lipid Peroxidation was evaluated by measurement of thiobarbituric acid-reactive substances (TBARS). Lipid peroxidation correlated with the protein content of RBCM in both noninduced and t-BOOH-induced lipid peroxidation systems. TBARS production was dependent on the amount of t-BOOH added to the RBCM. The production of TBARS by RBCM incubated with 0.5 mM t-BOOH was correlated with arachidonic acid content in the red blood cells (RBC) from which RBCM were prepared. The methodology developed was useful for comparative estimations of the lability of RBCM to lipid peroxidation.
Asunto(s)
Grasas Insaturadas en la Dieta/farmacología , Membrana Eritrocítica/metabolismo , Ácidos Grasos Insaturados/farmacología , Peroxidación de Lípido/efectos de los fármacos , Lípidos de la Membrana/sangre , Adulto , Humanos , Masculino , Persona de Mediana Edad , Tiobarbitúricos , Vitamina E/farmacologíaRESUMEN
We evaluated the antioxidant effect of (+)-catechin (CTCH), in the presence of physiological antioxidant levels of ascorbic acid (AA), alpha-tocopherol (AT) and beta-carotene (BC), in human plasma oxidised with AAPH. Following a five-hour incubation, the formation of lipid oxidation products (TBARS) was almost doubled, and the concentrations of lipid soluble antioxidants were 10 to 30% from the initial levels. In these conditions, AA was consumed within the first hour of incubation. The addition of CTCH prevented AT and BC depletion and TBARS formation, but had no effect on AA consumption. When the kinetics of oxidation were analysed CTCH oxidation preceded lipid soluble antioxidant depletion, but no consumption of CTCH was associated to AA oxidation. Considering that CTCH could contribute to the antioxidant activity of red wine, we first characterised both the antioxidant capacity and CTCH content of several wines. The wines with highest content of CTCH and antioxidant activity were also the most effective in preventing AAPH-mediated oxidation of plasma vitamin E. Results support the idea that CTCH could have a role as a physiological antioxidant in human plasma, and that CTCH of wine could contribute to the antioxidant status of human plasma.