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Objective Identification of viral replication in patients with HB without HBeAg is significant for estimating course and adopting appropriate therapy.This research aimed to clarify clinical signification of detecting Pre-S1Ag for identifing viral replication in patients with HB without HBeAg.Methods Sera samples were collected from 65 outpatients with HB confirmed by liver biopsy. HBV markers ,Pre-S1 antigen in sera were detected by ELISA;HBV-DNA by PCR;HBsAg, HBcAg and Pre-S1Ag in tissue by immunohistochemistry method.Results Intracellular HBsAg,Pre-S1Ag mainly expressing in the plasma account for 78.4% (51/65) ,69.2%(45/65) respectively, HBcAg partly in the plasma and the nucleus account for 67.6(44/65).Positive rate of expressing HBcAg,Pre-S1Ag are little less than HBsAg, but very near between Pre-S1Ag and HBcAg.There are no significant difference by ?~2 test (P=0.736). There are no significant difference between HBV-DNA (≥10~3copies/ml) and Pre-S1Ag(P=0.059),but there are significant difference HBV-DNA-positive are 18, in which 14 are Pre-S1Ag-positive.It demonstrates that there are viral replication in 18 of 30 HBeAg-negative patients and Pre-S1Ag corresponds considerably with HBV-DNA. Among 14 HBeAg-negative patients there are 4 cases with Pre-S1Ag without HBeAg because of Pre-C mutation.Conclusion Pre-S1 is a valuably serological marker for HBV replication without HBeAg.
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Objective To observe the changes of specific proteins in serum of acute stage patients with SARS and its clinical application. Methods The acute stage patients with SARS group included 42 cases who had clinical symptoms less than five days. In normal group: 30 healthy adults were as controls. Tests for specific proteins were immunoglobin G ( IgG ) , immunoglobin A ( IgA) , immunoglobin M ( IgM) , complement C3( C3),complement C4(C4),C-reaction protein (CRP), al acid glycoprotein( a1-AG) , a1 antitrypsin(a1-AT) ,transferrin(TRF), ceruloplasmin( CER) in sera by Ratenephelometer. Results The level of CRP, a1-AG, a1-AT in sera of patients with SARS were higher than that in control group ( P 0. 05). Conclusion It suggested that specific proteins in serum of acute stage patients with SARS change in a particular way. There were some help for the diagnosis of the phlogistic degree of acute stage patients with SARS by detecting CRP, a1-AG and TRF simultaneously.
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0.05), while those of HBV DNA and ALT coincided poorly (P