Diagnostic features of atherosclerosis development in miners having anthracosilicosis.

To design forecasting system, the authors studied occurrence of atherosclerosis and its risk factors in 152 workers of coal mines in South Kouzbass (tunnellers, cleaning pit-face miners, mining excavator operators), suffering from anthracosilicosis. Atherosclerosis was revealed in 124 (81.6%) workers with anthracosilicosis. With Bayess method for independent signs and Wald's sequential analysis method, the authors created a way to forecast atherosclerosis with coronary, extracranial and peripheral arteries involvement in miners with anthracosilicosis. Maximal risk of atherosclerosis in miners with anthracosilicosis is associated with following parameters: age 45 years and over, arterial hypertension, smoking, abdominal obesity type, diabetes mellitus, metabolic syndrome, respiratory failure, family history of IHD, hypercholesterolemia, increased LDL cholesterol, decreased HDL cholesterol, increased atherogeneity coefficient, hypertriglyceridemia, hyperhomocysteinemia, hyperfibrinogenemia, increased C-reactive protein, hypersthenic type according to Reese-Isenc index, andromorphic type according to Tanner index, blood group. markers A (II) and B (III), rhesus negative, MN and NN. Through increased number of factors analyzed and selected additional markers, accuracy of atherosclerosis forecasting is increased - that enables to proceed with opportune treatment and prevention.

[Analysis of Eight Polymorphic Alu Elements in the Teleuts Population].

Allele frequencies and genetic diversity in the population of Teleuts were assessed by the Alu repeat polymorphism at eight autosomal loci (ACE, APOA1, PLAT, F13, PV92, A25, CD4, Dl). For comparison, the study included previously obtained data on the Alu polymorphism in 19 indigenous populations of Siberia. On the dendrogram of genetic distances, the Teleut population is located in the cluster of Siberian ethnic groups, which are similar in origin, geography, and cultural traditions.

[Genotoxic effects of occupational environment in Kuzbass miners].

The authors studied chromosomal aberrations in blood lymphocytes of 100 miners in coal mines of Kuznetsk coal field, with underground length of service over 15 years. Reference data were collected by cytogenetic analysis in a group of workers with long length of service in Kemerovo thermal power plant, contacting coal dust (n = 104) and in healthy males of the same age group, residents of Kemerovo city, without occupational contact with mutagenes (n = 194). The miners appeared to have maximal frequency of structural chromosomal injuries--5.37%. In the workers of thermal power plant, this value was considerably lower than in the miners (4.23%; p < 0.01), but not exceeding the values obtained in the reference groups (1.07%; p < 0.0001). Analysis of qualitative spectrum of chromosomal structural changes revealed that the miners have higher frequency of both chromosomal and chromatid type aberrations. Differences in chromosomal aberrations frequency between the miners and the thermal power plant workers are mostly due to chromosomal type aberrations. This points to significant role of chemical and radiation factors contribution into chromosomal mutagenesis in miners.

[Diagnosis of predisposition to chronic cor pulmonale formation in occupational lung diseases caused by dust].

Study covered 426 miners aged 40-54 years with previously diagnosed occupational respiratory diseases due to dust (246 patients with chronic occupational obstructive bronchitis, 98 with anthracosilicosis and 82 with chronic dust nonobstructive bronchitis). 315 (73.9%) examinees out of 426 with lung diseases due to dust demonstrated chronic cor pulnmonale. Considering high share of this complication, the authors used Bayes method to create a method to diagnose predisposition towards chronic cor pulmonale in patients with dust lung diseases through respiratory failure, concomitant coronary heart disease and arterial hypertension, blood groups ABO, MN and P, some structural and functional parameters of heart: myocardium weight index, relative wall thickness index and left ventricle sphericity index, average lung artery pressure. Increasing number of analyzed factors that directly influence chronic cor pulmonale development and selecting additional markers help to improve forecasting of the complication.

[The role of genetic factors in the development of chronic dust bronchitis in workers of coal mining enterprises of Kuzbass].

The distribution of genotypes of HP, GC, EsD, AsP and polymorphisms GSTT1 (GST-theta1) and GSTM1 (GST-micro1) and NOS3 (polymorphism VNTR4) in miners with chronic dust bronchitis, and in those without this occupational disease has been studied The carriers of genotypes of genotypes EsD 1-2, AsP bb were shown to be more prone to develop chronic dust bronchitis. Endogenous factors of resistance to the disease are the genotypes GC 1-1, EsD 1-1, AsP bc.

[Chronic occupational bronchitis in workers of coal extracting enterprises in Kouzbass: role of endogenous factors].

The authors studied distribution of biochemical markers for HP, GC, EsD, AcP genes, polymorphism of GSTT1 (GST-theta 1), GSTM1 (GST-mu 1), locus WNTR of NOS3 gene (alleles A/B) in chronic dust bronchitis patients and in apparently healthy individuals. Genotypes EsD 1-2 and AcP bb individuals were proved to be most prone to the disease. Endogenous resistent factors for chronic dust bronchitis are genotypes GC 1-1, EsD 1-1, AcP bc.

[Comparative characteristics of the gene pool of Teleuts inferred from Y-chromosomal marker data].

The gene pool structure of Teleuts was examined and Y-chromosomal haplogroups composition and frequencies were determined. In the gene pool of Teleuts, five haplogroups, C3xM77, N3a, R1b*, R1b3, and R1a1, were identified. Evaluation of the genetic differentiation of the samples examined using analysis of molecular variance (AMOVA) with two marker systems (frequencies of haplogroups and Y-chromosomal microsatellite haplotypes) showed that Bachat Teleuts were equally distant from Southern and Northern Altaians. In Siberian populations, the frequencies and molecular phylogeny of the YSTR haplotypes within Y-chromosomal haplogroup R1a1 were examined. It was demonstrated that Teleuts and Southern Altaians had very close and overlapping profiles of R1a1 haplotypes. Population cluster analysis of the R1a1 YSTR haplotypes showed that Teleuts and Southern Altaians were closer to one another than to all remaining Siberian ethnic groups. Phylogenetic analysis of N3a haplotypes suggested specificity of Teleut haplotypes and their closeness to those of Tomsk Tatars. Teleuts were characterized by extremely high frequency of haplogroup R1b*, distinguished for highly specific profile of YSTR haplotypes and high haplotype diversity. The results of the comparative analysis suggested that the gene pool of Bachat Teleuts was formed on the basis of at least two heterogeneous genetic components, probably associated with ancient Turkic and Samoyedic ethnic components.

[Immune genetic markers of anthracosilicosis].

Studies of ABO, Rh, MN, rho, Lewis blood groups in coal miners suffering from anthracosilicosis and in reference healthy individuals proved that MM phenotypes are predisposed to anthracosilicosis and MN phenotypes could be resistant to the disease. These results could be valuable for prevention of this occupational disease among coal industry workers.

[Genetic characteristics of the population of Severo-Baikal'skii region of the northern part of the Buryat Republic]. / Genetikcheskaia kharakteristika naseleniia Severo-Baikal'skogo raiona Severa Buriatii.

In the populations of seven settlements of Severo-Baikal'skii raion (Buryat Republic), 12 genetic systems were studied: ABO, Rh, MN, P, Lewis, HP, GC, TF, PI, Gm1, ABH, and the cerumen consistency. With respect to most of these genetic systems, the studied populations were either insignificantly different from other Siberian populations or intermediate between Russian populations from the European part of Russia and from indigenous Cis-Baikal populations (Buryats and Evenks). This might be related to outbreeding and selection. The observed parameters of genetic differentiation indicated that the studied system of subpopulations was at an equilibrium, with insignificant differences between subpopulations.

[Hereditary polymorphism features in patients with silicosis associated with chronic bronchitis]. / Osobennosti nasledstvennogo polimorfizma u bol'nykh silikozom s soputstvuiushchim khronicheskim bronkhitom.

Chronic bronchitis frequently accompanies silicosis in metallurgy workers. Association of the two diseases significantly lowers life quality and disables the patients. Prophylactic measures for the diseases are elimination of the etiologic factors and detection of genetic markers for propensity and insusceptibility to silicosis and chronic bronchitis. Phenotypes of Lewis a-b- system, of Hp2-1 haptoglobin and of group-specific component Gc2-2 could be risk factors for chronic bronchitis in silicosis patients. Those of Lewis a-b+ system, of Hp2-2 haptoglobin and group-specific component Gc1-2 are resistant to chronic bronchitis on silicosis background.