RESUMEN
Six cases of fatal myocarditis associated with encephalomyocarditis virus occurred over a 14-month period in a group of outdoor-housed juvenile rhesus macaques. All animals were younger than 3 years of age and died or were euthanized following acute onset of dyspnea or pulmonary effusion (3 of 6) or were found dead without premonitory signs (3 of 6). Gross findings included pulmonary congestion (6 of 6), variable degrees of pleural effusion (4 of 6), multifocal pale tan foci throughout the myocardium (3 of 6), hepatomegaly and hepatic congestion (3 of 6), and pericardial effusion (1 of 6). Histologically, affected myocardium was infiltrated multifocally by lymphoplasmacytic and histiocytic inflammation admixed with necrotic and degenerate myofibers and infrequent mineralization (6 of 6). Pulmonary edema was present in all animals. Encephalomyocarditis virus was confirmed in 6 of 6 hearts by immunohistochemistry, and virus was isolated from one case by polymerase chain reaction. Sequencing of virus isolated from 1 affected animal indicated infection with a novel encephalomyocarditis virus. Encephalomyocarditis virus should be considered as a differential etiology in outbreaks of myocarditis and pulmonary edema in juvenile primates.
Asunto(s)
Infecciones por Cardiovirus/veterinaria , Virus de la Encefalomiocarditis/aislamiento & purificación , Macaca mulatta , Enfermedades de los Monos/virología , Miocarditis/veterinaria , Animales , Infecciones por Cardiovirus/patología , Infecciones por Cardiovirus/virología , Chlorocebus aethiops , ADN Complementario/química , ADN Complementario/genética , Brotes de Enfermedades/veterinaria , Virus de la Encefalomiocarditis/genética , Femenino , Humanos , Masculino , Microscopía Electrónica de Transmisión/veterinaria , Enfermedades de los Monos/patología , Miocarditis/patología , Miocarditis/virología , Miocardio/patología , Miocardio/ultraestructura , Edema Pulmonar/patología , Edema Pulmonar/veterinaria , Edema Pulmonar/virología , ARN Viral/genética , Análisis de Secuencia de ADN , Células VeroRESUMEN
BACKGROUND: Pediatric hepatic angiosarcoma is a rare condition in children with poor prognosis. Microscopically this neoplasm has a particular 'Kaposi-form' arrangement. Hemangiosarcoma in non-human primates is a rare finding. METHODS: Gross and microscopic examination of a 3-year-old rhesus were performed. Immunohistochemistry was used to characterize the hepatic hemangiosarcoma. RESULTS: The gross necropsy revealed hemoabdomen and a 4 x 3 x 3 cm mass in the liver with multiple smaller masses throughout the hepatic parenchyma. Histopathology confirmed a poorly differentiated hemangiosarcoma. Other organs submitted were free of metastases. CONCLUSIONS: Hemangiosarcoma in non-human primates has been rarely reported. Diagnosis was confirmed by expression of endothelial-specific markers CD31 and vWF by immunohistochemistry. Due to the young age of this monkey and the particular solid pattern throughout the mass this neoplasm resembles pediatric hepatic angiosarcoma in humans.