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1.
Bratisl Lek Listy ; 117(2): 77-9, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-26830036

RESUMEN

OBJECTIVE: Autism spectrum disorders (ASDs) are a severe group of neurodevelopmental disorders that are characterized by impairment in social communication, and imagination and social interaction. The aetiology of autism is complex, but some studies suggest autoimmunity to the central nervous system in the pathogenesis. The aim of this study is to investigate the positivity of antineuronal antibodies including anti-glutamic acid decarboxylase antibodies (anti-GAD), anti-glutamate receptor (anti-GluR) antibodies and seven types of anti-ganglioside antibodies, in children with autism. METHODS: We conducted the study over a period of one year from May 2012 to December 2013. Human anti-GAD in serum were investigated with ELISA; human autoantibodies against the N-methyl-D-aspartate subtype of GluR were investigated with indirect immunofluorescence test; class IgG antibodies against the seven gangliosides were investigated with immunoblot assay. RESULTS: Serum antineuronal antibodies were measured in 42 children (24 male, 18 female) with autism in comparison to 21 (13 male, 8 female) healthy-matched children aged between 2-12 years. There was no seropositivity of antineuronal antibodies in either of the groups. CONCLUSION: There is no evidence to support an association between autism and antibodies positivity of anti-GAD, anti-GluR and anti-gangliosides (Ref. 26).


Asunto(s)
Trastorno Autístico/inmunología , Autoanticuerpos/sangre , Sistema Nervioso Central/inmunología , Gangliósidos/inmunología , Glutamato Descarboxilasa/inmunología , Receptores de Glutamato/inmunología , Trastorno Autístico/diagnóstico , Niño , Preescolar , Manual Diagnóstico y Estadístico de los Trastornos Mentales , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Masculino , Estadística como Asunto , Transmisión Sináptica/inmunología , Ácido gamma-Aminobutírico/inmunología
2.
Clin Microbiol Infect ; 13(10): 1023-5, 2007 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-17608812

RESUMEN

The aims of this study were to detect BRO beta-lactamase types and to evaluate any correlation with the susceptibility patterns of 90 clinical isolates of Moraxella catarrhalis. The overall prevalences of the bro-1 and bro-2 genes were 78% and 12%, respectively. Penicillin G MICs for BRO-1+ isolates were significantly higher than those for BRO-2+ isolates. All the isolates were susceptible to amoxycillin-clavulanate, levofloxacin and cefixime. Resistance to clarithromycin, tetracycline and trimethoprim-sulphamethoxazole was 1.1%, 2.2% and 1.1%, respectively. One-step, length-based PCR was an efficient method to screen for BRO beta-lactamase genes.


Asunto(s)
Antibacterianos/farmacología , Moraxella catarrhalis/efectos de los fármacos , Infecciones por Moraxellaceae/microbiología , beta-Lactamasas/genética , beta-Lactamas/farmacología , ADN Bacteriano/análisis , ADN Bacteriano/genética , Farmacorresistencia Bacteriana , Humanos , Pruebas de Sensibilidad Microbiana , Moraxella catarrhalis/enzimología , Moraxella catarrhalis/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , beta-Lactamasas/clasificación , beta-Lactamasas/metabolismo
3.
Clin Microbiol Infect ; 7(7): 369-72, 2001 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-11531983

RESUMEN

The objective of this study was to compare the performances of the standard aerobic bottle (StAe), FAN aerobic (FANAe) and enhanced FAN aerobic (E-FANAe) (the charcoal component of the FANAe was revised recently to improve the feasibility of Gram smear interpretation) blood culture bottles for BacT/Alert system for the detection of Brucella melitensis in simulated blood culture. Triplicate strains of eight clinical isolates of B. melitensis were studied. Each bottle was inoculated with 5 mL of freshly collected human blood at three different targeted bacterial inocula (10(1), 10(2) and 10(3) CFU/bottle). All bottles were monitored for up to 21 days or until they became positive. The results of time to detection (TTD) on the eight B. melitensis samples were as follows: at 10(1) CFU/bottle, the E-FANAe had a mean TTD significantly shorter than the StAe (48 h vs. 56.2 h, P < 0.05); and at 10(3) CFU/bottle, the FANAe and E-FANAe had a mean TTD significantly shorter than the StAe (41.2 h and 40 h vs. 45.6 h, P < 0.05). The reproducibilities (no.of positive signals/no.of all bottles) of three bottle systems were as follows: at 10(1) CFU/bottle, the reproducibilities of StAe, FANAe and E-FANAe were 96, 83 and 58%, respectively. At 10(3) CFU/bottle, the reproducibilities of StAe, FANAe and E-FANAe were 95, 95 and 91%, respectively. Positive results for the presence of bacteria in Gram smears were confirmed in 68% of StAe, 54% of FANAe and 90% of E-FANAe. In case of suspected brucellosis, the combination of one StAe bottle and one E-FANAe bottle seems to provide the highest and fastest recovery of the organism.


Asunto(s)
Bacteriemia/diagnóstico , Brucella melitensis/aislamiento & purificación , Brucelosis/diagnóstico , Bacteriemia/microbiología , Técnicas Bacteriológicas , Brucella melitensis/crecimiento & desarrollo , Brucelosis/microbiología , Recuento de Colonia Microbiana , Estudios de Evaluación como Asunto , Humanos , Reproducibilidad de los Resultados
4.
Eur J Gynaecol Oncol ; 24(2): 157-9, 2003.
Artículo en Inglés | MEDLINE | ID: mdl-12701968

RESUMEN

PURPOSE: To investigate the frequency of human papillomavirus (HPV) infection among low-risk women for cervical cancer in our region. METHODS: In one year period, 230 consecutive women at low risk of developing cervical cancer were enrolled to the study. HPV DNA testing was performed by Hybrid Capture-I System (HC-I) and groups were constituted by HPV-positive and HPV-negative women. A comparison of the groups according to age, obstetric history and age at the beginning of sexual intercourse was made. Statistical analysis was performed. RESULTS: The frequency rate of HPV infection was demonstrated to be 6.1% (n = 14) in our study (5.9% in women < or = 45 years and 7.7% in women > 45 years). Age-dependent differences were not observed between groups. There was no significant difference between HPV-positive and negative women regarding obstetric characteristics and mean age at first intercourse. CONCLUSION: This study provided significant information on the frequency of HPV infection of low-risk women in our region. When considered with studies performed in other countries, our study may give some help on the natural history of HPV infection and cervical squamous lesions.


Asunto(s)
Papillomaviridae , Infecciones por Papillomavirus/epidemiología , Neoplasias del Cuello Uterino/epidemiología , Adulto , Colposcopía , Femenino , Humanos , Persona de Mediana Edad , Infecciones por Papillomavirus/patología , Prevalencia , Factores de Riesgo , Turquía/epidemiología , Neoplasias del Cuello Uterino/patología , Neoplasias del Cuello Uterino/virología
5.
Eur J Endocrinol ; 164(2): 277-84, 2011 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-21097568

RESUMEN

OBJECTIVE: Vascular endothelial growth factor (VEGF) and VEGF receptor 1 (VEGFR1) are known to be related to thyroid tumorigenesis. The aim of the study was to examine the expressions and serum levels of VEGF, VEGFR1, IGF1, and IGF1 receptor (IGF1R) in patients with differentiated thyroid carcinoma (DTC) compared with patients with nodular goiter (NG). METHODS: We examined 39 patients with DTC who had a clinical history of at least 2 years and compared them with 25 patients who had a pathological diagnosis of NG after thyroidectomy. Serum VEGF, VEGFR1, and IGF1 levels were measured in both patient groups. The expressions of VEGF, VEGFR1, IGF1, and IGF1R were analyzed by the immunohistochemical method in the paraffin blocks of patients' thyroidectomy samples of the patients. RESULTS: The immunostainings scores for VEGF, VEGFR1, IGF1, and IGF1R were found to be higher in patients with DTC than in those with NG. Only VEGFR1 expression was related to lymph node metastasis at the time of surgery. None of the expressions were related to the long-term prognosis of the patients. Serum VEGF was found to be higher in patients with progressive DTC than in patients in clinical remission. CONCLUSION: The expressions of VEGF and VEGFR1 were shown to be correlated with the expression of IGF1 and IGF1R. VEGFR1 expression may be an important index for the presence of lymph node metastasis at the time of thyroidectomy. Increased serum levels of VEGF may reflect disease recurrence in DTC.


Asunto(s)
Carcinoma Papilar/metabolismo , Metástasis Linfática/patología , Neoplasias de la Tiroides/metabolismo , Factor A de Crecimiento Endotelial Vascular/sangre , Receptor 1 de Factores de Crecimiento Endotelial Vascular/metabolismo , Adulto , Anciano , Análisis de Varianza , Carcinoma Papilar/patología , Carcinoma Papilar/cirugía , Progresión de la Enfermedad , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Inmunohistoquímica , Factor I del Crecimiento Similar a la Insulina/metabolismo , Masculino , Persona de Mediana Edad , Pronóstico , Estadísticas no Paramétricas , Neoplasias de la Tiroides/patología , Neoplasias de la Tiroides/cirugía , Tiroidectomía
6.
Mycoses ; 43(7-8): 293-7, 2000 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-11036399

RESUMEN

A comparative evaluation of the Etest and the broth microdilution methods for antifungal susceptibility testing of 102 clinical yeast isolates against amphotericin B, fluconazole, itraconazole, and ketoconazole was conducted. The agreements between the Etest and the broth microdilution methods were 93.1% for amphotericin B 85.2% for ketoconazole, 82.3% for itraconazole and 79.4% for fluconazole. These results suggest that the Etest approach to antifungal susceptibility testing may be a viable alternative to the NCCLS reference methods for testing yeasts, but that further evaluations are needed.


Asunto(s)
Antifúngicos/farmacología , Pruebas de Sensibilidad Microbiana/métodos , Candida/efectos de los fármacos , Rhodotorula/efectos de los fármacos
7.
Mycoses ; 43(1-2): 65-6, 2000.
Artículo en Inglés | MEDLINE | ID: mdl-10838850

RESUMEN

We report a case of Aspergillus flavus pericarditis treated with fluconazole for oral candidosis. The patient with acute myeloblastic leukaemia developed tachypnoea after antileukaemic chemotherapy. Pericardial effusion was seen in the echocardiogram. Aspergillus flavus was isolated from the pericardial fluid. The patient died from aspergillosis, before the antimycotic treatment could be changed to amphotericin B.


Asunto(s)
Aspergilosis/complicaciones , Aspergillus flavus/aislamiento & purificación , Leucemia Mieloide Aguda/complicaciones , Pericarditis/complicaciones , Aspergilosis/microbiología , Niño , Resultado Fatal , Humanos , Masculino , Pericarditis/microbiología
8.
Eur Surg Res ; 36(3): 136-41, 2004.
Artículo en Inglés | MEDLINE | ID: mdl-15178901

RESUMEN

BACKGROUND: Bacterial translocation (BT) has been suggested to be responsible for the high incidence of infections occurring after acute pancreatitis (AP). The aim of this study was to investigate the effects of the platelet-activating factor (PAF) inactivator, recombinant PAF-acetylhydrolase (rPAF-AH), and the PAF receptor antagonist, BN 52021, in AP. METHODS: Forty-eight male Wistar rats were divided into 4 groups: the sham group received saline intraperitoneally every hour for 6 h; the control group received cerulein 50 g/kg i.p. every hour for 6 h; the rPAF-AH group received AP plus rPAF-AH (5 mg/kg i.v. bolus), and the BN52021 group received AP plus BN 52021 (5 mg/kg i.v. bolus). The animals were sacrificed 12 h after the first cerulein injection. RESULTS: Supramaximal cerulein stimulation induced an increase in serum pancreatic enzymes, interleukin (IL)-6, pancreatic edema, and produced histologic evidence of AP. Compared with the control group, the addition of PAF receptor antagonists had a significant effect on serum pancreatic enzymes, pancreatic edema, and the histologic score of the pancreatitis. AP caused significant increases in BT in mesenteric lymph nodes (MLNs), pancreas, liver, spleen and blood. Compared with the control group, both rPAF-AH and BN 52021 decreased BT in the pancreas and blood. In addition, rPAF-AH decreased BT in the MLNs. We also found that PAF receptor antagonists suppressed the elevation in IL-6 levels. CONCLUSION: PAF antagonists attenuated the severity of experimental AP and reduced pancreatitis-induced BT to distant sites.


Asunto(s)
1-Alquil-2-acetilglicerofosfocolina Esterasa/uso terapéutico , Diterpenos/uso terapéutico , Lactonas/uso terapéutico , Pancreatitis/tratamiento farmacológico , Pancreatitis/microbiología , Enfermedad Aguda , Animales , Bacterias/aislamiento & purificación , Ceruletida/toxicidad , Sistema Digestivo/microbiología , Ginkgólidos , Masculino , Pancreatitis/inducido químicamente , Glicoproteínas de Membrana Plaquetaria/antagonistas & inhibidores , Ratas , Ratas Wistar , Receptores Acoplados a Proteínas G/antagonistas & inhibidores , Proteínas Recombinantes/uso terapéutico
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