[Piezoquartz immunosensors for assessing the interactions between Yersinia enterocolitica lipopolysaccharides and antibodies to them].

The utility of a piezoquartz immunosensor coated with lipopolysaccharides (LPS) for the quantification of antibody specificities was demonstrated. Immunochemical reactions were monitored according to the changes in the weight of sensor bioreceptor layer with high sensitivity (detection limit, 1.3 microg/ml) and assay rate (10 min) without any additional labels. The capabilities of this sensor were demonstrated by the example of quantifying the cross-reactivity of blood serum antibodies with the LPS of Yersinia enterocolitica serotypes O:3, O:5, O:5.27, O:6.30, and O:6.31. The proposed approach is promising for clinical diagnostics of yersiniosis, an infectious intestinal disease.

[Inhibition of prokaryote and eukaryote cells adhesion by sea proteobacteria Pseudoalteromonas nigrifaciens KMM 156 lipopolysaccharide and its fragments].

The impact of Pseudoalteromonas nigrifaciens KMM 156 lipopolysaccharide and its fragments on adhesion of prokaryote and eukaryote cells was studied. The lipopolysaccharide and O-specific polysaccharide identical by its structure to capsular polysaccharide lowered the number of the Yersinia pseudotuberculosis bacteria attached to sheep erythrocytes and adhesion of mouse neutrophils to plastic. The kora oligosaccharide had no effect on the processes.

Structure of the repeating unit of the O-specific polysaccharide of the lipopolysaccharide of Yersinia kristensenii strain 490 (O:12,25).

The O-specific polysaccharide isolated by mild acid degradation of the lipopolysaccharide of Y. kristensenii strain 490 (O:12,25) contained D-glucose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, 2-acetamido-2,6-dideoxy-L-galactose, glycerol, and phosphate in the ratios 2:2:1:1:1:1. On the basis of 31P- and 13C-n.m.r. data, methylation analysis, dephosphorylation, solvolysis with anhydrous hydrogen fluoride, and Smith degradation, it was concluded that the repeating unit of the polysaccharide was a branched hexaosylglycerol phosphate with the following structure. [formula: see text]

Structural studies of O-specific polysaccharide chains of the lipopolysaccharide from Yersinia enterocolitica serovar O:10.

Lipopolysaccharide (LPS) was isolated from Yersinia enterocolitica serovars O:10 and O:10 KL and the structural pattern of O-specific sugar chains elucidated. The rhamnan and L-xylulose (L-threo-pent-2-ulose) as constituents of the O-specific polysaccharide were obtained by autohydrolysis of the LPS. The rhamnan was shown to be a linear, alpha-(1-->3)-linked polysaccharide in the D configuration. L-Xylulose was purified using paper chromatography on a preparative scale and its structure was confirmed by 13C NMR spectroscopy. Using sugar and methylation analysis and 13C NMR spectroscopy of the LPS and the rhamnan, the structural features of the disaccharide repeating unit of the Y. enterocolitica O:10 O-specific polysaccharide were elucidated as: [formula: see text]

Structure of a phosphorylated polysaccharide from Shewanella putrefaciens strain S29.

A phosphorylated polysaccharide was isolated from the aqueous layer of the phenol-water extract of a non-halophilic bacterium Shewanella putrefaciens strain S29. The glycosyl phosphate linkage in the polysaccharide was split under mild acid conditions to give, after borohydride reduction, a phosphorylated oligosaccharide-alditol. On the basis of sugar analysis and 1H, 13C and 31P NMR spectroscopy, including 2D COSY, relayed COSY, rotating-frame NOE spectroscopy (ROESY), heteronuclear 13C,1H COSY, and H-detected heteronuclear 1H,31P multiple-quantum coherence (HMQC), it was concluded that the polysaccharide is built up of tetrasaccharide-phosphate repeating units having the following structure: [sequence: see text] where QuiNAc and Qui4NAc are 2-acetamido-2,6-dideoxyglucose and 4-acetamido-4,6-dideoxyglucose, respectively.

Structure of the capsular polysaccharide from Alteromonas sp. CMM 155.

Capsular polysaccharide (CPS) was obtained by water-saline extraction of the Alteromonas sp. CMM 155. On the basis of solvolysis with anhydrous HF and 1H- and 13C-NMR spectral data, including NOE experiments, it was concluded that the capsular polysaccharide had the following structure containing novel N-acyl-amino sugar and bacillosamine residues: --> 3)-alpha-D-GalpNAc-(1 --> 4)-alpha-L-GalApNAc(1 --> 3)- alpha-D-QuipNAc4NAc-(1 --> 3)-beta-D-Quip4NAlaAc-(1 -->

Structure of the O-specific polysaccharide of the lipopolysaccharide from Yersinia kristensenii O:25.35.

Mild hydrolysis of the lipopolysaccharide (LPS) from Yersinia kristensenii serovar O:25.35 with acid afforded the O-specific polysaccharide (PS) which contained D-glucose, D-galactose, 2-acetamido-2,6-dideoxy-L-galactose, 2-acetamido-2-deoxy-D-glucose, glycerol, and phosphate in the ratios 3:1:1:1:1. On the basis of 31P and 13C NMR spectroscopy, hydrolysis, methylation studies, Smith degradation, and dephosphorylation, the repeating unit of PS was shown to have the following structure. [formula: see text]

Studies of lipopolysaccharides from Yersinia pseudotuberculosis subtypes VA and VB.

Lipopolysaccharides (LPS) from varions strains of Yersinia pseudotuberculosis type V have been isolated and characterised. Differences in sugar composition and serological activity of LPS from various strains within the same subtype of Y. pseudotuberculosis have been revealed.

Structure of the O-specific polysaccharide from Pseudoalteromonas elyakovii sp. nov. CMM 162.

The structure of the O-specific polysaccharide from lipopolysaccharide of Pseudoalteromonas elyakovii sp. nov. CMM 162 on the basis of NMR data, Smith degradation and methylation study was elucidated as follows:-->2)-alpha-D-Glcp-(1-->4)-beta-D-GalpNAc-(1--> 3)-alpha-D-Galp-(1-->3)-beta-D-Galp-NAc-(1-->6)-alpha-D-Glcp-(1-->.

Structure of the acidic polysaccharide chain of the lipopolysaccharide of Shewanella alga 48055.

A lipopolysaccharide (LPS) with an acidic polysaccharide chain was isolated from the bacterium Shewanella alga strain 48055 and cleaved selectively at the glycosidic linkage of N-acetylneuraminic acid to give a tetrasaccharide. Studies of the tetrasaccharide and the O-deacylated LPS by 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, rotating-frame NOE spectroscopy (ROESY), and H-detected 1H, 13C heteronuclear multiple-quantum coherence (HMQC) experiments, revealed the following structure of the polysaccharide repeating unit: -->3)-beta-D-GalpA6GroN-(1-->3)-beta-D-GlcpNAc-(1-->3)-alpha-D- GalpA6GroN- (1-->4)-alpha-Neup5Ac-(2--> where GroN is an amidically linked residue of 2-amino-1,3-propanediol (2-amino-2-deoxyglycerol). A similar structure, but with 2-acetamido-2,6-dideoxy-D-glucose instead of 2-acetamido-2-deoxy-D-glucose, has been reported previously for the polysaccharide chain of a non-O1 Vibrio cholerae H11 LPS [E. V. Vinogradov, O. Holst, J.E. Thomas-Oates, K.W. Broady, and H. Brade, Eur. J. Biochem., 210 (1992) 491-498].

Structure of the capsular polysaccharide from Alteromonas nigrifaciens IAM 13010T containing 2-acetamido-2,6-dideoxy-L-talose and 3-deoxy-D-manno-octulosonic acid.

A capsular polysaccharide was obtained from Alteromonas nigrifaciens IAM 13010T by saline extraction. On the basis of 1H and 13C NMR spectroscopy, including one-dimensional (1D) NOE spectroscopy, 2D rotating-frame NOE spectroscopy (ROESY), and 1H-detected heteronuclear 1H,13C multiple-quantum coherence (HMQC), it was concluded that the polysaccharide contained inter alia an acidic sugar, 3-deoxy-D-manno-octulosonic acid (Kdo), and a rare amino sugar, 2-acetamido-2,6-dideoxy-L-talose (L-6dTalNAc, N-acetylpneumosamine), and has a pentasaccharide repeating unit of the following structure: [equation: see text]

Structure of a colitose-containing O-specific polysaccharide of the marine bacterium Pseudoalteromonas tetraodonis IAM 14160(T).

O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Pseudoalteromonas tetraodonis type strain IAM 14160(T) and studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, 1H,(13)C HMQC and HMBC experiments. The polysaccharide was found to consist of hexasaccharide repeating units containing one residue each of D-Gal, D-GlcA, D-GalNAc and D-GlcNAc and two residues of 3,6-dideoxy-L-xylo-hexose (colitose, Col) and having the following structure:In common with the polysaccharides of some other bacteria, the polysaccharide studied contains a tetrasaccharide fragment alpha-Colp-(1-->2)-beta-D-Galp-(1-->3)-[alpha-Colp-(1-->4)]-beta-D-GlcpNAc, which is a colitose ('3-deoxy-L-fucose') analogue of the Lewis(b) blood group antigenic determinant.

[Structure of the O-specific polysaccharide chain of the lipopolysaccharide from Yersinia intermedia serotype O:4.33]. / Struktura O-spetsificheskoi polisakharidnoi tsepi lipopolisakharida Yersinia intermedia serovara O: 4,33.

O-specific polysaccharide has been isolated on autohydrolysis of lipopolysaccharide from Yersinia intermedia O: 4.33 (strain 1476) and shown to consist of the yersiniose B (3.6-dideoxy-4-C-(1-hydroxyethyl)-xylo-hexose) and 2-acetamido-2-deoxy-D-galactose residues in a molar ratio of 1 : 2. Acid hydrolysis, methylation. solvolysis with anhydrous hydrogen fluoride. and 13C-NMR studies indicate the polysaccharide to be composed of trisaccharide repeating units of the following structure: (Formula: see text).

[Structure of O-specific polysaccharide chains of the Yersinia bercoviery O:10 lipopolysaccharide]. / Struktura O-spetsificheskikh polisakharidnykh tsepei lipopolisakharida Yersinia bercoviery 0:10.

O-specific polysaccharide composed of D-rhamnose and 3,6-dideoxy-4-C-(L- glycero-1-hydroxyethyl)-D-xylo-hexopyranose (yersiniose A) residues was obtained on mild acid degradation of the Yersinia bercovieri lipopolysaccharide. On the basis of 1H- and 13C-NMR data, methylation studies and Smith degradation, the following structure was suggested for the polysaccharide repeating unit: [formula: see text]

[Structure of the O-specific polysaccharide chain of the Yersinia pseudotuberculosis lipopolysaccharide (serovar II C)]. / Struktura O-spetsificheskoi polisakharidnoi tsepi lipopolisakharida Yersinia pseudotuberculosis (serovar IIC).

An O-specific polysaccharide has been isolated on mild acid hydrolysis of lipopolysaccharide from Yersinia pseudotuberculosis serovar IIc and shown to consist of abequose, D-mannose and 2-acetamido-2-deoxy-D-galactose residues in the ratio 0.8:3:1. From the results of acid hydrolysis, 13C NMR, methylation and periodate oxidation studies the structure of the repeating unit of the O-specific polysaccharide is deduced as follows: (formula; see text)

[Structure of the O-specific polysaccharide chain of Yersinia aldovae lipopolysaccharide]. / Struktura O-spetsificheskoi polisakharidnoi tsepi lipopolisakharida Yersinia aldovae.

O-specific polysaccharide has been isolated on mild hydrolysis of lipopolysaccharide from Yersinia aldovae and shown to consist of 2-acetamido-2-deoxy-D-glucose, D-glucose, 2-acetamido-2-deoxy-D-galactose, and 3,6-dideoxy-3- [(R)-3-hydroxybutyramido]-D-galactose in molar ratio 2:2:1:1. Acid hydrolysis, methylation, solvolysis with anhydrous hydrogen fluoride, 1H and 13C NMR studies indicated the polysaccharide to be composed of hexasaccharide repeating units of the following structure: [formula see text].

[The structure of O-specific polysaccharide isolated from the lipopolysaccharide of Yersinia intermedia strain 180]. / Struktura O-spetsificheskogo polisakharida, vydelennogo iz lipopolisakharida Yersinia intermedia shtamma 680.

An O-specific polysaccharide from lipopolysaccharide of Yersinia intermedia pathogenic strain 680 has been isolated and shown to be a serologically active fructane. Serological specificity of the lipopolysaccharide and the fructane was studied by reactions of precipitation and of inhibition of passive hemolysis. On the basis of methylation studies, 13C NMR spectroscopy, and immunochemical data the following structure was proposed for the repeating unit of the O-specific polysaccharide: (Formula: see text)

[A new branched-chain monosaccharide from the Yersinia enterocolitica serotype O:4.32 lipopolysaccharide]. / Novyi razvetvlennyi monosakharid iz lipopolisakharida Yersinia enterocolitica serovara O:4,32.

A component of the Yersinia enterocolitica O:4,32 lipopolysaccharide has been shown to be a second representative of the new class of monosaccharides and possess the structure of 3,6-dideoxy-4C-(1-hydroxyethyl)-D-xylo-hexose (yersiniose).

[Structure of the repeating chain of the O-specific polysaccharide of Vibrio fluvialis]. / Struktura povtoriaiushchegosia zvena O-spetsificheskogo polisakharida Vibrio fluvialis.

O-Specific polysaccharide chain of the Vibrio fluvialis lipopolysaccharide is built up of pentasaccharide repeating units, containing one N-acetyl-D-glucosamine and four L-rhamnose residues. The structure of the polysaccharide was elucidated using two-dimensional correlation 1H-NMR-spectroscopy, 13C-NMR-spectroscopy and nuclear Overhauser effect and confirmed by methylation analysis and selective cleavage of N-acetylglucosamine residues by the N-deacetylation-deamination method which yielded linear L-rhamnan representing the backbone of the polysaccharide. Thus, the repeating unit of the O-specific polysaccharide has the following structure: (formula; see text)

[The structure of the O-specific polysaccharide chain of the lipopolysaccharide of Yersinia pseudotuberculosis serovar VII]. / Stroenie O-spetsificheskoi polisakharidnoi tsepi lipopolisakharida Yersinia pseudotuberculosis serovara VII.

An O-specific polysaccharide of Yersinia pseudotuberculosis serovar VII has been isolated and characterized. The polysaccharide consists of colitose, D-glucose and 2-acetamido-2-deoxy-D-galactose in the ratio 1 : 2 : 2. From the results of methylation analysis, partial acid hydrolysis, 1H and 13C NMR spectroscopy the structure of the repeating unit of the O-specific polysaccharide is deduced as follows: