Regional distribution of acid mucopolysaccharides in the kidney.

Kidneys from 20 dogs were dissected into cortical and medullary components and analysed for acid mucopolysaccharide content. Heparitin sulfate accounted for approximately 80% of cortical acid mucopolysaccharide, 10% was chondroitin sulfate B, and 10% was low molecular weight hyaluronic acid. Medullary tissue exhibited a 4- to 5-fold higher concentration of acid mucopolysaccharide than did cortical tissue, and the dominant compound was moderately highly polymerized hyaluronic acid. While chondroitin sulfates A and (or) C were not detected in this study, the presence of minor amounts of these substances could not be excluded. A model experiment indicated that hyaluronic acid retards sodium diffusion, apparently due to its viscous properties rather than its electronegativity.

Effect of tridiphane on reproductive parameters in Fischer 344 rats.

Reproductive parameters in Fischer 344 rats were evaluated following dietary administration of tridiphane for 2 successive generations. Male and female rats in the fO generation were maintained on test diets formulated to provide 0, 1, 5, or 30 mg tridiphane/kg per day for 104 days starting at 6 weeks of age. The rats were allowed to mate twice to produce the f1a and f1b litters. Offspring from the f1b litters were maintained on test diets for 125 days and then mated to produce the f2a and f2b litters. Reproductive parameters including fertility, litter size and growth and survival of the pups were monitored. Selected weanlings were examined for gross and histologic lesions. Treatment related effects in the f0 adults were limited to the 30 mg/kg dose group and consisted of increases in relative liver weights in both sexes and depressed body weights in females. Liver weight relative to body weight was elevated in weanlings of both sexes in the f1a (30 mg/kg) litters but only in male offspring in the f1b litters. In the f1 adult females from the 30 mg/kg per day group, body weights were depressed throughout the 125-day treatment period and during lactation of both the f2a and the f2b litters. In the second generation, only male weanlings from the f2a litters, 5 mg/kg per day group had elevated (relative) liver weights. No consistent adverse effects on reproduction, fertility, or neonatal growth or survival were observed in either the f1 or f2 generations, and gross and histological examination of livers from weanlings did not reveal any treatment related lesions.

Taste acuity and zinc status in chronic renal disease.

The malnutrition often associated with progressive renal failure may be related to a progressive deterioration of taste acuity. To determine the relationships between taste acuity, zinc status, and progressive renal failure, 27 subjects were divided into three groups and classified as having mild, moderate, or severe renal failure, with creatinine clearance of 41 to 75, 15 to 40, and 5 to 14 ml/minute, respectively. The subjects were evaluated for recognition taste thresholds for sour and sweet by the Cornsweet method. Serum, urinary, and salivary levels for zinc were determined by atomic absorption techniques. Food diaries were kept for two days; intakes of protein and zinc were calculated from the diaries. There was a significant (p less than .01) inverse relationship between creatinine clearance and recognition taste thresholds for sour and sweet. Serum zinc levels were significantly lower (p less than .01) for subjects with severe renal failure than for subjects with mild renal failure. The subjects who ingested more protein also ingested more zinc.

Teratological evaluation of picloram potassium salt in rabbits.

The embryotoxic and teratogenic potential of orally administered picloram potassium salt was evaluated in New Zealand white rabbits. Artificially inseminated rabbits were given 0, 40, 200 or 400 mg picloram acid equivalent/kg body weight/day in the form of picloram potassium salt in aqueous solution on days 6 to 18 of gestation. The foetuses were removed for examination on day 29 of gestation. Transient weight loss was observed among rabbits given 200 or 400 mg/kg/day of the test material, though the total weight gain of the treated groups during gestation was comparable to that of controls. A few isolated, sporadic cases of foetal malformations were observed in the dosed groups, but there was no indication of a dose-related embryotoxic or teratogenic response to treatment.

Effect of shock strength on survival and acute cardiac damage induced by open-thorax defibrillation of dogs.

The safety of open thorax defibrillation with single damped sine-wave shocks and 6-cm-diameter electrodes was evaluated in healthy anesthetized dogs. Twenty-one dogs were allotted to 6 groups: Group A were nonshocked controls and groups B through F were given single shocks of 4-, 7-, 12-, 19-, or 32-fold, respectively, greater than a defibrillation threshold dose (30 mA/g of heart). Immediate postshock death resulted in group F dogs; group A through E dogs survived and were killed after 2 days. The incidence and severity of cardiac morphologic damage increased with shock strength (mild damage occurred in 1 of 3 dogs in group C and in 3 of 4 dogs in group D and severe damage occurred in 2 of 3 dogs in group E). The cardiac lesions were characterized grossly and microscopically. In dogs that died immediately after shocking, damage was apparent as pale circular zones of edema and myofibrillar degeneration in the ventricular free walls beneath the electrode placement sites on the cardiac surface. In the dogs that survived 2 days, the defibrillator-induced areas of myocardial necrosis and calcification were concentrated in arc or ringlike patterns beneath the periphery of the electrode placement sites. All dogs that were studied 2 days after shocking had mild fibrinous pericarditis. Postshock electrocardiographic changes were not good indicators of cardiac damage because the mild epicardial inflammatory reaction associated with the surgical procedure produced large ST and T wave changes which masked any changes associated with myocardial necrosis induced by the electric shocks. It was concluded that a substantial safety margin exists between the required defibrillation threshold shock dose and the large shocks required to produce marked cardiac damage or death in healthy dogs.

Revolutionary advances in organic foods.

'Organic' is a labelling term that denotes products produced under the authority of the Organic Foods Production Act. Before a product can be labelled 'organic', a government-approved certifier inspects the farm where the food is grown to make sure the farmer is following all the rules necessary to meet the US Department of Agriculture (USDA) organic standards. Companies that handle or process organic food before it gets to your local supermarket or restaurant must be certified, too. Along with the national organic standards, the USDA developed strict labelling rules to help consumers know the exact content of the food they buy. It is important to emphasise that the USDA has not made any health claims for organic foods. It is indeed fortunate that the US Department of Health and Human Services, Centers for Disease Control and Prevention, USDA and the Environmental Protection Agency are now expanding their research to explore the scientific basis for the health benefits of organic foods.

Dementia: physician approaches to the caregiver's problems.

The demented patient requires medical and often psychiatric supervision, but the caregiver is often neglected. This article identifies special issues for caregivers that may need attention from the demented patient's physician. The physician's attention can benefit both the patient and the caregiver and save the physician valuable time.

Group psychotherapy for patients with dementia.

Some of the goals of our group psychotherapy sessions on the inpatient unit include (1) creation of an emotional climate of acceptance and warmth that helps patients learn to accept themselves and their feelings, (2) frequent intervention by the group facilitator/therapist to help facilitate social interaction for patients whose communication ability is impaired, (3) opportunity for patients to experience the feeling of belonging, of being part of a group, (4) opportunity for patients to ventilate feelings and rediscover mutual kinds of experience, (5) opportunity for patients to reminisce about past accomplishments and give new meaning to their current lives, and (6) creation of a platform for patients to achieve a sense of self by expressing personal opinions in an environment of respect and acceptance. The outcome of group therapy for demented as well as nondemented patients should be increased ability to cope with losses at several levels, promotion of new skills, increased adaptation skills, and increased ability to accept change. We also want patients to learn to express feelings and to realize that the expression of feelings can have a positive outcome (relief from repression, clarification of ambivalence, solutions, etc).

Photoinduced refractive-index changes in two-mode, elliptical-core fibers: sensing applications.

Photoinduced refractive-index changes in two-mode, elliptical-core optical fibers are shown to affect the differential phase modulation between the LP(01) and the LP(11)(even) modes. This change in beat length is dependent on the amount of strain induced in the fiber while the grating is being formed. We present experimental results that agree with conventional coupled-mode theory and propose the use of such sensors for weighted and distributed applications.