Suprabasal p53 immunoexpression is strongly associated with high grade dysplasia and risk for malignant transformation in potentially malignant oral lesions from Northern Ireland.

AIMS: No good predictive marker for the malignant transformation of potentially malignant oral lesions (PMOLs) is currently available. This study re-evaluated the value of p53 immunoexpression to predict malignant transformation of PMOLs after discounting possible confounding factors. METHODS: PMOLs from 18 patients who showed progression to carcinoma, 16 of the respective carcinomas, and PMOLs from 18 matched controls were evaluated by immunohistochemistry (IHC) for p53 expression. A mouse monoclonal antibody that detects wild-type and mutant forms of human p53 was used. The p53 immunostaining pattern was also correlated with the degree of dysplasia. RESULTS: Suprabasal p53 staining was significantly associated with high grades of dysplasia (p < 0.01). The specificity and positive predictive value (PPV) for malignant transformation of suprabasal p53 staining were superior to the assessment of dysplasia, but sensitivity was inferior. All carcinomas derived from PMOLs with suprabasal p53 showed strong p53 immunostaining. However, the absence of suprabasal p53 staining and/or dysplastic changes did not preclude malignant transformation in a considerable proportion of PMOLs. CONCLUSIONS: This study confirms and extends previous findings that suprabasal p53 immunoexpression has a high PPV for malignant transformation of PMOLs and can be used as a specific marker for lesions that are at high risk for malignant transformation. The absence of suprabasal p53 staining (that is, absence of, or basal, p53 staining) is non-informative for prognostic purposes. Because of its limited sensitivity, p53 IHC is not a substitute for the assessment of dysplasia in the evaluation of PMOLs. Instead, p53 IHC emerges as a clinically useful supplement of histopathological assessment in the prognosis of PMOLs.

Trends in the relative frequency of histologically diagnosed epithelial dysplasia and intra-oral carcinoma in Northern Ireland, 1975-1989.

Trends in the incidence of histologically diagnosed dysplastic lesions of the intra-oral mucosa have been investigated for the period 1975-89 in a well defined population of 1.5 million. These have been contrasted and compared with trends in the incidence of intra-oral carcinoma. Cases were ascertained from the records of all the histopathology laboratories that serve the Northern Ireland population. Over the 15-year period, there were 135 cases of histologically diagnosed epithelial dysplasia. In contrast to the significant increase in the incidence of intra-oral carcinoma, there was no significant change in the annual age standardised incidence of dysplastic lesions over the period. The ratio of malignant to dysplastic diagnoses rose from 2.5:1 to 5.4:1. Only 24 of the dysplastic lesions were known to have subsequently progressed to malignant carcinoma, representing 4.5% of all invasive tumours diagnosed during 1975-89. The results highlight a number of unresolved issues regarding the natural history of intra-oral carcinoma.

Trends in the incidence of histologically diagnosed intra-oral squamous cell carcinoma in Northern Ireland, 1975-89.

Studies in Britain point to a rise in the incidence of intra-oral cancer in the last 20 years, paralleling trends evident in other European countries. Cases of histologically-diagnosed primary intra-oral squamous cell carcinoma have been ascertained by reviewing the records of pathology departments in Northern Ireland. Trends in the incidence of the disease have been determined for the period 1975-89. There has been a significant increase in the incidence among men, rising from 1.78 to 3.14 per 100,000. In women over the same period the incidence rose from 0.87 to 1.19 per 100,000, but this change was not significant. These trends concur with recent findings from other countries.

Prevention and detection of oral cancer: the views of primary care dentists in Northern Ireland.

Our objective was to describe the management of oral cancer and pre-cancer as stated by primary care dentists and their views on screening. We conducted a survey of all general dental practitioners and community dentists in Northern Ireland (n = 635), to which 428 replied (response rate: 67%). 94% stated that examination of the oral soft tissues constituted part of their usual practice during the regular dental check-up. Suspicious lesions were generally referred early, 68.5% of dentists referring white lesions within one month of presentation. The corresponding figures for red lesions, lumps and persistent ulcers were 80.1%, 89.7% and 91.7%. The incidence of oral cancer was over-estimated (median '70' cases/year, versus the true figure of approximately 40/year) as, in all likelihood, was the percentage by which mortality could be reduced by screening (median: 50%). Accordingly the adoption of a screening programme was favoured over investment in health promotion. Indeed, only 14% said that their patients records routinely contained information about smoking or alcohol habits. Although there are some areas of practice which could improve and the potential of screening is probably over-valued, primary care dentists in Northern Ireland already opportunistically screen and refer patients promptly.

Variations in the presenting and treatment features in reimplanted permanent incisors in children and their effect on the prevalence of root resorption.

OBJECTIVE: To examine variations in the presentation and treatment of reimplanted incisors in children and to determine the effect of these on the prevalence of external root resorption. SETTING: Departments of Paediatric Dentistry, Belfast and Newcastle upon Tyne. DESIGN: Recording of the timing of the injury and the storage mediums (including air) and of reimplantation, the stage of root development, the degree of contamination and the time of commencement of root treatment. Cases were reviewed clinically and radiographically at intervals of 3 months. Root resorption was classified as present or absent. Logistic regression and cross-tabulations were produced with the presence of resorption set as the outcome. RESULTS: 128 reimplanted permanent incisor teeth, their median dry time prior to reimplantation being 15 minutes (range 4-52 mins), the median time in a liquid medium being 45 minutes (range 0-650 mins), with a median splinting time of 15 days (range 4-52 days) and a median pulp extirpation time of 15 days (range 0-612 days). There was a lower prevalence of resorption when the period of dryness was less than or equal to 5 minutes (p = 0.025). The prevalence of resorption in teeth with no visible contamination was 57.1%, for those with contamination which were washed clean it was 75%, in those rubbed clean it was 87.5%, and it was 100% for those reimplanted with visible contamination still present (p = 0.014). The corrected odds ratio for contamination was 2.99 and for an extension of 10 minutes of dryness it was 1.29. CONCLUSION: The degree of contamination and the period of dryness were the major risk factors for resorption in this study of reimplanted teeth in children.

Outcomes for root-fractured permanent incisors: a retrospective study.

PURPOSE: The objective of this study was to assess the outcomes for treated root-fractured permanent incisors with respect to pulp vitality, root tissue union, and tooth survival and to examine the effects of clinical and radiographic parameters and rigid splinting on the outcome. METHODS: Eighty-four teeth were identified and data extracted from case notes prior to transfer to an SPSS data base for analysis. The odds ratios for each factor were calculated and the significance of differences was determined. Tooth loss and relevant risk variables were examined using Cox's regression model and Kaplan-Meyer survival curves. RESULTS: Fourteen (17%) had fractures in the apical third, 47 (56%) in the middle third, and 23 (27%) in the coronal (gingival) third. Twenty-four (29%) also had crown fractures involving enamel and dentine. Crown fractures were identified as significant risk factors for pulp vitality. Loss of pulp vitality, horizontal displacement, and extrusive displacement of the coronal fragment were significant risk factors for hard root tissue union. Survival was poorest with gingival third fractures with 14 (61%) of these teeth being lost. Splinting rigidly had no significant effect on pulp vitality and type of root tissue healing. CONCLUSIONS: Loss of pulp vitality was significantly associated with enamel-dentine crown fracture. Hard root tissue union was significantly affected by pulp necrosis and luxation of the coronal fragment. Survival was poorest for root fractures within the gingival third of the root. Splinting with rigid fixation had no significant effect on pulp vitality and type of root tissue union.

Recent advances in the management of patients with haemophilia and other bleeding disorders.

Much attention has been focused recently on barriers to dental care, particularly for patients with disabilities, from the perspective of the patient and the dental team. Treatment of patients with haemorrhagic disorders may cause a certain amount of anxiety in dental staff. The authors of this paper aim to reduce this anxiety by clarifying confusion between the haemorrhagic disorders and making recommendations concerning the dental care of such patients.

Birth prevalence of cleft lip and palate in Northern Ireland (1981 to 2000).

OBJECTIVE: The prevalence of cleft lip and/or palate (CL/P) in Northern Ireland (NI) was last reported for 1980 through 1990. This study was undertaken to update the prevalence of CL/P in NI for the 20-year period 1981 to 2000, to determine the pattern of prevalence, and to report the proportion of different cleft types and sex distribution. DESIGN: Retrospective, population-based analysis. PATIENTS/PARTICIPANTS: All live born children with CL/P in NI from 1981 to 2000 were included. A total of 750 cases were identified. Resident births outside NI, stillbirths, abortuses, and children born with atypical orofacial clefts were excluded. Those with syndromes and submucous clefts were included in the study. RESULTS: The overall prevalence of children born with CL/P within NI for the period 1981 to 2000 was 1.47 per 1000 live births, or 1:682. This was consistent with the findings reported by other U.K. studies. There were no significant changes in the prevalence rates over any 5-year period. No significant seasonality trends were noted. Clefts of the palate only were always in the majority. More boys than girls were affected by cleft lip with or without cleft palate. There was a significant left-sided predilection for unilateral clefting of the lip. CONCLUSIONS: There have been no significant changes in the birth prevalence of children born with CL/P or the distribution or laterality of cleft type in the NI population during the past 20 years.

Freeman-Sheldon syndrome: a case report.

BACKGROUND: Freeman-Sheldon Syndrome is a rare craniofacial syndrome that has not been described in the dental literature to date. The main feature of relevance is severe microstomia, which limits access for routine dental care. CASE REPORT: Dental treatment was carried out successfully under local anaesthetic for a young child with Freeman-Sheldon syndrome. A novel coloured compomer material was helpful in the management of the case. CONCLUSION: The importance of early referral of children with rare craniofacial anomalies to Specialist Paediatric Dental services is highlighted.

The effect of the position and orientation of unerupted premaxillary supernumerary teeth on eruption and displacement of permanent incisors.

The hospital records of 101 consecutive patients admitted for removal of premaxillary supernumerary teeth were examined. From the clinical notes and preoperative anterior occlusal radiographs the following information was noted: the position and orientation of the supernumerary teeth, and whether they caused delayed eruption, rotation or displacement of the associated permanent incisors. There were 140 supernumerary teeth (mean per child = 1.39). Delayed eruption of permanent incisors occurred in 56% of cases. Supernumerary teeth situated between central incisors caused eruption delay infrequently, but were associated with rotation and displacement of permanent incisors more frequently than those that overlapped the incisors on the radiographs. Supernumerary teeth that were orientated vertically caused eruption delay more frequently than those that were inverted, but the orientation of supernumerary teeth did not influence rotation or horizontal displacement of the permanent incisors.

Candida infection of a persistent palatal ulcer in a 20-month-old child.

A 20 month-old infant presented with a painless, persistent lesion on the hard palate which had been present for 6 months. The lesion was aggressive in appearance, approximately 2 cm in diameter with raised, rolled edges, and there was what appeared to be granulation tissue present together with some necrotic slough in its base. It was considered to represent a traumatized area with superimposed secondary Candida infection. A direct smear of the lesion was positive for Candida. Antifungal therapy, and an attempt to avoid further irritation by advising the mother not to give the child a feeding bottle whenever possible, resulted in complete healing of the lesion. There has been no recurrence in 2 years.

A computer software package to facilitate clinical audit of outpatient paediatric dentistry.

This paper reports the development and function of a computer program for clinical audit of outpatients in hospital paediatric dentistry in the United Kingdom. The project was one of several national audit projects supported by the Faculty of Dental Surgery, Royal College of Surgeons of England, and funded by the Department of Health. The software is designed to record data utilizing a nationally-agreed minimum set of codes for dental diagnosis, treatment and medical complications, together with patient demographic details pertaining to source of referral, age and sex of patient, and area of residence. A significant component of the software is a flexible report generator which allows data to be analysed using any combination of the above criteria. Audit of activity in hospital dentistry is desirable to enable comparisons to be made between groups of patients and treatments related to diagnosis. This provides the information necessary to enable improvements in clinical practice whilst also assisting the development of both local and national clinical guidelines. Use of this software package will enable such comparisons to be made not only within one hospital but also between different hospital and regions in the United Kingdom.

A prospective study of factors affecting survival of replanted permanent incisors in children.

OBJECTIVE: To examine the factors associated with the onset of resorption in replanted avulsed permanent incisor teeth and to demonstrate their patterns of survival free of resorption. SETTING: Department of Paediatric Dentistry, Belfast. DESIGN: Prospective recording of the timing of the injury and of replantation, the storage media, the degree of root development and contamination, and the completion of root treatment. Clinical and radiographic reviews at 3-month intervals. Root resorption classified as replacement and inflammatory. Logistic regression and survival curves with freedom from resorption as outcomes. RESULTS: In 50 avulsed incisors in children aged 6-16 years the best predictor of overall resorption was total time of dryness, and that for replacement resorption was total extra-oral time. The timing of detection of resorption varied from 102 days to 997 days. CONCLUSION: Both total extra-oral time and time stored dry are important factors for the onset of resorption in replanted avulsed teeth in children.

Analysis of an emergency dental service provided at a children's hospital.

An analysis was undertaken of the evening, weekend and public-holiday emergency dental service provided at the Royal Belfast Hospital for Sick Children during one calender year (1987). Dental emergencies accounted for 4% of all attendances during these periods at the Accident & Emergency Department. Toothache, with or without abscess, was the most frequent dental complaint (49%) and abscesses were most frequently associated with primary first molars. Acute trauma was the second most frequent dental complaint (39%) and traumatic injuries were most commonly caused by falls and bicycle accidents. Tooth displacement was was the most common dental injury of primary and permanent teeth. Complaints other than toothache, abscess or traumatic injury accounted for only 12% of dental emergencies.

A comparative study of the gingival and lateral periodontal cysts.

The object of this study is to separate the gingival and the lateral periodontal cysts of adults using both clinical and histological features. It involved an investigation of 2 cases from each category. The distinguishing clinical feature was the ability to determine the involvement of the periodontal ligament at surgical exploration. The histological feature of note was the presence of plaque-like lining elevations in the lateral periodontal cyst. These appeared to be due to an oedematous change in the true intra-bony cyst lining, perhaps occurring during the process of cyst expansion.