RESUMEN
The purpose of this study was to determine the antimetastatic potential of lonazolac. Intravenous inoculation of Lewis lung carcinoma (LLC) or melanoma B-16 (B-16) cells induced macroscopically detectable lung metastases on day 15 after inoculation. After pre- and post-treatment with lonazolac-Ca at oral doses of 1, 25, and 50 mg/kg, the numbers of animals with lung metastases and the score of metastases significantly decreased. Lonazolac-Ca had similar effects also on the formation of spontaneous lung metastases. After treatment with lonazolac-Ca at oral daily doses of 1, 25, and 50 mg/kg during 8 days (LLC) or 10 days (B-16) after inoculation of the tumor cells, the number of animals developing spontaneous lung metastases and the score of metastases also decreased. Intravenous injection of lonazolac natrium was effective in protecting the mice inoculated with 1 X 10(6) melanoma B-16 cells against acute pulmonary embolic death.
Asunto(s)
Antineoplásicos , Neoplasias Pulmonares/secundario , Melanoma/secundario , Células Neoplásicas Circulantes/efectos de los fármacos , Pirazoles/farmacología , Animales , Neoplasias Pulmonares/prevención & control , Masculino , Melanoma/prevención & control , Ratones , Ratones Endogámicos C57BL , Metástasis de la Neoplasia , Embolia Pulmonar/prevención & controlRESUMEN
Alpha-(4-cyclohexylphenyl)propionic acid, a potent inhibitor of platelet aggregation, has appeared to reduce the quantity and size of Lewis lung carcinoma (LLC) tumor nodules in the lungs. The number of animals developing the tumor colonies in the lungs was decreased by 34% to 49% either when the tumor cells were injected i.v. and alpha-(4-cyclohexylphenyl)propionic acid was administered during 3 days, or when the tumor cells were transplanted i.m. The primary tumor was removed 6 days later and the drug administered during 8 or 21 days.
Asunto(s)
Antineoplásicos , Neoplasias Pulmonares/tratamiento farmacológico , Fenilpropionatos/uso terapéutico , Animales , Línea Celular , Evaluación Preclínica de Medicamentos , Miembro Posterior , Neoplasias Pulmonares/prevención & control , Neoplasias Pulmonares/secundario , Masculino , Ratones , Ratones Endogámicos C57BL , Trasplante de Neoplasias , Cola (estructura animal)/irrigación sanguíneaRESUMEN
In the present study, we have examined the antimetastatic effect of flurbiprofen, a nonsteroidal antiinflammatory drug, on spontaneous metastases formation in mice bearing Lewis lung carcinoma and these results were compared with effects of other inhibitors of platelet function (mopidamole, dipyridamole, pentoxifylline). Flurbiprofen decreased significantly spontaneous metastases formation in a dose-depending manner. From the results obtained, it appears that the treatment with flurbiprofen, either on days 1-8 or days 1-21, was similarly effective. In mopidamole treated groups the number of animals with lung metastases was significantly decreased. This effect was not observed after treatment with dipyridamole or pentoxifylline.
Asunto(s)
Antineoplásicos , Dipiridamol/uso terapéutico , Flurbiprofeno/uso terapéutico , Neoplasias Pulmonares/tratamiento farmacológico , Mopidamol/uso terapéutico , Pentoxifilina/uso terapéutico , Propionatos/uso terapéutico , Pirimidinas/uso terapéutico , Teobromina/análogos & derivados , Animales , Neoplasias Pulmonares/patología , Masculino , Ratones , Ratones Endogámicos C57BL , Metástasis de la Neoplasia , Agregación Plaquetaria/efectos de los fármacosRESUMEN
The cancerostatic effect of 6-purinyl-N-(2-chloroethyl)thiocarbamate (Cloturin VUFB, VUFB-15686) was studied in detail in mice and rats bearing transplantable tumors. The cytotoxic activities were measured by determining the incorporation rate of 5-iodo-2'-deoxy[6-3H]uridine and uniformly labeled [U-14C]amino acid mixture into trichloroacetic acid-insoluble fraction of Yoshida ascites reticulosarcoma cells during a short-term incubation with the drug. From the results obtained it follows that the new substance is therapeutically more effective in comparison with 6-mercaptopurine (NSC-755). A therapeutic synergism of Cloturin with cytosine arabinoside (NSC-63978) was observed.
Asunto(s)
Antineoplásicos/uso terapéutico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Citarabina/uso terapéutico , Mercaptopurina/análogos & derivados , Compuestos de Mostaza/uso terapéutico , Neoplasias Experimentales/tratamiento farmacológico , Animales , Citarabina/administración & dosificación , ADN de Neoplasias/biosíntesis , ADN de Neoplasias/efectos de los fármacos , Femenino , Linfoma de Células B Grandes Difuso/tratamiento farmacológico , Linfoma de Células B Grandes Difuso/metabolismo , Melanoma Experimental/tratamiento farmacológico , Mercaptopurina/administración & dosificación , Mercaptopurina/uso terapéutico , Ratones , Ratones Endogámicos C57BL , Compuestos de Mostaza/administración & dosificación , ARN Neoplásico/biosíntesis , Ratas , Ratas EndogámicasRESUMEN
Nonsteroid antiinflammatory agents (NSAIA's) (inhibitors of cyclooxygenase and lipoxygenase) of several structural series inhibited growth of Trichoderma viride. The most potent growth inhibitors were indomethacin and its derivative repanidal which inhibited in the range of 0.1 mmol/L. The weakest inhibitors were acetylsalicylic acid and lysine salicylate which exerted only a weak effect at concentrations above 1 mmol/L. The inhibition of growth was accompanied by a stimulation of conidiation in the dark. A light pulse increased the efficiency of these drugs to stimulate the conidiation. Saccharomyces cerevisiae was as sensitive to NSAIA's as T. viride while Botrytis cinerea was less sensitive. The results indicate that arachidonate metabolism may play a role in the growth of fungi and may participate also in the process of conidiation.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Trichoderma/efectos de los fármacos , Diferenciación Celular/efectos de los fármacos , Hongos Mitospóricos/efectos de los fármacos , Hongos Mitospóricos/fisiología , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/fisiología , Especificidad de la Especie , Esporas Fúngicas , Trichoderma/fisiologíaRESUMEN
Thymosin fraction 5 from the Research Institute for Pharmacy and Biochemistry possessed the immunomodulatory activity in performed tests. The activity is manifested mostly by the induction of the maturation and the differentiation of T cell precursors, which are released from thymus to secondary lymphoid organs. Thymosin fraction 5 causes the expression of the surface membrane markers Thy-1.2, Lyt-2 and L3T4 on the immature T lymphocytes as well. The induced cells are immunocompetent and able to exert the complete functional activity. In the intact mice thymosin fraction 5 had not pronounced effect on the functional potency of mature immunocompetent cells, but in MTA influenced mice its stimulatory effect on the proliferative response to mitogens and on the antibody production against the T-dependent antigen was demonstrated. Thymosin fraction 5 influenced positively not only the components of the specific immunity, but also the functional activity of peritoneal macrophages. The toxicity of thymosin fraction 5 was in the battery of tests in vitro and in vivo very low and therefore it can be considered to be harmless for the clinical practice.
Asunto(s)
Adyuvantes Inmunológicos , Timosina/análogos & derivados , Animales , Formación de Anticuerpos/efectos de los fármacos , Reacción Injerto-Huésped/efectos de los fármacos , Inmunidad/efectos de los fármacos , Activación de Linfocitos/efectos de los fármacos , Linfocitos/efectos de los fármacos , Linfocitos/inmunología , Ratones , Ratones Endogámicos , Timosina/farmacología , Timosina/toxicidadAsunto(s)
Nucléolo Celular/efectos de los fármacos , Indometacina/farmacología , Linfocitos/efectos de los fármacos , Mercaptopurina/farmacología , Fenilbutazona/farmacología , Pirazoles/farmacología , Adyuvantes Inmunológicos , Animales , Artropatías , Cetonas/farmacología , Masculino , ARN/análisis , RatasAsunto(s)
Antiinflamatorios/metabolismo , Fenilacetatos/metabolismo , Animales , Biotransformación , Perros , Humanos , Cinética , Conejos , Ratas , Ratas Endogámicas , Especificidad de la EspecieAsunto(s)
Antiinflamatorios/farmacología , Plaquetas/efectos de los fármacos , Dicumarol/farmacología , Fibrinólisis/efectos de los fármacos , Adhesividad Plaquetaria/efectos de los fármacos , Antiinflamatorios/uso terapéutico , Aspirina/farmacología , Membrana Celular/efectos de los fármacos , Cloroquina/farmacología , Cumarinas/farmacología , Depresión Química , Eritrocitos/efectos de los fármacos , Ácido Flufenámico/farmacología , Hemólisis/efectos de los fármacos , Humanos , Soluciones Hipotónicas , Técnicas In Vitro , Indometacina/farmacología , Inflamación/tratamiento farmacológico , Ácido Mefenámico/farmacología , Oxifenilbutazona/farmacología , Fenilbutazona/farmacología , Fenilpropionatos/farmacología , Pirazoles/farmacología , Receptores de Droga , Relación Estructura-ActividadAsunto(s)
Antiinflamatorios/farmacología , Coagulación Sanguínea/efectos de los fármacos , Cumarinas/farmacología , Tiempo de Protrombina , Pirazoles/farmacología , Adenosina Difosfato/farmacología , Administración Oral , Animales , Pruebas de Coagulación Sanguínea , Cumarinas/sangre , Relación Dosis-Respuesta a Droga , Interacciones Farmacológicas , Sinergismo Farmacológico , Biscumacetato de Etilo/farmacología , Técnicas In Vitro , Inyecciones Subcutáneas , Fenilbutazona/administración & dosificación , Fenilbutazona/sangre , Fenilbutazona/farmacología , Adhesividad Plaquetaria/efectos de los fármacos , Conejos , Ratas , Warfarina/administración & dosificación , Warfarina/metabolismo , Warfarina/farmacologíaRESUMEN
Antiinflammatory activities of low- and high- molecular-weight polymers of o-methacryloyloxybenzoic acid were compared with the antiinflammatory activity of o-acetoxybenzoic (acetylsalicylic) acid in tests on hind-leg inflammations in rats induced by injection of either Freund's adjuvant or aqueous suspension of kaolin. The antiinflammatory activies of the orally administered polymers cooresponded to 40-90% of the activity of o-acetoxybenzoic acid, and were not substantially dependent on the degree of polymerization.
Asunto(s)
Resinas Acrílicas/uso terapéutico , Inflamación/tratamiento farmacológico , Ácidos Polimetacrílicos/uso terapéutico , Animales , Aspirina/uso terapéutico , RatasRESUMEN
A new potential antiinflammatory substance serves as an example that substantiates the necessity of carrying out concurrent comparative pharmacokinetic and biotransformation studies of new drugs in experimental animals and man during the preclinical research stage, with adherence to all safety precautions. Such a procedure will facilitate the choice of an animal model that approaches human metabolism and pharmacokinetics as closely as possible. This serves in the further development of the test drug, and thus enhances the validity of the transfer of experimental data to man, especially from the toxicological aspect.
Asunto(s)
Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos , Adulto , Animales , Biotransformación , Embrión de Pollo , Evaluación Preclínica de Medicamentos , Femenino , Humanos , Cinética , Masculino , Preparaciones Farmacéuticas/metabolismo , Fenilacetatos/efectos adversos , Fenilacetatos/metabolismo , Fenilacetatos/toxicidad , Conejos , Ratas , Ratas Endogámicas , Especificidad de la EspecieRESUMEN
Consupren Spofa was demonstrated in our tests to be a distinguished immunomudulating agent. The preparation did not show any direct lymphotoxicity or myelotoxicity but it markedly influenced maturation of T lymphocytes in the thymus. When testing the functional activity of the immunity system in vivo, the inhibitory effect of Consupren Spofa was manifested only when administering a relatively larger dose of the drug with an antigenic stimulus or shortly after it, i.e., in the period of early activating processes. The use of a suitable time and dosage regimen is therefore decisive to induce an inhibitory effect. On the basis of comparative experiments it can be stated that Consupren Spofa is minimally as much effective as, or in some experiments even more effective than Sandimmun Sandoz.
Asunto(s)
Ciclosporina/farmacología , Inmunidad/efectos de los fármacos , Animales , Formación de Anticuerpos/efectos de los fármacos , Femenino , Reacción Injerto-Huésped/efectos de los fármacos , Recuento de Leucocitos , Activación de Linfocitos/efectos de los fármacos , Tejido Linfoide/efectos de los fármacos , Ratones , Ratones Endogámicos C57BLRESUMEN
The new antirheumatic drugs Benzofenac and Flobufen were assessed for their possible influence on various immunological parameters. They were shown to enhance passive cutaneous anaphylaxis (PCA) to ovalbumin in rats and to suppress the numbers of helper and suppressor T-lymphocytes in mice. The effect of Benzofenac in the latter system was found to be more pronounced. The drugs had no effects in any of the other test systems, including the development of delayed hypersensitivity, mitogen-induced lymphocyte proliferation in vitro and the production of antisera (as tested by PCA).