RESUMEN
Mycobacterium tuberculosis is a bacterial pathogen that can persist within an infected individual for extended periods of time without causing overt, clinical disease, in a state normally referred to as latent or chronic tuberculosis. Although the replicative state of the bacterium during this period is a matter of some conjecture, recent developments have indicated that the bacterium requires the regulated expression of a set of genes and metabolic pathways to maintain a persistent infection in an immunocompetent host. The characterization of these gene products and their role in bacterial metabolism and physiology is starting to provide insights into the mechanisms that M. tuberculosis has evolved to adopt its highly successful mode of pathogenicity.
Asunto(s)
Adaptación Fisiológica , Mycobacterium tuberculosis/fisiología , Mycobacterium tuberculosis/patogenicidad , Tuberculosis/microbiología , Animales , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Enfermedad Crónica , Regulación Bacteriana de la Expresión Génica , Humanos , Mycobacterium tuberculosis/genética , Factores de TiempoRESUMEN
Human trophoblast research relies on a combination of in vitro models, including isolated primary cultures, explant cultures, and trophoblast cell lines. In the present study, we have utilized the rotating wall vessel (RWV) bioreactor to generate a three-dimensional (3-D) model of human placentation for the study of cytotrophoblast (CTB) invasion. The RWV supported the growth of the human CTB cell line SGHPL-4 and allowed for the formation of complex, multilayered 3-D aggregates that were morphologically, phenotypically, and functionally distinct from SGHPL-4 monolayers. The cells cultured three-dimensionally differentiated into an aggressively invasive cell population characterized by the upregulation of matrix metalloproteinase-2 (MMP-2), MMP-3, MMP-9 and urokinase-type plasminogen activator (uPA) secretion and activation. Microarray analysis of the 3-D and 2-D cultured cells revealed increased expression in the 3-D cells of various genes that are known mediators of invasion, including MT1-MMP, PECAM-1 and L-selectin, as well as genes not previously associated with CTB differentiation such as MMP-13 and MT5-MMP. These results were verified by quantitative real-time PCR. These findings suggest that when cultured in 3-D, SGHPL-4 cells closely mimic differentiating in utero CTBs, providing a novel approach for the in vitro study of the molecular mechanisms that regulate CTB differentiation and invasion.
Asunto(s)
Placentación/fisiología , Trofoblastos/citología , Reactores Biológicos , Western Blotting , Agregación Celular/fisiología , Diferenciación Celular/fisiología , Procesos de Crecimiento Celular/fisiología , Línea Celular , Femenino , Humanos , Selectina L/biosíntesis , Selectina L/genética , Metaloproteinasas de la Matriz/genética , Metaloproteinasas de la Matriz/metabolismo , Microscopía Electrónica de Rastreo , Microscopía Fluorescente , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/biosíntesis , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/genética , Embarazo , ARN Mensajero/biosíntesis , ARN Mensajero/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Trofoblastos/enzimología , Trofoblastos/metabolismo , Trofoblastos/ultraestructura , Activador de Plasminógeno de Tipo Uroquinasa/genética , Activador de Plasminógeno de Tipo Uroquinasa/metabolismoRESUMEN
A comprehensive analysis of both the molecular genetic and phenotypic responses of any organism to the space flight environment has never been accomplished because of significant technological and logistical hurdles. Moreover, the effects of space flight on microbial pathogenicity and associated infectious disease risks have not been studied. The bacterial pathogen Salmonella typhimurium was grown aboard Space Shuttle mission STS-115 and compared with identical ground control cultures. Global microarray and proteomic analyses revealed that 167 transcripts and 73 proteins changed expression with the conserved RNA-binding protein Hfq identified as a likely global regulator involved in the response to this environment. Hfq involvement was confirmed with a ground-based microgravity culture model. Space flight samples exhibited enhanced virulence in a murine infection model and extracellular matrix accumulation consistent with a biofilm. Strategies to target Hfq and related regulators could potentially decrease infectious disease risks during space flight missions and provide novel therapeutic options on Earth.
Asunto(s)
Salmonella typhimurium/genética , Salmonella typhimurium/patogenicidad , Vuelo Espacial , Animales , Biopelículas/crecimiento & desarrollo , Femenino , Expresión Génica , Genes Bacterianos , Proteína de Factor 1 del Huésped/fisiología , Hierro/metabolismo , Ratones , Ratones Endogámicos BALB C , Análisis de Secuencia por Matrices de Oligonucleótidos , Proteómica , Regulón , Salmonelosis Animal/etiología , Salmonella typhimurium/fisiología , Virulencia , Simulación de IngravidezRESUMEN
A three-dimensional (3-D) lung aggregate model was developed from A549 human lung epithelial cells by using a rotating-wall vessel bioreactor to study the interactions between Pseudomonas aeruginosa and lung epithelial cells. The suitability of the 3-D aggregates as an infection model was examined by immunohistochemistry, adherence and invasion assays, scanning electron microscopy, and cytokine and mucoglycoprotein production. Immunohistochemical characterization of the 3-D A549 aggregates showed increased expression of epithelial cell-specific markers and decreased expression of cancer-specific markers compared to their monolayer counterparts. Immunohistochemistry of junctional markers on A549 3-D cells revealed that these cells formed tight junctions and polarity, in contrast to the cells grown as monolayers. Additionally, the 3-D aggregates stained positively for the production of mucoglycoprotein while the monolayers showed no indication of staining. Moreover, mucin-specific antibodies to MUC1 and MUC5A bound with greater affinity to 3-D aggregates than to the monolayers. P. aeruginosa attached to and penetrated A549 monolayers significantly more than the same cells grown as 3-D aggregates. Scanning electron microscopy of A549 cells grown as monolayers and 3-D aggregates infected with P. aeruginosa showed that monolayers detached from the surface of the culture plate postinfection, in contrast to the 3-D aggregates, which remained attached to the microcarrier beads. In response to infection, proinflammatory cytokine levels were elevated for the 3-D A549 aggregates compared to monolayer controls. These findings suggest that A549 lung cells grown as 3-D aggregates may represent a more physiologically relevant model to examine the interactions between P. aeruginosa and the lung epithelium during infection.
Asunto(s)
Células Epiteliales/microbiología , Pulmón/microbiología , Modelos Biológicos , Infecciones por Pseudomonas , Antígenos/metabolismo , Antígenos de Neoplasias , Biomarcadores , Reactores Biológicos , Técnicas de Cultivo de Célula/métodos , Colágeno Tipo IV/metabolismo , Células Epiteliales/metabolismo , Glicoproteínas/metabolismo , Humanos , Interleucinas/metabolismo , Laminina/metabolismo , Pulmón/metabolismo , Mucina 5AC , Mucina-1 , Mucinas/metabolismo , Pseudomonas aeruginosa , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
The malK gene, encoding a membrane-associated component of the maltose transport complex of Salmonella typhimurium was cloned into an expression vector downstream of the promoters lambda pR and lambda pL and a strong translation initiation region. Escherichia coli strain JM109 harboring the resulting plasmid pCW14 synthesized a protein of apparent molecular mass of 43 kDa upon temperature shift, as demonstrated by sodium dodecyl sulfate-gel electrophoresis. The identity of the protein was determined by N-terminal amino acid sequencing. The overproduced protein was sequestered in inclusion bodies as revealed by electron microscopy. The protein was purified to homogeneity on a large scale by disrupting the cells with a passage through a Ribi press, solubilizing the inclusion bodies with urea, and subsequent chromatography on Red Agarose. Purified MalK, as the membrane-bound MalK protein could be covalently modified by [gamma-32P]8-azido-ATP. Furthermore, the purified protein bound [gamma-32P] ATP with a dissociation constant of 150 microM and exhibited ATPase activity, which was stimulated by dimethyl-sulfoxide and inhibited by ADP.
Asunto(s)
Transportadoras de Casetes de Unión a ATP , Adenosina Trifosfatasas/metabolismo , Proteínas Bacterianas/metabolismo , Proteínas Portadoras/metabolismo , Proteínas de Escherichia coli , Maltosa/metabolismo , Nucleótidos/metabolismo , Salmonella typhimurium/metabolismo , Adenosina Trifosfato/metabolismo , Proteínas Bacterianas/aislamiento & purificación , Secuencia de Bases , Proteínas Portadoras/aislamiento & purificación , Cromatografía Liquida , Clonación Molecular , Electroforesis en Gel de Poliacrilamida , Escherichia coli/genética , Microscopía Electrónica , Datos de Secuencia Molecular , Plásmidos , Regiones Promotoras Genéticas , Biosíntesis de Proteínas , Especificidad por SustratoRESUMEN
A clinical isolate of Aeromonas hydrophila was demonstrated to transport [14C]maltose with similar kinetics to enteric bacteria (Km: 0.3 microM; Vmax: 22 nmol min-1 per 10(9) cells). The uptake of [14C]maltose was completely inhibited in the presence of unlabelled maltose or maltodextrins, whereas other mono- and disaccharides, such as glucose, galactose, sucrose, lactose or melibiose, had no effect. A protein with an apparent molecular mass of 39 kDa (maltose-binding protein; MBP) was identified in osmotic-shock fluid of maltose-grown cells by SDS-gel electrophoresis, and was purified to homogeneity by either amylose affinity chromatography or ion-exchange chromatography. Equilibrium dialysis experiments revealed the ability of the purified protein to bind [14C]maltose with high affinity (KD = 1.6 microM). Unlabelled maltose and maltodextrins competed for the binding site. In a reconstitution experiment, A. hydrophila MBP poorly restored the transport activity of a binding-protein-deficient Escherichia coli (delta malE) mutant. N-terminal sequence analyses of the purified native protein and of peptides generated by cleavage with CNBr and subsequently separated by HPLC revealed about 56% identical amino acid residues, as compared to enterobacterial MBPs. We conclude that maltose is transported into A. hydrophila via a binding-protein-dependent transport system.
Asunto(s)
Transportadoras de Casetes de Unión a ATP , Aeromonas hydrophila/metabolismo , Proteínas Bacterianas/metabolismo , Proteínas Portadoras/metabolismo , Proteínas de Escherichia coli , Maltosa/metabolismo , Proteínas de Transporte de Monosacáridos , Proteínas de Unión Periplasmáticas , Secuencia de Aminoácidos , Transporte Biológico/efectos de los fármacos , Metabolismo de los Hidratos de Carbono , Carbohidratos/farmacología , Proteínas Portadoras/química , Escherichia coli/genética , Escherichia coli/metabolismo , Prueba de Complementación Genética , Maltosa/farmacología , Proteínas de Unión a Maltosa , Datos de Secuencia Molecular , Alineación de Secuencia , Homología de Secuencia de AminoácidoRESUMEN
The ATP-binding protein, MalK, of the bacterial ABC (ATP-binding-cassette) transport complex MalFGK2 provides the energy for the translocation of maltose and maltodextrins across the cytoplasmic membrane. The MalK protein from Salmonella typhimurium was overexpressed in Escherichia coli and crystallized by the hanging-drop method using (NH4)2SO4as a precipitant. The crystals belong to space group P6x22 (most probably x = 1 or 5) with cell dimensions a = 181.8 and c = 182.5 A, corresponding to three or four molecules per asymmetric unit. They diffract to a resolution of about 3 A on a synchrotron X-ray source and are suitable for structure determination.
Asunto(s)
Transportadoras de Casetes de Unión a ATP/química , Transportadoras de Casetes de Unión a ATP/aislamiento & purificación , Proteínas Bacterianas/química , Proteínas Bacterianas/aislamiento & purificación , Proteínas de Escherichia coli , Transportadoras de Casetes de Unión a ATP/genética , Proteínas Bacterianas/genética , Cristalización , Cristalografía por Rayos X , Escherichia coli/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Salmonella typhimurium/genéticaRESUMEN
Energy transduction in the anaerobic, thermophilic bacterium Clostridium fervidus relies exclusively on Na+ as the coupling ion. The Na+ ion gradient across the membrane is generated by a membrane-bound ATPase (G. Speelmans, B. Poolman, T. Abee, and W. N. Konings, J. Bacteriol. 176:5160-5162, 1994). The Na+-ATPase complex was purified to homogeneity. It migrates as a single band in native polyacrylamide gel electrophoresis and catalyzes Na+-stimulated ATPase activity. Denaturing gel electrophoresis showed that the complex consists of at least six different polypeptides with apparent molecular sizes of 66, 61, 51, 37, 26, and 17 kDa. The N-terminal sequences of the 66- and 51-kDa subunits were found to be significantly homologous to subunits A and B, respectively, of the Na+-translocating V-type ATPase of Enterococcus hirae. The purified V1V0 protein complex was reconstituted in a mixture of Escherichia coli phosphatidylethanolamine and egg yolk phosphatidylcholine and shown to catalyze the uptake of Na+ ions upon hydrolysis of ATP. Na+ transport was completely abolished by monensin, whereas valinomycin stimulated the uptake rate. This is indicative of electrogenic sodium transport. The presence of the protonophore SF6847 had no significant effect on the uptake, indicating that Na+ translocation is a primary event and in the cell is not accomplished by an H+-translocating pump in combination with an Na+-H+ antiporter.
Asunto(s)
Adenosina Trifosfatasas/metabolismo , Proteínas de Transporte de Catión , Clostridium/enzimología , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Sodio/metabolismo , Adenosina Trifosfatasas/química , Adenosina Trifosfatasas/aislamiento & purificación , Secuencia de Aminoácidos , Transporte Biológico Activo/efectos de los fármacos , Electroforesis en Gel de Poliacrilamida , Ionóforos/farmacología , Datos de Secuencia Molecular , Peso Molecular , Monensina/farmacología , Nitrilos/farmacología , Proteolípidos , ATPasa Intercambiadora de Sodio-Potasio/química , ATPasa Intercambiadora de Sodio-Potasio/aislamiento & purificación , Valinomicina/farmacologíaRESUMEN
Analysis by two-dimensional gel electrophoresis revealed that Mycobacterium avium expresses several proteins unique to an intracellular infection. One abundant protein with an apparent molecular mass of 50 kDa was isolated, and the N-terminal sequence was determined. It matches a sequence in the M. tuberculosis database (Sanger) with similarity to the enzyme isocitrate lyase of both Corynebacterium glutamicum and Rhodococcus fascians. Only marginal similarity was observed between this open reading frame (ORF) (termed icl) and a second distinct ORF (named aceA) which exhibits a low similarity to other isocitrate lyases. Both ORFs can be found as distinct genes in the various mycobacterial databases recently published. Isocitrate lyase is a key enzyme in the glyoxylate cycle and is essential as an anapleurotic enzyme for growth on acetate and certain fatty acids as carbon source. In this study we express and purify Icl, as well as AceA proteins, and show that both exhibit isocitrate lyase activity. Various known inhibitors for isocitrate lyase were effective. Furthermore, we present evidence that in both M. avium and M. tuberculosis the production and activity of the isocitrate lyase is enhanced under minimal growth conditions when supplemented with acetate or palmitate.
Asunto(s)
Proteínas Bacterianas , Isocitratoliasa/metabolismo , Mycobacterium avium/enzimología , Mycobacterium tuberculosis/enzimología , Acetatos/metabolismo , Clonación Molecular , Escherichia coli/metabolismo , Regulación Enzimológica de la Expresión Génica , Concentración de Iones de Hidrógeno , Isocitratoliasa/química , Isocitratoliasa/aislamiento & purificación , Isoenzimas/química , Isoenzimas/aislamiento & purificación , Isoenzimas/metabolismo , Cinética , Sistemas de Lectura Abierta , Palmitatos/metabolismo , Proteínas Recombinantes/metabolismo , Ácido Succínico/metabolismoRESUMEN
Mycobacterium tuberculosis claims more human lives each year than any other bacterial pathogen. Infection is maintained in spite of acquired immunity and resists eradication by antimicrobials. Despite an urgent need for new therapies targeting persistent bacteria, our knowledge of bacterial metabolism throughout the course of infection remains rudimentary. Here we report that persistence of M. tuberculosis in mice is facilitated by isocitrate lyase (ICL), an enzyme essential for the metabolism of fatty acids. Disruption of the icl gene attenuated bacterial persistence and virulence in immune-competent mice without affecting bacterial growth during the acute phase of infection. A link between the requirement for ICL and the immune status of the host was established by the restored virulence of delta icl bacteria in interferon-gamma knockout mice. This link was apparent at the level of the infected macrophage: Activation of infected macrophages increased expression of ICL, and the delta icl mutant was markedly attenuated for survival in activated but not resting macrophages. These data suggest that the metabolism of M. tuberculosis in vivo is profoundly influenced by the host response to infection, an observation with important implications for the treatment of chronic tuberculosis.