Sheep and goat production objectives in pastoral and agro-pastoral production systems in Chifra district of Afar, Ethiopia.

The study was conducted in Chifra district in Awsi-Resu zone of Afar Regional State to explore production objectives and strategies of sheep and goats in pastoral and agro-pastoral production systems. Cross-sectional survey with systematic random selection of 180 households (90 households from each production system was conducted. Descriptive statistics, t test, chi-square test, analysis of variance, and ranking method were employed in analyzing data using JMP-5 software. In pastoral production system, sheep and goat were primarily reared for household milk consumption, followed by live animal savings and income, while in agro-pastoral production system, sheep and goat are primarily reared for income, followed by household milk consumption and manure supply, as live animal savings. Overall mortality rates of sheep and goats (4 to >12 months of age) were 9 and 9.5 % in pastoral production systems while 7 and 6 % in agro-pastoral production system. Average age at first mating, kidding interval, days of mating after birth, weaning age, lactation length, litter size, slaughtering age, and marketable age of sheep and goats in pastoral production system were 7.4 months, 358 days, 29 days, 4.2 months, 3.1 months, 1.5 lambs, 7.4 months, and 14 months, respectively, while in agro-pastoral production system, they are 6.9 months, 353.3 days, 28 days, 3.2 months, 1.6 lambs, 6.4 months, and 14.5 months, respectively. It was concluded that production system-based development interventions could help to increase the productivity of sheep and goats and thereby enhance the livelihood of smallholders.

Genomic characterization of Listeria monocytogenes and Listeria innocua isolated from milk and dairy samples in Ethiopia.

Listeriosis caused by Listeria monocytogenes often poses a significant threat to vulnerable populations. Dairy products have been implicated in outbreaks of listeriosis worldwide. In Ethiopia, studies have identified Listeria spp. and L. monocytogenes in various dairy products, but the genetic diversity and phylogenetic relationships of these bacteria remain largely unknown in the low- and middle-income countries. Therefore, we conducted whole-genome sequencing on 15 L. monocytogenes and 55 L. innocua isolates obtained from different levels of the dairy supply chains across three regions in Ethiopia. Genomes were assembled and used for MLST genotyping and single nucleotide polymorphism (SNP) analysis to infer phylogenetic relationships. We identified a total of 3 L. monocytogenes (i.e., 2, 145, and 18) and 12 L. innocua (i.e., 1489, 1619, 603, 537, 1010, 3186, 492, 3007, 1087, 474, 1008, and 637) MLST sequence types among the studied isolates. Some of these sequence types showed region-specific occurrence, while others were broadly distributed across regions. Through high-quality SNP analysis, we found that among 13 L. monocytogenes identified as ST 2, 11 of them were highly similar with low genetic variation, differing by only 1 to 10 SNPs, suggesting potential selection in the dairy food supply chain. The L. innocua isolates also exhibited low intra-ST genetic variation with only 0-10 SNP differences, except for the ST 1619, which displayed a greater diversity.