RESUMEN
OBJECTIVE: To study the effect of Rhizoma Drynaria ethanol extract on regulation of cellular immune functions in cyclophosphamide-induced immunosuppressive mice. METHODS: A total of 60 kunming mice were randomly divided into six groups:normal control group, model group, positive control group( levamisole, 0. 05 g / kg) and low-, medium- and high-dose Rhizoma Drynaria ethanol extract-treated groups( 2. 5, 5, 10 g / kg). The mice in the four administration group were administered with 10 mL / kg of the drug for 20 days. Except normal control group, the mice in the other 5 groups were intraperitoneally injected( ip) with cyclophosphamide to establish immunosuppression model. After the end of the experiment, the indices of lymphocyte proliferation capacity and delayed type hypersensitivity were detected, speen histological structure and Tlymphocyte subsets CD_4~+, CD_8~+T cells in peripheral blood were measured. The relativemRNA expression of Interleukin 4( IL-4) and Interferon-γ( IFN-γ) in the spleen tissues were detected. RESULTS: Rhizoma Drynaria ethanol extract( 5, 10 g / kg) enhanced Con Ainduced T-lymphocytes proliferative capacity and delayed type hypersensitivity, increased CD_4~+T cells, CD_4~+/ CD_8~+ratio in peripheral blood, IFN-γ mRNA expression and IFN-γ /IL-4 ratio, decreased CD_8~+T cells and IL-4 mRNA expression. However, there was no obvious difference in LPS-induced B-lymphocyte proliferation capacity. CONCLUSION: Rhizoma Drynaria ethanol extract could regulate the cellular immune functions in cyclophosphamide-induced immunosuppressive mice.