Asunto(s)
Antígenos HLA/genética , Prueba de Histocompatibilidad/métodos , Secuencia de Bases , Dermatoglifia del ADN , Antígenos HLA/inmunología , Antígenos HLA-DQ/genética , Cadenas alfa de HLA-DQ , Cadenas beta de HLA-DQ , Humanos , Prueba de Cultivo Mixto de Linfocitos , Datos de Secuencia Molecular , Oligodesoxirribonucleótidos , Reacción en Cadena de la Polimerasa/métodos , Polimorfismo de Longitud del Fragmento de RestricciónRESUMEN
Three new human leukocyte antigen (HLA) class I alleles have been identified in the Tissue Typing Laboratory in Sydney, Australia. Sequence analysis of exon 2 and exon 3 of the HLA-B gene revealed the novel polymorphism. A silent substitution of C to T at nucleotide position 369 has been identified for the HLA-B*400104 allele when compared to the closest matched allele, HLA-B*400101. The HLA-B*3928 allele was identified with a nucleotide substitution of G to C at position 362 when compared to the closest matched allele, HLA-B*390101, resulting in an amino acid substitution of Arginine to Threonine. A nucleotide substitution of C to G at position 572 resulting in the amino acid change Serine to Tryptophan was identified in the new allele HLA-B*4437, when compared to the closest matched allele HLA-B*440301. Both amino acid substitutions implicate a different specificity and affinity of antigen binding for the alleles HLA-B*3928 and HLA-B*4437.
Asunto(s)
Alelos , Sondas de ADN de HLA/genética , Variación Genética , Antígenos HLA-B/genética , Sustitución de Aminoácidos/genética , Secuencia de Bases , Exones/genética , Humanos , Datos de Secuencia Molecular , Análisis de Secuencia de ADNRESUMEN
Anew human leukocyte antigen-B allele, B*1565, has been identified during routine typing of cord blood samples. Subsequently, two individuals from the same family as the first cord blood sample plus two unrelated Australian Bone Marrow Donor Registry samples have been found to carry this novel allele.
Asunto(s)
Antígenos HLA-B/genética , Secuencia de Aminoácidos , Secuencia de Bases , Exones , Antígeno HLA-B15 , Humanos , Datos de Secuencia MolecularRESUMEN
Anew human leukocyte antigen (HLA) class I allele, HLA-A*0252, has been found during routine typing of samples for the Australian Bone Marrow Donor Registry. A*0252 differs from A*020101 at four codon positions, with all the new polymorphisms resulting in an amino acid change. The amino acids involved are located in the antigen-binding region of the HLA protein.
Asunto(s)
Antígenos HLA-A/genética , Secuencia de Bases , Exones , Antígeno HLA-A2 , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la PolimerasaRESUMEN
Three novel alleles, human leukocyte antigen (HLA)-B*0725, B*0728, and B*3808, were discovered during routine genotyping of samples for the Australian Bone Marrow Donor Registry and Australian Cord Blood Bank. The new alleles contain amino acid changes in the antigen-binding site of the expressed HLA protein, which may alter the antigen-binding properties of the functional protein.
Asunto(s)
Antígenos HLA-B/genética , Secuencia de Bases , Antígeno HLA-B38 , Antígeno HLA-B7 , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la PolimerasaRESUMEN
In this article, we report two new human leukocyte antigen-C (HLA-C) alleles, HLA-Cw*0314 and Cw*1511, which were identified during routine tissue typing of donors for the Australian Bone Marrow Donor Registry and Australian Cord Blood Bank. HLA-Cw*0314 shows six codon changes in exon 3 compared to Cw*030401 and shares some sequence homology with Cw*07 alleles. Cw*1511 has two nucleotide changes compared with Cw*150201 in exon 2, both resulting in amino acid changes in the protein sequence.