RESUMEN
Differential patterns of cellular development and function are determined, at least in part, by the specific gene expression of particular cells. Thus, determination of differential patterns of gene expression between tissues is likely to help elucidate molecular details of tissue-specific processes. Our hypothesis was that cells of the circumvallate papilla involved in taste perception would express genes that are not expressed in the surrounding epithelium and that determination of the nature of these genes could be helpful in our understanding of the molecular details of taste. Using partial sequencing of clones derived from rat circumvallate papillae, we have begun to characterize genes that could be important in taste. We prepared a cDNA library of whole circumvallate papillae and, by means of a novel subtraction procedure, enriched taste-specific clones. Characterization of the libraries showed that subtraction resulted in good enrichment of taste-specific clones. Here we report the partial sequencing and analysis of 410 cDNA clones from the taste-bud-enriched cDNA library. Approximately 25% of the genes were identified on the basis of their high homology to known transcripts. These included the developmentally important molecules Pax-1, esp1, Notch 1, and Notch 3 that may play roles in the continuous turnover of taste receptor cells. A further 20% of the genes had no significant homology to known DNA sequences and were identified as taste-specific by Southern blot analysis.
Asunto(s)
Papilas Gustativas/química , Gusto/genética , Animales , Secuencia de Bases , Células Quimiorreceptoras/química , Células Clonales , Expresión Génica , Biblioteca de Genes , Encía/citología , Masculino , Datos de Secuencia Molecular , Especificidad de Órganos , Técnica del ADN Polimorfo Amplificado Aleatorio , Ratas , Ratas Wistar , Análisis de Secuencia de ADN/métodos , Homología de Secuencia de Ácido Nucleico , Papilas Gustativas/citologíaRESUMEN
The genome of Rhodopseudomonas palustris contains five antenna gene clusters, alpha beta a, alpha beta b, alpha beta c, alpha beta d and alpha beta e, which encode the light-harvesting peripheral antenna complex II polypeptides. The isolation and characterisation of the gene which encodes the alpha e and beta e polypeptides are reported. The primary structure of the beta e polypeptide is identical to that of beta b whilst the structure of alpha e is different from the other alpha subunits so far characterised. All five of the gene clusters were transcribed under high-light conditions while under low-light conditions only three were transcribed (alpha beta b, alpha beta d and alpha beta e). Furthermore, Northern-blot analysis showed that the gene clusters encode RNA transcripts of either 500 or 650 nucleotides. Individual members of the gene family showed a differential response in terms of the regulation of abundance of mRNA upon growth under either high-light or low-light intensities. Possible promoter sequences and operator sites upstream of the alpha beta b, alpha beta d and alpha beta e genes were located. Furthermore using puc-lacZ fusions in trans in R. palustris, we were able to examine the positions of the promoter of the gene clusters. The significance of these observations with respect to the regulation, organization and role of the peripheral antenna is discussed.
Asunto(s)
Familia de Multigenes , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Rhodopseudomonas/genética , Secuencia de Aminoácidos , Secuencia de Bases , Northern Blotting , Clonación Molecular , ADN Bacteriano , Genes Bacterianos , Datos de Secuencia Molecular , Regiones Promotoras Genéticas , ARN/metabolismo , Análisis de Secuencia , Homología de Secuencia de Aminoácido , Análisis EspectralRESUMEN
The taste-specific G-protein alpha-subunit, alpha-gustducin, was expressed using a baculovirus based system. alpha-Gustducin was demonstrated to be myristoylated and was also palmitoylated in insect larval cells. Recombinant alpha-gustducin was purified to homogeneity. Neither receptors nor effectors that interact with gustducin in taste are known. However, alpha-gustducin has a close structural similarity to the visual G-protein, alpha-transducin. Therefore alpha-gustducin was reconstituted with components of the visual system to determine the degree of its functional similarity with alpha-transducin. Despite the fact that the sequences of alpha-gustducin and alpha-transducin share only 80% identity with each other, the interactions and functions of these two proteins were quantitatively identical. These included the interaction with receptor, bovine rhodopsin, with effector, bovine retinal cyclic GMP-phosphodiesterase, and with bovine brain and retinal G-protein beta gamma-heterodimers; receptor-catalysed GDP-GTP exchange and the intrinsic GTPase activity of alpha-gustducin and alpha-transducin were also identical. Gi alpha which is 70% identical with alpha-transducin interacts with different receptor and effector proteins and has very different guanine-nucleotide binding properties. Therefore, the functional equivalence of alpha-gustducin and alpha-transducin suggest that taste buds are likely to contain receptor and effector proteins that share many properties with their retinal equivalents.
Asunto(s)
Gusto , Transducina/genética , 3',5'-AMP Cíclico Fosfodiesterasas/metabolismo , Adenosina Difosfato Ribosa/metabolismo , Animales , Baculoviridae/genética , Secuencia de Bases , Catálisis , Línea Celular , Clonación Molecular , Cartilla de ADN , ADN Complementario , Activación Enzimática , GTP Fosfohidrolasas/metabolismo , Guanosina 5'-O-(3-Tiotrifosfato)/metabolismo , Datos de Secuencia Molecular , Unión Proteica , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Rodopsina/metabolismo , Spodoptera , Transducina/aislamiento & purificación , Transducina/metabolismo , Factores de Virulencia de Bordetella/farmacologíaRESUMEN
The sense of taste provides animals with valuable information about the quality and nutritional value of food. Previously, we identified a large family of mammalian taste receptors involved in bitter taste perception (the T2Rs). We now report the characterization of mammalian sweet taste receptors. First, transgenic rescue experiments prove that the Sac locus encodes T1R3, a member of the T1R family of candidate taste receptors. Second, using a heterologous expression system, we demonstrate that T1R2 and T1R3 combine to function as a sweet receptor, recognizing sweet-tasting molecules as diverse as sucrose, saccharin, dulcin, and acesulfame-K. Finally, we present a detailed analysis of the patterns of expression of T1Rs and T2Rs, thus providing a view of the representation of sweet and bitter taste at the periphery.
Asunto(s)
Receptores de Superficie Celular/metabolismo , Receptores Acoplados a Proteínas G , Edulcorantes , Papilas Gustativas/química , Papilas Gustativas/metabolismo , Gusto/fisiología , Animales , Señalización del Calcio , Línea Celular , Cromosomas Humanos Par 4/genética , Cricetinae , Evolución Molecular , Perfilación de la Expresión Génica , Prueba de Complementación Genética , Proteínas de Unión al GTP Heterotriméricas/genética , Proteínas de Unión al GTP Heterotriméricas/metabolismo , Humanos , Ratones , Ratones Transgénicos , Datos de Secuencia Molecular , Familia de Multigenes , Compuestos de Fenilurea/metabolismo , Mapeo Físico de Cromosoma , ARN Mensajero/genética , ARN Mensajero/metabolismo , Receptores de Superficie Celular/química , Receptores de Superficie Celular/genética , Sacarina/metabolismo , Especificidad por Sustrato , Sacarosa/metabolismo , Papilas Gustativas/citología , Tiazinas/metabolismoRESUMEN
In mammals, taste perception is a major mode of sensory input. We have identified a novel family of 40-80 human and rodent G protein-coupled receptors expressed in subsets of taste receptor cells of the tongue and palate epithelia. These candidate taste receptors (T2Rs) are organized in the genome in clusters and are genetically linked to loci that influence bitter perception in mice and humans. Notably, a single taste receptor cell expresses a large repertoire of T2Rs, suggesting that each cell may be capable of recognizing multiple tastants. T2Rs are exclusively expressed in taste receptor cells that contain the G protein alpha subunit gustducin, implying that they function as gustducin-linked receptors. In the accompanying paper, we demonstrate that T2Rs couple to gustducin in vitro, and respond to bitter tastants in a functional expression assay.
Asunto(s)
Células Quimiorreceptoras/fisiología , Proteínas de la Membrana/genética , Receptores de Superficie Celular/genética , Receptores Acoplados a Proteínas G , Papilas Gustativas/fisiología , Gusto/fisiología , Animales , Células Quimiorreceptoras/química , Clonación Molecular , Evolución Molecular , Proteínas de Unión al GTP/metabolismo , Expresión Génica/fisiología , Humanos , Mamíferos , Proteínas de la Membrana/análisis , Proteínas de la Membrana/metabolismo , Ratones , Datos de Secuencia Molecular , Familia de Multigenes/fisiología , ARN Mensajero/análisis , Ratas , Receptores de Superficie Celular/análisis , Receptores de Superficie Celular/metabolismo , Homología de Secuencia de Aminoácido , Transducina/análisis , Transducina/genética , Transducina/metabolismoRESUMEN
Taste represents a major form of sensory input in the animal kingdom. In mammals, taste perception begins with the recognition of tastant molecules by unknown membrane receptors localized on the apical surface of receptor cells of the tongue and palate epithelium. We report the cloning and characterization of two novel seven-transmembrane domain proteins expressed in topographically distinct subpopulations of taste receptor cells and taste buds. These proteins are specifically localized to the taste pore and are members of a new group of G protein-coupled receptors distantly related to putative mammalian pheromone receptors. We propose that these genes encode taste receptors.
Asunto(s)
Proteínas de Unión al GTP/genética , Receptores de Superficie Celular/genética , Receptores Acoplados a Proteínas G , Células Receptoras Sensoriales/química , Células Receptoras Sensoriales/fisiología , Papilas Gustativas/química , Papilas Gustativas/fisiología , Animales , Anticuerpos , Expresión Génica/fisiología , Humanos , Hibridación in Situ , Mamíferos , Ratones , Datos de Secuencia Molecular , Neuronas Aferentes/química , Neuronas Aferentes/fisiología , ARN Mensajero/análisis , Conejos , Ratas , Receptores de Superficie Celular/análisis , Receptores de Superficie Celular/inmunología , Homología de Secuencia de Aminoácido , Transducción de Señal/fisiología , Transducina/análisisRESUMEN
Treatment of outer segment membranes from Loligo forbesi with endoprotease-V8 from Staphylococcus aureus results in cleavage of the C-terminal extension of the squid rhodopsin, with accompanying reduction of the apparent molecular weight from 47,000 to 36,000 on sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Negative-stain electron microscopy of the intact membranes shows that small clusters of the rhodopsin C-termini form structures extending from the membrane surface and that these are absent after protease treatment. Fourier transform infrared spectra of the amide I band of the protein indicate that removal of the C-terminal extension increases the relative alpha-helical content of squid rhodopsin to a level comparable to that for bovine rhodopsin in disk membranes, and to an extent which suggests that the alpha-helical structure lies mainly in the M(r) 36,000 (transmembrane) section of the protein. Saturation-transfer electron spin resonance (ESR) spectroscopy of the spin-labeled protein reveals that the rotational diffusion of squid rhodopsin in outer segment membranes that have been extensively washed with urea to remove peripheral proteins is much slower than that of bovine rhodopsin in rod outer segment disk membranes. This reduction in rotational mobility is also found with purified squid rhodopsin reconstituted in egg phosphatidylcholine and in urea-washed outer segment membranes which have been treated with endoprotease-V8 to remove the C-terminal extension of squid rhodopsin. In the latter case, the saturation-transfer ESR spectra are virtually identical to those of the non-proteolyzed membranes.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Células Fotorreceptoras/química , Rodopsina/química , Segmento Externo de la Célula en Bastón/química , Amidas , Animales , Bovinos , Membrana Celular/química , Citoplasma/ultraestructura , Decapodiformes , Espectroscopía de Resonancia por Spin del Electrón , Técnicas In Vitro , Microscopía Electrónica , Movimiento (Física) , Fosfatidilcolinas , Segmento Externo de la Célula en Bastón/ultraestructura , Espectrofotometría Infrarroja , Marcadores de Spin , Relación Estructura-ActividadRESUMEN
The sequence of squid (Loligo forbesi) rhodopsin was determined by protein and cDNA sequencing. The protein has close similarity to octopus rhodopsin, having an N-terminal region (residues 1-340) which resembles other guanine-nucleotide-binding protein (G-protein)-linked receptors and a repetitive proline-rich C-terminus (residues 340-452). Comparison of the sequence of squid rhodopsin with those of other members of the G-protein-linked receptor superfamily reveals features which we predict to have both structural and functional importance.
Asunto(s)
Rodopsina/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular/métodos , Decapodiformes , Proteínas de Unión al GTP/metabolismo , Datos de Secuencia Molecular , Sondas de Oligonucleótidos , Plásmidos , Conformación Proteica , Mapeo Restrictivo , Rodopsina/aislamiento & purificación , Rodopsina/metabolismo , Fosfolipasas de Tipo C/metabolismoRESUMEN
Bitter taste perception provides animals with critical protection against ingestion of poisonous compounds. In the accompanying paper, we report the characterization of a large family of putative mammalian taste receptors (T2Rs). Here we use a heterologous expression system to show that specific T2Rs function as bitter taste receptors. A mouse T2R (mT2R-5) responds to the bitter tastant cycloheximide, and a human and a mouse receptor (hT2R-4 and mT2R-8) responded to denatonium and 6-n-propyl-2-thiouracil. Mice strains deficient in their ability to detect cycloheximide have amino acid substitutions in the mT2R-5 gene; these changes render the receptor significantly less responsive to cycloheximide. We also expressed mT2R-5 in insect cells and demonstrate specific tastant-dependent activation of gustducin, a G protein implicated in bitter signaling. Since a single taste receptor cell expresses a large repertoire of T2Rs, these findings provide a plausible explanation for the uniform bitter taste that is evoked by many structurally unrelated toxic compounds.